Rapid Identification of Drug-Resistant Bacteria in Just 24 Hours! Our Chromogenic media offers exceptional specificity and sensitivity, ensuring swift and accurate identification of drug-resistant bacteria. - The media's colour-based differentiation provides a vivid colour distinction of target colonies, facilitating swift and efficient screening of culture plates. - Our media's high sensitivity and specificity instil technicians with greater confidence in the accuracy of reported positive cultures. - The streamlined confirmation process not only saves time and effort but also translates into reduced consumable usage, leading to financial savings. - The dehydrated format minimises wastage by enabling technicians to prepare plates as needed, enhancing efficiency and resource utilisation. Click the link if you want to learn more about how our media can improve efficiency in your laboratory https://bit.ly/4b8M6o0
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Making Life Science Research Easy! Enabling End-to-End Solutions to bring your solution to market and your vision to life
Blotchy blot? Undissolved milk solids could be the cause. Filter the blocking buffer for better results. Discover the secrets to obtaining great western blots. Utilize the provided images to pinpoint specific issues on your blots and quickly find effective solutions. Keep this guide within reach at your lab bench to steer clear of common mistakes and generate high-quality data you can publish. https://lnkd.in/d4vWBv-z #westernblot #westernblotting #westernblottingtroubleshooting #perfectblot #thermoscientific #invitrogen
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Assistant Professor | Outbreaks, Genomic Epidemiology, & Public Health
A new study in ICHE from Curtis Donskey and team shows just how difficult it is to keep sink drain bacteria under control. Bacteria from drains have been implicated in hospital outbreaks so reducing bioburden may help curb transmission. 🚿 The authors tried different frequency of drain cleaning and looked at CFU recovery of bacteria using 3.13% hydrogen peroxide and 0.05% peracetic acid disinfectant. "Our findings suggest that daily application may be required to achieve a rapid and consistent reduction in colonization. Application of the foam disinfectant every 2 days resulted in a downward trend in drain contamination, but suppression to below 2 log10 CFU only occurred after the sixth application" ❗ Daily application! Paper: https://lnkd.in/eckFEdSy
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The basic principle of Gram staining involves the ability of the bacteria cell wall to retain the crystal violet dye. Gram positive microorganisms have higher peptidoglycan content in the cell wall whereas Gram negative organisms have higher lipid content. Initially, all organisms take up the crystal violet dye but the use of ethanol (decolourizer) makes the lipid layer of Gram negative organisms to be dissolved and hence loose the primary stain. Factors that can lead to error in Gram staining reaction include * Excessive heat fixation: can damage the cell wall and make G-positive appear negative. * Over- decolourization: too long exposure to ethanol can remove dye from both G-positive and negative. * Thick smear preparation: can make G-negative not to be fully decolourized and therefore appear as positive. *Old/ weak reagents *Exposure of organisms to antibiotics. #microbiologicaltechniques #Gramstaining
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Microbiologist || Sterility Testing || ISO 9001:2015 (QMS)|| EMS|| cGMP || EHS || OHS|| Water Quality || Driving Compliance and Operation Excellence.
Water analysis: Pour plate vs Filtration method.
That's why it's better to use Filtration method than Pour-plate method in water analysis ;) #Elmicrobiologist Dr. Ahmed Mustafa
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Why Farmer Type Ionization Chamber Well Recommended For Absorbed Dose To Water In TRS-398 Though There Are Small Volume Chamber Available? 1. Using a Farmer-Type Chamber for Absorbed Dose to Water Measurements, Correction Factors needed to Determine as Outlined in TRS-398, is a Standard Practice due to its Well-characterized Response in Water. 2. While Smaller Volume Chambers like Pinpoint or Semiflex May Offer Higher Accuracy, but the Farmer-type Chamber's Established Dosimetric Properties Make It a Reliable Choice for Reference Dosimetry. 3. Additionally, Its Larger Volume Can Provide Better Water-Equivalence, Especially For Clinical Applications Where Depth Dose Information Is Crucial. If You Have Any Point to Add, Please Comment !! Thanks in Advance !
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Detecting bacteria: Group creates a unique method for quickly identifying different species of bacteria https://lnkd.in/gry9tYRX
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Bacteria are classified by their Gram stain characteristics. • Gram staining is the application of a crystal violet dye to a culture of bacteria. Bacteria that retain the color of the dye are called Gram positive; bacteria that don't are Gram negative. – The Gram stain attaches to peptidoglycan in the bacterial cell wall. • In Gram-negative bacteria, the peptidoglycan layer is protected by an outer membrane.
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🔶 Stock Solution preparation for preservation of microbiological samples : Using BHI media and glycerin, bacteriological and virological samples are preserved in stock solution. In BHI media, wide variety of fastidious microorganisms can be grown which are hard to grow elsewhere. Cryopreservatives like glycerol are used to store samples. 🟢 Requirments : 🔹BHI broth 🔹50% glycerin 🔹Cryovial 🔹Measuring cylinder 🔹Conical flask 🔹Beaker 🔹Distilled water 🔹Balance 🔹Autoclave machine 🔹Biosafety cabinet 🟢 Procedure : ▪️A solution of 50% glycerol and BHI solution at the optimal concentration is recommended. ▪️The solution and cryovial must be autoclaved ▪️Each cryovial is filled with 0.5ml BHI and 0.5ml glycerin. ▪️In order to use the stock solution later, it should be preserved at 4 degree celcius.
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Observing promising results usually alleviates fatigue and inspires us to enthusiastically take the next step :)! The picture depicts the qualitative assay for β-glucosidase production. In a medium containing esculin, bacteria that produce β-glucosidase can hydrolyze esculin to esculetin, which then reacts with ferric ions in the medium to produce a dark brown or black color.
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