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When a longer probe design is necessary, such as achieving a desired melting temperature (Tm) in an AT-rich target region, the increased distance between the fluorophore and quencher in standard end-labeled probes may result in reduced quenching efficiency. In double-quenched BHQnova Probes the nova quencher is closer to the fluorophore which serves to enhance quenching efficacy. Learn more in the video below: https://hubs.li/Q02q7Tnb0

Determining the affinity of strong ligand binders (Kd < µM) by NMR is often considered impossible. This is wrong, and here's why: 1) This mostly depends on the relationship between the exchange rate (kex) and chemical exchange (∆w); see the picture below. 2) Reaching low concentration is possible with hyperpolarization, including my favorite - photo-CIDNP. 3) Using reporters, especially with 19F labels, is an attractive solution to avoid going too low in concentration. I shared an example of that recently (in-cell NMR Kd determination. NMR is finally closing the gap between other methods, combining the different advanced tools, which will push the operative range of the technique to cover a broader spectrum of applications. #innovation #drugdiscovery

I am glad to share our latest published article! "Precision picric acid detection via a fluorenone-amide functionalized fluorescent micellar probe" in the Journal of Environmental Surfaces and Interfaces. https://lnkd.in/dNj2XfZi.

Discover the Future of Materials Analysis with TESCAN AMBER X2, advanced Plasma FIB-SEM system ... https://lnkd.in/ekKPf-un

The SUPR-DSF delivers next level differential scanning fluorimetry by simplifying sample preparation and analysis of protein stability into a single microplate. Utilising precision optical components to deliver the highest sensitivity, intrinsic fluorescence of proteins and full spectrum detection, both thermal ramping and isothermal chemical denaturation experiments can be easily run in 384-well microplates. Find out more: https://lnkd.in/eiS8XrR7 #ProteinStability #FluorescenceDetection #ThermalRamping

Did you know that there are two types of EDTA?  K2 and K3  What is the difference between them?  Do they affect the results? 🤔💭 Answer : Both contain the same substance, which is potassium, except that K3 has an additional potassium ion that enhances its ability to maintain cells. It is also a liquid located at the bottom of the can, while the other sees it as spray dots on the surface of the tube from the inside. K3 helps maintain the size of the cells. The rest of the differences are present. in the picture. hashtag#Medical_laboratories

How do cyclodextrins form a Complex? Since all the applications, all the advantages and drawbacks of using CDs go back to their ability to form non-covalent, dynamic complexes with guest compounds, it is essential to understand what drives the complex association and what triggers the dissociation, as well as to see what is the timescale this process takes. This is even more remarkable, once we learn that all industrially used CDs (irrespective of the industry itself) work this way - apart from a few exceptions. The primary mechanism by which cyclodextrins form inclusion complexes is through a process known as molecular encapsulation. In this process, the hydrophobic interior of the cyclodextrin molecule interacts with the hydrophobic portions of the guest molecule, while the hydrophilic exterior interacts with the hydrophilic portions of the guest molecule. This results in the formation of a stable complex that protects the guest molecule from degradation and improves its solubility. Want to learn more? https://lnkd.in/gW93CQ5H

Delighted to share our work on the Regioselective Metalation entitled as "Towards New Coordination Modes of 1,2,3-Triazolylidene: Controlled by the Nature of 1st Metalation in a Heteroditopic Bis-NHC Ligand" in online linkedin #RSCPoster 2024 conference. #RSCInorg #RSCPosterPitch Kindly Check the publication https://lnkd.in/gQ-nyZXC An unusual effect of the nature of first metal coordination of a heteroditopic N-heterocyclic carbene ligand towards the coordination behavior of 1,2,3-tzNHC is explored. The first metal coordination at the ImNHC site (complexes 3 and 4) was noted to substantially influence the electronics of the 1,2,3-triazolium moiety leading to an unprecedented chemistry of this MIC donor. Along this line, the RhIII/IrIII-orthometalation in complexes makes the triazolium C4-H more downfield shifted than C5-H, whereas a reverse trend, although to a lesser extent, is observed in case of the non-chelated PdII-coordination. This difference in behavior assisted us to achieve the selective activation of triazole C4/C5 positions, not observed before, as supported by the isolation of the homo- and hetero-bimetallic complexes, via C5- and C4-metalation, respectively. Further, the %Vbur calculation eliminates any considerable steric influence and the DFT studies strongly support the selectivity observed during bimetalation. Also, kindly check the related video https://lnkd.in/gnRM9hqT   

Did you know that there are two types of EDTA? K2 and K3 What is the difference between them? Do they affect the results? 🤔💭 Answer : Both contain the same substance, which is potassium, except that K3 has an additional potassium ion that enhances its ability to maintain cells. It is also a liquid located at the bottom of the can, while the other sees it as spray dots on the surface of the tube from the inside. K3 helps maintain the size of the cells. The rest of the differences are present. in the picture. #Medical_laboratories

Does Size Matter? (1 of 3) The size of the electrospray meniscus has to be small, but why? Mainly for three reasons. Here is one of them: To prevent electric discharges ⚡: When using smaller emitter tips, the electrospray reaches the set voltage before discharges occur. On the contrary, a larger emitter would cause discharges even before the spray is formed. Check out the video below to learn about meniscus size in electrospray! https://lnkd.in/dqG_rgx6