Drug Discovery Unit, University of Dundee’s Post

In the DDU’s latest paper Fraser Hughes and Alex Cookson from the our compound management team investigated an issue which impacts on the results of high through-put screening with compound libraries. Dry DMSO can rapidly pull water vapor out of the air due to its hygroscopic nature. This is a well-documented problem within drug discovery, particularly within high-throughput screening (HTS). This hydration is caused by atmospheric moisture being absorbed each time a compound library is used. This effect becomes increasingly pronounced when a compound library is used routinely. The result of this hydration is a change to both the total volume of solution and the concentration of sample still in solution. This can result in a large amount of variability in the measured biological activity of a sample depending on the library usage. In this paper, we show the detrimental effects the hydration of sample libraries has on the reproducibility of biological data and present a novel way to remove it from HTS library plates. Read their solution in SLAS Technology vol 29 https://lnkd.in/eVJgxz6S   We thank Roylan Developments Ltd and in particular Stuart Naylor for help with this project. #drugdiscovery #highthroughputscreening #compoundlibraries #methoddevelopment #publication

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