Enzo Life Sciences, Inc.’s Post

Host cell proteins (HCPs) are impurities produced during biotherapeutics manufacturing. While enzyme-linked immunosorbent assay (ELISA) is commonly used for overall HCP quantification, liquid chromatography–mass spectrometry (LC-MS) methods offer a complementary approach to profile individual HCPs, although they come with challenges. In this poster, we present case studies that highlights how we overcome challenges associated with LC-MS during downstream development. https://lnkd.in/gPe9wdEi #SamsungBiologics #cdmo #HCP #downstream #LC-MS 

Understanding the Critical Utility of Diluents in your Immunoassay:   Due to the variability within patient samples, different blocking strategies are needed to dilute samples to achieve maximum assay performance. For immunodiagnostic assays, a false positive or false negative result can lead to a misdiagnosis of a disease or condition and improper treatment decisions. Clinicians and patients depend on the in vitro diagnostics (IVD) industry to develop high quality assays that deliver accurate results consistently.   Surmodics IVD’s MatrixGuard™ Diluent and Assay Diluent (Protein-Free) are the gold standard for reducing false positives in your assay. These formulations provide two options to use when different methods are needed to block matrix interferences, while maintaining the clinical utility of the assay.   Visit the link below to learn more about the importance diluents have in building accurate and reliable immunoassays below and contact us below to request free samples of our diluents today.   https://lnkd.in/dkwhXS5h   https://lnkd.in/gwH7Ptk   SURMODICS, MATRXGUARD and the SURMODICS logo are trademarks of Surmodics, Inc. and/or its affiliates.   #diluents #immunoassay #elisa #diganostics #ivd #biotechnology

🎯 𝗗𝗼𝗲𝘀 𝗰𝗼𝗻𝗷𝘂𝗴𝗮𝘁𝗶𝗼𝗻 𝘀𝗶𝘁𝗲 𝗺𝗮𝘁𝘁𝗲𝗿 𝗳𝗼𝗿 𝗽𝗿𝗼𝘁𝗲𝗶𝗻 𝗰𝗼𝗻𝗷𝘂𝗴𝗮𝘁𝗲𝘀? 𝗣𝗮𝗿𝘁 𝗜𝗜 🎯 Site of conjugation influences the stability and pharmacokinetics of antibody-drug conjugates (ADCs). In "𝘓𝘰𝘤𝘢𝘵𝘪𝘰𝘯 𝘮𝘢𝘵𝘵𝘦𝘳𝘴: 𝘚𝘪𝘵𝘦 𝘰𝘧 𝘤𝘰𝘯𝘫𝘶𝘨𝘢𝘵𝘪𝘰𝘯 𝘮𝘰𝘥𝘶𝘭𝘢𝘵𝘦𝘴 𝘴𝘵𝘢𝘣𝘪𝘭𝘪𝘵𝘺 𝘢𝘯𝘥 𝘱𝘩𝘢𝘳𝘮𝘢𝘤𝘰𝘬𝘪𝘯𝘦𝘵𝘪𝘤𝘴 𝘰𝘧 𝘢𝘯𝘵𝘪𝘣𝘰𝘥𝘺-𝘥𝘳𝘶𝘨 𝘤𝘰𝘯𝘫𝘶𝘨𝘢𝘵𝘦𝘴" (𝘊𝘩𝘦𝘮. 𝘉𝘪𝘰𝘭. 20, 161–167), the researchers show that the site of conjugation significantly affects pharmacokinetics of ADCs. These studies reinforce a growing consensus: Conjugation site is a key determinant of ADC performance - impacting therapeutic activity, stability, and pharmacokinetics. At VALANX Biotech our technology allows you to freely choose and therefore optimize any conjugation site in a protein. We find the golden combination of antibody, payload and site for your program, strongly increasing the chance of building a successful ADC. 🥇 #Biotech #ProteinConjugation #ADCs #Innovation #LifeSciences #AntibodyDrugConjugates #XDCs #ConjugationScience

Transforming human iPSCs into hepatic cells requires tailored conditions at each differentiation phase, utilizing media enriched with particular growth factors and cytokines. Leveraging Sartorius Research Grade Growth Factors and Cytokines in combination with Incucyte®️ Live-cell Analysis and iQue®️3 High-Throughput Screening by Cytometry, we've established a straightforward protocol for creating mature hepatic organoids, streamlining the production of functional liver cells from iPSCs for research in drug development and toxicity testing. Learn more in our application note: https://ow.ly/3sxc50RzCfZ #ipscs #organoids #drugdiscovery #cellanalysis #livecellanalysis

05-2024. And here is #2: "Evaluating the Potential of Cyclodextrins in Reducing Aggregation of Antibody–Drug Conjugates with Different Payloads". Again, free download, JPharmSci! Just follow the link: https://lnkd.in/dwFzhgbi

Differentiating human iPSCs into liver cells involves specific stages and media, each with unique growth factors and cytokines. Using iQue®️3 High-Throughput Screening by Cytometry in combination with live-cell analysis and Sartorius Research Use Growth Factors, we've established a straightforward, standardized method for creating mature liver organoids, streamlining the production of functional liver cells from iPSCs for research in drug development and toxicity testing. Learn more in our application note: https://ow.ly/tX4B50Rhsjx #ipscs #organoids #drugdiscovery #cellanalysis #flowcytometry #hts

Small-Angle X-Ray Scattering as a Powerful Tool for Phase and Crystallinity Assessment of Monoclonal Antibody Crystallites in Support of Batch Crystallization Crystalline suspensions of monoclonal antibodies (mAbs) have great potential to improve drug substance isolation and purification on a large scale and to be used for drug delivery via high-concentration formulations. Crystalline mAb suspensions are expected to have enhanced chemical and physical properties relative to mAb solutions delivered intravenously, making them attractive candidates for subcutaneous delivery. This contribution describes a novel crystal structure of the antibody pembrolizumab and demonstrates the usefulness of small-angle X-ray scattering (SAXS) for characterizing its crystalline suspensions. https://lnkd.in/gEKUJ3qw #aspenalert #biotech #bioprocess

Elevate Your #Monoclonal Antibody #DrugDevelopment With Our #Pharmacokinetics And #Immunogenicity ELISA Services   🎯 Enhanced Sensitivity: Superior ELISA/ #MSD Platforms For Unmatched #MAb Signal And #Assay Performance 🧫 #Specificity & #Selectivity: Careful Characterization Of #CriticalReagents, Including #Antibody, #Antigen, Buffer, Blocker, Etc To Tackle #MatrixEffects  🧪 #SampleIntegrity: Comprehensive Guidance On #SampleCollection, Processing, And Storage To Maintain Sample Stability 📊 Optimized ELISA Protocol: Meticulously Fine-Tuned ELISA Workflow For Consistent And Reproducible Results 🌐 Engage With Us To Navigate Latest Regulatory Frameworks For ELISA #Bioanalysis. Consult Our Expert Scientists Today To Overcome Your #Immunoassay And #Bioassay Challenges. 🔗https://lnkd.in/dw2ra3KM

Now available to view on demand! In our recent webinar, experts present research strategies for the discovery of targeted protein degraders. Over the past decade, targeted protein degradation (TPD) technology has become one of the most promising methods to remove specific disease-related proteins using cellular self-destruction mechanisms. WuXi AppTec has built a comprehensive TPD discovery platform, adept in over 20 assay formats to investigate the full TPD functional cycle from binary/ternary complex formation and protein ubiquitination to degradation. Our broad and deep expertise also includes advanced screening techniques for hit identification, and custom synthesis and purification of new linkers and E3 ligase ligands. Click this link 👇 to view our webinar: https://lnkd.in/eYKU3sPz You can also view our related webinar, “Overcoming Key Challenges in PROTAC® Drug Development” by clicking here: https://lnkd.in/eA29G_nx #InducedProximity #TPD #PROTAC #ProteinDegraders #MolecularGlues

🔬 𝗕𝗿𝗲𝗮𝗸𝗶𝗻𝗴 𝗕𝗮𝗿𝗿𝗶𝗲𝗿𝘀 𝗶𝗻 𝗖𝘆𝗰𝗹𝗶𝗰 𝗣𝗲𝗽𝘁𝗶𝗱𝗲 𝗔𝗻𝗮𝗹𝘆𝘀𝗶𝘀: 𝗔𝘂𝗿𝗶𝗴𝗲𝗻𝗲 𝗗𝗠𝗣𝗞 𝗖𝗮𝘀𝗲 𝗦𝘁𝘂𝗱𝘆 Cyclic peptides present unique challenges in drug development due to their structural complexities. At Aurigene DMPK, we tackled these hurdles head-on in a recent project, ensuring precise analysis and reliable data.   𝗧𝗵𝗲 𝗣𝗿𝗼𝗯𝗹𝗲𝗺 𝗦𝘁𝗮𝘁𝗲𝗺𝗲𝗻𝘁 Analyze cyclic peptides for Kinetic Solubility, Chrom Log D, MDCK, PAMPA, and Caco-2 experiments, while addressing the intricate challenges of peptide cyclization in in vitro experiments and bioanalysis.   𝗧𝗵𝗲 𝗖𝗵𝗮𝗹𝗹𝗲𝗻𝗴𝗲𝘀 𝗪𝗲 𝗢𝘃𝗲𝗿𝗰𝗮𝗺𝗲 1️⃣ Nonspecific Binding (NSB) 2️⃣ Precipitation of peptides during DMSO stock spiking 3️⃣ pH-dependent compound stability 4️⃣ Low peptide recovery rates 5️⃣ Proteolysis in buffers and cellular enzymes 6️⃣ Inconsistent data   𝗢𝘂𝗿 𝗔𝗽𝗽𝗿𝗼𝗮𝗰𝗵 With a tailored strategy and advanced methodologies, we overcame these obstacles, delivering accurate, reproducible results to drive drug discovery forward.   🚀 At Aurigene DMPK, we thrive on solving complex problems. Want to learn how we can help accelerate your drug discovery programs? Let’s connect!  🔗 Learn more: https://lnkd.in/dFztHeZs 🔗 Contact us: contactapsl@aurigeneservices.com   #DrugDiscovery #PeptideAnalysis #CyclicPeptides #PharmaceuticalResearch #AurigeneDM