Gary Van Berkel’s Post

I am very happy to have been part of this great collaboration and even more so to finally see it out now! Our combined theoretical and experimental approach shows how FREE1, an ESCRT-accessory protein can mediate MVB formation. FREE1 condensate-membrane wetting can mediate ESCRT-independent membrane fission simply due to capillary forces. However, we also find that under stress conditions, the interplay between FREE1 condensates and the classic ESCRT pathways is indispensible for survival. Check the full story here: https://lnkd.in/eBZ6veQg

WOW, 1-min and 2-min effective graident for proteomics, this is not fast, this is ultra fast proteomics 😅 . A compensively-optimzed workflow based on several key steps, includes sample-prep automation, adaptive focused acoustics (AFA)-assisted proteolysis (very interesting), and narrow window DIA in Astral (not 2Da window, but 1 Da window 🤣 ), so in final, this is ~ 240 SPD (Most importent, the proteome coverage and depth are still very high). A wonderful work from scientists in MD Anderson and Thermo.

Review of #mRNA cap structures, cap analogs and NUDIX enzymes with known decapping activity (Nudt16, Nudt12, and Nudt2) that can be used as tools to characterize synthetic capped mRNAs.

Interesting read on the use of trapped-ion mobility spectrometry (TIMS) and parallel accumulation serial fragmentation (PASEF) available on Bruker TIMS tof Instruments for enhanced glycopeptide identification rates under complex mixtures. Thanks to careful optimisations of the critical instrument settings and the introduction of dopant-enriched nitrogen gas (DEN), up to 1500 glycopeptides were successfully identified based on 30-min LC separation.

Delve into the fascinating world of DNA amplification with our latest article comparing Loop-Mediated Isothermal Amplification (LAMP) and Polymerase Chain Reaction (PCR). Discover the nuances in mechanisms, temperature requirements, and sensitivities between these two powerful techniques. Whether you're a seasoned researcher or a curious enthusiast, this insightful read sheds light on the fundamental disparities shaping molecular biology today. Explore the possibilities and stay ahead of the curve with SBS Genetech. #LAMP #PCR #DNAamplification #MolecularBiology #SBSGenetech

Successfully completed a two day workshop on approaches on biochemical investigations held on 22nd & 23rd February 2024 . This workshop provided me with a comprehensive understanding of various biochemical investigational techniques, including: Qualitative analysis of biomolecules (monosaccharides, disaccharides, polysaccharides, proteins) Hematological studies (sample collection and preservation, separation of serum and plasma from blood, differential staining of RBCs and WBCs) Cytological studies (observation of different stages of mitosis and meiosis, calculation of mitotic and meiotic index)

Interesting paper by the groups of Haojie Lu and Ying Zhang in Nature Communications. A chemical probe to analyse lysines on the cell surface using an ortho-phthalaldehyde warhead and a disulfide linker that gets cleaved, if the probe enters the cytosol. https://lnkd.in/e-byi6Ua

In this poster presentation, we discuss how we developed and validated a sensitive LC-MS/MS assay for atropine, quantifying it across a wide range (80 pg/mL to 40 ng/mL) in rabbit plasma. Our method addresses the challenges of enantiospecific and species-specific atropinesterase, ensuring accurate achiral quantitation. Following ICH M10 guidelines, we achieved a 100% success rate in incurred sample reanalysis, confirming the robustness of our sample stabilization strategy. Watch it here: https://lnkd.in/dtUDwknU #MassSpectrometry #Bioanalysis #DrugDevelopment

https://lnkd.in/gNQyh5JD I'm delighted to share our recent publication in the Journal of the American Chemical Society (JACS), where we unveil the completion of apramycin biosynthesis. Under the leadership of Dr. Shusuke Sato, our team introduced an unconventional " β-D "-sugar nucleotide during the final glycosylation step. This breakthrough not only bridges a critical gap in the apramycin biosynthetic pathway but also highlights the first enzymatic glycosylation process accepting the β-D-sugar nucleotide as a substrate in the AprO-catalyzed reaction. For detailed characterization of other enzymes, including AprJ, AprK, AprD5, and AprL, please refer to our paper.

Spectroscopic Insights into NEET Proteins: Our study zooms in on CISD3's spectral behavior under stress conditions like iron deficiency and oxidative stress. From chemical shift perturbations to paramagnetic NMR, we unravel its dynamic responses, offering intriguing clues about its functions. Check out our recently published article to explore the captivating world of NEET proteins!