Glaucoma Physician’s Post

Thrilled to present my work at the #RSCPoster2024 LinkedIn Conference-2024 on the topic “Organocatalyzed diastereoselective cyclization of β-alkyl nitroolefins with alkylidene malononitriles: new approach to azetidine nitrones and isoxazoles.” Here, we have developed the methodology to synthesize highly strained azetidine nitrones with a tetra-substituted chiral carbon centre as well as fully substituted isoxazoles. We have treated β-alkyl nitroolefins and β-aryl/heteroaryl/alkyl-substituted alkylidene malononitriles in presence of a catalytic amount of DABCO at room temperature. After 1-2hour diastereoselective azetidine nitrons have formed with good to high yield and excellent group tolerance. We have successfully synthesized 33 substrates with a good diastereomeric ratio (up to ≤ 93:7). Further, by using inexpensive Brønsted acid catalyst in this reaction for 12 hours, a novel class of fully substituted isoxazoles have been formed with 24 examples. Reaction involved ring-expansion reaction via a cleavage of C-N bond and C-O bond formation. Check the article Link: https://lnkd.in/dxYDZHd2 #RSCOrg #RoyalSocietyofChemistry

Check out the latest blog from our occupiers, OGT! Discover expert tips on how to tackle high background fluorescence in your FISH assays. Learn insider techniques for optimising sample preparation, hybridisation conditions, and more. Read the full blog 👇 https://lnkd.in/eR5fcUFi #occupiers #ogtblog #cytocell #fishprobes

"I have been using #Cryolocks for years and recently published them in a paper on my 'Fast & Furious' vitrification method. This study showed that immature oocytes vitrified and warmed using a short protocol survived and resumed meiosis without compromising survival rates. Additionally, it significantly reduced the time needed for vitrification and warming. Cryolocks are easy to handle and load, reliable, robust, and easy to label using a Brady labeler. They are affordable and suitable for my lab’s clinical routine of egg vitrification." -Juergen Liebermann Check out the paper 👉 https://lnkd.in/gdtDBKF6

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NHCs can it generated insitu and used insitu. with cyclised thiourea. cyclised thiourea can be Naklyalted with various group it's titration which one works best. we can talk to expert about biteangle or concept of biteangle once we get catalyst from titration that works best. coupling partners. insitu generation of Carbene for coupling partners. just a thought.

Blotchy blot? Undissolved milk solids could be the cause. Filter the blocking buffer for better results. Discover the secrets to obtaining great western blots. Utilize the provided images to pinpoint specific issues on your blots and quickly find effective solutions. Keep this guide within reach at your lab bench to steer clear of common mistakes and generate high-quality data you can publish. https://lnkd.in/d4vWBv-z #westernblot #westernblotting #westernblottingtroubleshooting #perfectblot #thermoscientific #invitrogen

Spilling the secrets: How to count cells with a hemocytometer Hemocytometers are used to determine the total cell count and viability of many different cell types in various applications. In ChemoMetec, we've looked at hemocytometers and answered common questions, such as: ❔ What are hemocytometers and how do they work? ❔ How do I count cells using a hemocytometer? ❔ What rules and strategies should I use for manual cell counting? ❔ How do I calculate dilution factor, cell count, and viability? ❔ What common cell counting errors should I be aware of? To learn more, visit the link below. https://lnkd.in/ee53NctS

Unlike that of the Dynamic Applications, Molecular Sieves for Static Applications is for a one time use only. With full control from synthesis to activation, our range of Molecular Sieves ensures consistent efficacy and precision when it comes to adsorbing and trapping moisture, even at trace levels.   #udecgroup #zeolitepowder #molecularsieves

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