UC San Diego HUMANOID: Center of Excellence in Human Organoid Research reposted this
The UC San Diego Agilent Center of Excellence in Cellular Intelligence 's weekly series--#DidYouKnow--features examples of how you can push the limits of #Measurements-#Across-#Scales. This is our 14th post of this series. #DidYouKnow that aging #myofibroblasts that refuse to die are the bad actors that propel tissue #fibrosis practically any organ in our body? To solve such a problem, we can cut #time, #cost, and #effort by getting the most out of any study if we can combine #precision grouping of patients and multiple real-time #readouts. For example, last week we posted real-time #impedance + #apoptosis (imaging Annexin V) assays that provide a #dynamic, label-free method to monitor #cell #states, #responses to #stimuli or #drugs, and #changing #fates amidst a population of cells. Today's post (👇) continues to analyze that same ongoing experiment, and thereby, 'milk the cow' (proverbial) as we added two other #readouts, cellular #senescence (shown today) and #cytokine studies (not shown on supernatants collected). WHAT WAS DONE: This is a a novel #target [discovered using #MachineLearning among other computational approaches at the Institute for Network Medicine (iNetMed)] that selectively decimates #myofibroblasts that propel fibrostenotic #CrohnsDisease is being tested. Repeated daily doses of the drug (arrows) gradually bring the initial high #impedance of the pathology driving #profibrogenic #myofibroblasts (pink/magenta) similar to that of th benign type (blue). UC San Diego HUMANOID: Center of Excellence in Human Organoid Research worked closely with UC San Diego Center for Precision Computational Systems Network (PreCSN) to conduct deep characterization of--what appears to be a surprisingly consistent (and meaningful)--two molecular subtypes! Publications will follow. But the #drugdiscovery #workflow seems to be all set for now! Post credit: Harrison Penrose, Courtney Tindle, Ella McLaren, Mason Hayashi and Kevin Perry If you want to know more about how xCELLigence RTCA eSIGHT can help with your research, contact us at: Kevin Perry [k3perry@health.ucsd.edu] Link to learn more about the eSIGHT: https://lnkd.in/gAzDis7 How to #access these and other #equipment: https://lnkd.in/g2iChuYf Need help scheduling? Kevin Perry [k3perry@health.ucsd.edu]. #training; #measurement; #biology; #quantitative; #imaging; #drugdiscovery; #IBD, #fibrosis, #Crohnsdisease, #Myofibroblasts; #therapeutics; #bioenergetics; #metabolism. Salk Institute for Biological Studies La Jolla Institute for Immunology Scripps Research UC San Diego
