Incucyte® - Live-Cell Analysis Systems’ Post

New study introduces a screening method for identifying drugs that modulate the reactivity of astrocytes, cells implicated in neurodegeneration due to disease, injury, and aging. Histone deacetylase 3 (HDAC3) inhibitors were found to effectively reduce harmful astrocyte responses. The inhibitor RGFP966 specifically prevented the formation of reactive astrocytes and promoted neuroprotection in mouse models, highlighting the therapeutic potential of targeting astrocyte reactivity in neurodegenerative conditions. #Neurodegeneration #AstrocyteModulation #HDAC3Inhibitors #Neuroprotection #BiomedicalResearch #Neuroscience #TherapeuticStrategies https://lnkd.in/ei23Txdx

🧠 Mayo Clinic scientists have identified unique molecular signatures in the blood-brain barrier linked to Alzheimer’s disease. This breakthrough, published in Nature Communications, reveals that altered VEGFA and SMAD3 interactions between brain cells could serve as novel biomarkers for early diagnosis and new treatment targets. Utilizing brain tissue samples and advanced RNA sequencing, the team has paved the way for innovative therapeutic strategies. This discovery underscores the importance of understanding brain vascular changes in combating Alzheimer's. Dive deeper into this groundbreaking research and its potential to transform Alzheimer's diagnosis and treatment! #AlzheimersResearch #MedicalInnovation #Neuroscience #MayoClinic Read more: https://lnkd.in/gR6HPgHV

 Gold-plated neurons! ⭐️ Okay, no, not exactly, but how about gold nanoparticles as an alternative to optogenetics? Researchers at the University of Chicago have devised a method to stimulate neurons using gold nanoparticles (AuNPs) conjugated with antibodies. This approach is a real alternative to optogenetics as it allows you to induce neuron activation without genetic modification.  The team used antibodies, like our Anti-Rat TRPV1 (extracellular) Antibody (ACC-029) and Anti-P2X3 Receptor (extracellular) Antibody (APR-026) and conjugated them to AuNPs to target specific membrane proteins and localize photostimulation. They manually removed BSA and sodium azide from their antibody solutions to help with the conjugation. What they could have done was get the carrier-free versions from us at the start and save all that time and effort of doing it themselves! This work has significant potential in both research and therapeutic applications, especially when it comes to investigating neurological disorders and potentially restoring vision in degenerative eye diseases. Get all the details published in Neuron 👇 https://lnkd.in/eCJCJ9xp Cell Press University of Chicago University of Illinois Chicago Stephen Kent Bobo Dang David Pepperberg #nanoparticles #neuroscience #optogenetics #antibodies

𝗛𝘂𝗺𝗮𝗻 𝗕𝗿𝗮𝗶𝗻 𝗖𝗵𝗶𝗺𝗲𝗿𝗼𝗶𝗱𝘀 𝗶𝗻 𝗗𝗶𝘀𝗲𝗮𝘀𝗲 𝗥𝗲𝘀𝗲𝗮𝗿𝗰𝗵 - 𝗔𝗻 𝘂𝗽𝗱𝗮𝘁𝗲 𝘁𝗼 𝘁𝗵𝗶𝘀 𝗍𝗁𝖾𝗆𝖾 𝗳𝗿𝗼𝗺 𝗮 𝗉𝗈𝗌𝗍 𝖿𝗋𝗈𝗆 𝗳𝗲𝘄 𝘄𝗲𝗲𝗸𝘀 𝗮𝗴𝗼! A important survey finds that regulatory concerns are a major barrier to the adoption of non-animal testing models in research and development. Despite advances in alternative newer and increasingly more relevant in vitro methods, these models face significant challenges in gaining regulatory acceptance. #NonAnimalTesting #RegulatoryAffairs #R&D Read more here: https://lnkd.in/gxUncYXh 👌 A good work around that should be promoted: A new paper in Nature (https://lnkd.in/e7Dv7QiZ) shows that mixing cells from different individuals can help bypass the need for running different numbers at once.... Human Brain Chimeroids in Disease Research: 🧠 New Adapted Brain Model: Chimeroids, created by co-developing cells from multiple donors, replicate human brain development, allowing for diverse genetic representation. #Neuroscience #Innovation 🧪 Genetic Variation Study: This model investigates how genetic differences affect brain cell response to neurotoxins like ethanol or drugs such as valproic acid, showing variability in susceptibility among the same cells which came from different individuals. #Genetics #Research 🔬 High-Throughput Analysis: Chimeroids are a scalable system for examining individual differences in brain development and disease processes, enhancing our understanding of genetic impacts on health. #Bioinformatics #DiseaseResearch Such newer technologies will fuel research results with wider relevance and takes into account personalised medicine without having to resort to in vivo models.

Impact of CRISPR in drug discovery "CRISPR-based screens of cell lines, and phenotypic readouts such as cell growth, can facilitate the discovery of genetic interactions that could be therapeutic targets. Machine learning of these datasets and other information can be used to model drug effects on tumor microenvironments and better predict effects on patients." This technology can be applied to any disease, particularly for those with clear cellular phenotypes, such as lysosomal storage diseases. This Perspective article, focused on cancer drug discovery, was published in Science: https://lnkd.in/eR55ENje. #genetics #genomics #precisionmedicine #genomicmedicine #cancer #brain #neurology #neurodegeneration #neuroscience #neuroinflammation #lysosomalstoragedisease #drugdiscovery #drugdevelopment #ai #omics #biotechnology #pharma #innovation #research #science #sciencecommunication

🧠 4 posters to check out at SfN this week My colleagues Jeroen, Shushant, Peter, and Debbie are in Chicago right now for the annual meeting of the Society for Neuroscience! Find them at booth 368 to see how Ncardia can help you de-risk drug discovery with iPSC-derived models. You can also learn more in these posters: 🔶 Evaluation of human induced pluripotent stem cell (hiPSC)-derived tri-culture as in vitro model for Alzheimer’s Disease Board B60; In-Person Presentation Time: Sun., Oct. 6, 1-2pm. 🔶 Cellular and Molecular Mechanisms of Tauopathies, Synucleinopathies, and Other Degenerative Diseases Board C7; In-Person Presentation Time: Tue., Oct. 8, 10-11am 🔶 Leveraging an hiPSC-derived 3D tri-culture model to accelerate neuronal maturation and induce microglial dysfunction in a model of tauopathy Board B134; In-Person Presentation Time: Tue., Oct. 8, 10-11am 🔶 Development of iPSC-based Parkinson’s disease model for drug discovery Board B139; In-Person Presentation Time:  Tue., Oct. 8, 4-5pm #sfn2024 #neuroscience #drugdiscovery #ipsc

🧠🔬 New MLD Paper Alert! 🚀 📚 "Longitudinal Volumetric Analysis of Gray Matter Atrophy in Metachromatic Leukodystrophy" in Journal of Inherited Metabolic Disease! 🌟 Metachromatic Leukodystrophy (MLD) has long been known as a white matter disease, but our study reveals a previously overlooked aspect: the impact on gray matter. 🧠 Through comprehensive cross-sectional and longitudinal MRI analyses, we've uncovered significant atrophy in various brain regions of MLD patients, even post-treatment. 🔍 Key findings include: - Pronounced atrophy in cerebrum, cortical gray matter, and deep gray matter. - Notable correlations between motor and cognitive decline and brain atrophy. - Insight into the limited efficacy of hematopoietic stem cell transplantation (HSCT) on gray matter preservation. This research sheds new light on the complexity of MLD and underscores the importance of addressing gray matter pathology for comprehensive treatment strategies. 💡 Ready to delve deeper into the intersection of neuroscience and clinical care? Join the conversation and read the full paper now via https://lnkd.in/eNFtUdUu ! 📖 #MLDResearch #Neuroscience #GrayMatter #SciencePublication By: Murtadha Al-Saady, Hristina Galabova, Daphne Schoenmakers, Shanice Beerepoot, Caroline Lindemans, Peter Marin Van Hasselt, Marjo van der Knaap, Nicole Wolf, and Petra Pouwels

Don't forget to register for our webinar tomorrow to learn what multiplex cell phenotyping tells us about malignant brain tumors! 🧠 Attendees will also learn: - How to build a robust phenotyping panel informed by single-cell RNA-seq data - How to choose and establish essential biomarkers for the investigation of cellular hierarchies in complex brain tumor tissues - Factors to consider in evaluating multiplex platforms and technologies to optimize your research needs Register at your preferred time on April 25, 2024: ✔️ Europe: 7 AM EDT, 12 PM BST, 1 PM CEST, 7 PM CST https://bit.ly/4avdSuk ✔️ North America: 9 AM PDT, 12 PM EDT, 5 PM BST, 6 PM CEST https://bit.ly/3PY4MOc #glioma #gliomas #neuroscience #spatialbiology #multiplex

3D Model Brains from Multiple Donors: Key Insights 🧠 Innovative Brain Models Researchers have developed 3D brain organoids using cells from multiple donors, with the aim to reveal individual variations in brain development and drug responses. #BrainModels #Organoids 🔬 Chimeroid Technology These "Chimeroids" combine cells from up to five donors, potentially from hundreds in the future, enhancing genetic diversity in brain studies. #Chimeroids #GeneticDiversity 👩🔬 Drug Response Testing This model could predict individual responses to new therapeutics, providing a powerful tool for personalised medicine. #PersonalisedMedicine #DrugTesting 📚 Methodology The approach involves creating single-donor organoids, which are then homogenized and pooled to form composite organoids, enabling detailed neurophysiological and molecular studies. #ResearchMethods #Neuroscience Altogether, it looks very labor intensive and will be great to 'multiplex' starting donor cells so one can backtrack differences in response to treatment. It may also allow one to have different n number replicates in one go! Source: https://lnkd.in/emk7vpVn Image source: Antón-Bolaños et al./Nature

I'm excited to share our latest research: "A Dynamic Study of VEGF-A siDOX-EVs Trafficking Through the In-Vitro Insert Co-Culture Blood-Brain Barrier Model by Digital Holographic Microscopy" This study dives into the intricate processes of how VEGF-A siDOX-EVs navigate the blood-brain barrier. Utilizing digital holographic microscopy, we've uncovered new insights that could pave the way for breakthroughs in treating neurological conditions. our research's key Findings: • Enhanced understanding of VEGF-A siDOX-EVs behavior. • Potential implications for drug delivery systems targeting the brain. • Advanced imaging techniques to observe real-time cellular interactions. And thanks to my team and colleagues who made this research possible. Your dedication and expertise have been invaluable. I'd love to hear your thoughts on our findings and discuss potential impacts on the field. #Neurooncology #Neuroscience #BBB #Drugdelivery #DigitalHolography