International Society for Extracellular Vesicles’ Post

Excited to share our latest published paper in the Journal of Proteome Research! 📚 Our study delves into the metabolic intricacies of the small intestine, shedding light on regional differences and the implications for human health. Using untargeted proteomics, we unveil fascinating insights, including correlations between mouse ileum proteins and human gene expressions, as well as discoveries related to lysosomal acid lipase (LAL) deficiency and its effects on intestinal lipid metabolism. Our findings offer novel perspectives on gastrointestinal health and potential markers for LAL deficiency and systemic inflammation. Grateful for the collaborative effort that made this study possible! Dive into the details here: https://lnkd.in/diCYTmHS #Research #Proteomics #Metabolism #HealthScience"

Among others, the conclusions of this study are: "Our study raises the possibility that GPNMB and sTREM2 may be used as markers for LAL-D and/or systemic inflammation in LAL-D, which should be investigated in LAL-D patients in the future." Thank you Valentina Bianco for sharing this invaluable information about research on Lysosomal Acid Lipase Deficiency! It's essential to disseminate knowledge about this ultra-rare and complex disease to stimulate medical advancements that can enhance the lives of those affected by it. This information will undoubtedly contribute to a brighter future for individuals grappling with this condition. #AELALD #LALD #LAL_D #LALDeficiency #LysosomalAcidLipaseDeficiency #WolmanDisease #CESD #LivingWithLALD #LALDPower #Kanuma #SebelipaseAlpha #LysosomalDisease #LysosomalStorageDisorders #LSD #MetabolicDisorders #MetabolicDiseases #LysosomalLipidStorage #Hypercholesterolemia #Lipidosis #Hepatitis #LiverDisease #LDL #Cholesterol  #RareDiseases #NBS #NewBornScreening

In this recently published study, administration of either a live or heat-killed (10^9 CFU/day) strain of L. reuteri (ATCC PTA-6475) maintained bone volume and structure in an ovariectomized mouse model. No significant changes in intestinal Occludin or pro-inflammatory cytokine gene expression were observed potentially suggesting other mechanisms for the bone health benefits. It would be interesting to determine if there is a dose response for the benefits observed. It would also be interesting to evaluate if supplementation with other #probiotic strains in either their living or #postbiotic form would result in similar findings. The effects of supplementing #animalfeed with probiotics and postbiotics on bone health appears be an area worthwhile for further exploration, especially from a longevity perspective. #probiotics #postbiotics #animalnutrition https://lnkd.in/gKCCYRd4

Here is a recent review published in Microbiological Research on the role of extracellular vesicles in bacteria. More needs to be done on this topic, and we hope that this review may foster new ideas! Take a look! #bacteria #microbiology #extracellularvesicles #physiology #gutmicrobiota #health #research

📃Scientific paper: Composition of mucus- and digesta-associated bacteria in growing pigs with and without diarrhea differed according to the presence of colonic inflammation Abstract: Background In the pig production, diarrhea can occur during different growth stages including the period 4–16 weeks post weaning, during which a diarrheal outbreak also termed as colitis-complex diarrhea (CCD) can occur and it is distinct from post-weaning diarrhea (1–2 weeks post weaning). We hypothesized that CCD in growing pigs is associated with changes in colonic microbiota composition and fermentation patterns, and the aim of the present observational study was to identify changes in digesta-associated bacteria (DAB) and mucus-associated bacteria (MAB) in the colon of growing pigs with and without diarrhea. A total number of 30 pigs (8, 11, and 12 weeks of age) were selected; 20 showed clinical signs of diarrhea and 10 appeared healthy. Based on histopathological examination of colonic tissues, 21 pigs were selected for further studies and classified as follows: without diarrhea, no colon inflammation (NoDiar; n  = 5), with diarrhea, without colonic inflammation (DiarNoInfl; n  = 4), and with diarrhea, with colonic inflammation (DiarInfl; n  = 12). Composition (based on 16S rRNA gene amplicon sequencing) and fermentation pattern (short-chain fatty acids; SCFA profile) of the DAB and MAB communities were characterized. Results The DAB showed higher alpha diversity compared to MAB in all pigs, and both DAB and MAB showed lowest alpha diversity in the DiarNoInfl group. Beta diversity was significantly different between DAB and MAB as well as between diarrheal group... Continued on ES/IODE ➡️ https://etcse.fr/kO6x ------- If you find this interesting, feel free to follow, comment and share. We need your help to enhance our visibility, so that our platform continues to serve you.

📃Scientific paper: Composition of mucus- and digesta-associated bacteria in growing pigs with and without diarrhea differed according to the presence of colonic inflammation Abstract: Background In the pig production, diarrhea can occur during different growth stages including the period 4–16 weeks post weaning, during which a diarrheal outbreak also termed as colitis-complex diarrhea (CCD) can occur and it is distinct from post-weaning diarrhea (1–2 weeks post weaning). We hypothesized that CCD in growing pigs is associated with changes in colonic microbiota composition and fermentation patterns, and the aim of the present observational study was to identify changes in digesta-associated bacteria (DAB) and mucus-associated bacteria (MAB) in the colon of growing pigs with and without diarrhea. A total number of 30 pigs (8, 11, and 12 weeks of age) were selected; 20 showed clinical signs of diarrhea and 10 appeared healthy. Based on histopathological examination of colonic tissues, 21 pigs were selected for further studies and classified as follows: without diarrhea, no colon inflammation (NoDiar; n  = 5), with diarrhea, without colonic inflammation (DiarNoInfl; n  = 4), and with diarrhea, with colonic inflammation (DiarInfl; n  = 12). Composition (based on 16S rRNA gene amplicon sequencing) and fermentation pattern (short-chain fatty acids; SCFA profile) of the DAB and MAB communities were characterized. Results The DAB showed higher alpha diversity compared to MAB in all pigs, and both DAB and MAB showed lowest alpha diversity in the DiarNoInfl group. Beta diversity was significantly different between DAB and MAB as well as between diarrheal group... Continued on ES/IODE ➡️ https://etcse.fr/kO6x ------- If you find this interesting, feel free to follow, comment and share. We need your help to enhance our visibility, so that our platform continues to serve you.

📚 Exciting News! 🎉 Delighted to share that my thesis paper titled “The HtrA chaperone monitors sortase-assembled pilus biogenesis in Enterococcus faecalis” has been accepted and published in Plos Genetics. This research investigates the role of the high temperature requirement A (HtrA) chaperone/protease in E. faecalis, focusing on its function in clearing off-pathway pili and its implications for bacterial virulence. 🔍 In our study, we found that in E. faecalis, HtrA plays a crucial role as a quality control system, clearing aberrant off-pathway pili from the cell membrane in the absence of sortase A (SrtA). This process is vital as accumulation of these membrane-bound pili leads to cell envelope stress, triggering the regulon of the ceftriaxone resistance-associated CroRS two-component system. This system responds by inducing hyper-piliation and cell morphology alterations, impacting bacterial resistance against cephalosporins and daptomycin. 🔬 Our findings provide new insights into how E. faecalis responds to endogenous membrane perturbations, highlighting the intricate interplay between HtrA, pilus biogenesis, and the CroRS system. 📊 For more details, you can read the paper here: https://lnkd.in/gDSVrc9u Special thanks to Kimberly Kline, co-author Cristina Colomer Winter, PhD and Kline lab team at SCELSE for pushing it through the final lap! #ResearchPublication #Microbiology #BacterialVirulence

📃Scientific paper: Adjunctive efficacy of Bifidobacterium animalis subsp. lactis XLTG11 for functional constipation in children Abstract: Functional constipation (FC) can seriously affect the physical and mental health of children. The goal of this study is to assess the efficacy and safety of Bifidobacterium animalis subsp. lactis XLTG11 in treating FC in children through a randomized, double-blinded, placebo-controlled approach. Eligible children were randomized into either the intervention group (IG, n  = 65, receiving conventional treatment with probiotics) or the control group (CG, n  = 66, receiving conventional treatment without probiotics). The primary outcome measure was fecal frequency. Fecal gut microbiota analysis and PICRUSt (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) were used to predict gene family abundances based on 16S information. Over the course of treatment, the weekly frequency of feces within each group increased significantly ( F  = 41.97, p  < 0.001). The frequency of feces (times/week (t/w)) in the IG was significantly higher than that in the CG (3.69 ± 2.62 t/w vs.3.18 ± 1.43 t/w, 4.03 ± 2.54 t/w vs. 2.89 ± 1.39 t/w and 3.74 ± 2.36 t/w vs. 2.94 ± 1.18 t/w and 3.45 ± 1.98 vs. 3.17 ± 1.41 t/w for the 1st, 2nd, 3rd, and 4th week after intervention, respectively) ( F  = 7.60, p  = 0.0067). After the intervention, dominate species shifted to Bifidobacterium longum , Bifidobacterium breve , and Escherichia coli in the IG. Additionally, genes related to short-chain fatty acid (SCF) metabolism were upregulated, while methane metabolism was down... Continued on ES/IODE ➡️ https://etcse.fr/emK ------- If you find this interesting, feel free to follow, comment and share. We need your help to enhance our visibility, so that our platform continues to serve you.

Very interesting paper on the role of proteasome membrane-tethering in cellular protein homeostasis (link below). The authors elegantly demonstrate that a subset of 26S proteasomes associates with the cellular endomembrane system via myristoylation of the proteasome subunit Rsp2 (myristoylation is a covalent modification of protein with myristate, a fatty acid). Once localised to cellular membranes, these membrane-associated proteasomes (MAPs) facilitate the dynamic turnover of integral and peripheral membrane proteins, consequently affecting a variety of absolutely critical cellular processes. One of these functions affected by MAPs is membrane protein trafficking and sorting and mutating the myristoylation motif in Rsp2 results in suppressed levels of certain pathologically relevant proteins at the cell surface and their apparent diversion into lysosomal compartments. Not surprisingly then, myristoylation-deficient Rsp2 causes embryonic lethality in mice (100% penetrance), highlighting the important role of MAPs in early mammalian development.   This impressive piece of work certainly paves the way to answer several follow-up questions on the fundamental biology of MAPs. First of all, despite Rsp2 being one of the most abundant myristoylated proteins across multiple mammalian cell types, only a small fraction of proteasomes containing the lipidated subunit actually localise to membranes at any one time. What differentiates this sub-fraction? Furthermore, if myristoylation alone is insufficient for membrane-association, what other factors may contribute? How is the myristate group shielded from hydrophilic interactions of cytoplasmic proteasomes? How dynamic is the process of proteasomes associating with cell membranes – can more proteasomes be specifically recruited to membrane compartments via their myristoyl groups under an increased demand for membrane protein degradation? #proteins #proteome #membranes #proteomics #proteostasis #cellbiology

📃Scientific paper: Adjunctive efficacy of Bifidobacterium animalis subsp. lactis XLTG11 for functional constipation in children Abstract: Functional constipation (FC) can seriously affect the physical and mental health of children. The goal of this study is to assess the efficacy and safety of Bifidobacterium animalis subsp. lactis XLTG11 in treating FC in children through a randomized, double-blinded, placebo-controlled approach. Eligible children were randomized into either the intervention group (IG, n  = 65, receiving conventional treatment with probiotics) or the control group (CG, n  = 66, receiving conventional treatment without probiotics). The primary outcome measure was fecal frequency. Fecal gut microbiota analysis and PICRUSt (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) were used to predict gene family abundances based on 16S information. Over the course of treatment, the weekly frequency of feces within each group increased significantly ( F  = 41.97, p  < 0.001). The frequency of feces (times/week (t/w)) in the IG was significantly higher than that in the CG (3.69 ± 2.62 t/w vs.3.18 ± 1.43 t/w, 4.03 ± 2.54 t/w vs. 2.89 ± 1.39 t/w and 3.74 ± 2.36 t/w vs. 2.94 ± 1.18 t/w and 3.45 ± 1.98 vs. 3.17 ± 1.41 t/w for the 1st, 2nd, 3rd, and 4th week after intervention, respectively) ( F  = 7.60, p  = 0.0067). After the intervention, dominate species shifted to Bifidobacterium longum , Bifidobacterium breve , and Escherichia coli in the IG. Additionally, genes related to short-chain fatty acid (SCF) metabolism were upregulated, while methane metabolism was down... Continued on ES/IODE ➡️ https://etcse.fr/emK ------- If you find this interesting, feel free to follow, comment and share. We need your help to enhance our visibility, so that our platform continues to serve you.