IPRASENSE’s Post

A recent study has identified an 18-amino acid sequence that enables proteins to bind to exosomes, facilitating their transport throughout the body. This breakthrough, published in Science Advances, holds significant potential for exosome therapy, which aims to utilize exosomes for drug delivery. The research focused on the protein Wnt7a, crucial for development and regeneration, and identified the Exosome Binding Peptide (EBP) responsible for exosome targeting. This discovery could revolutionize drug development, particularly for conditions like Duchenne muscular dystrophy, by overcoming previous challenges in protein delivery across the body.

Recent findings from The Ottawa Hospital and the University of Ottawa have unveiled an 18-amino acid sequence, termed the Exosome Binding Peptide (EBP), which enables proteins to attach to exosomes for efficient transport throughout the body. This breakthrough, detailed in Science Advances, holds significant potential for exosome therapy, a promising avenue for drug delivery. By leveraging this discovery, researchers can enhance the delivery of therapeutic proteins, such as Wnt7a, which has been challenging to distribute due to its lipid composition. This advancement could expedite drug development for conditions like Duchenne muscular dystrophy.

✅ Fe3O4 nanoparticles were synthesized using the medicinal plant Malva sylvestris through a facile synthesis method. ✅ The synthesized nanoparticles were characterized through surface morphology, functional groups, and elemental analysis. ✅ The synthesized nanoparticles exhibited cytotoxicity against MCF-7 and Hep-G2 cancer cell lines, with IC50 values of 100 µg/mL and 200 µg/mL, respectively. ✅ The synthesized nanoparticles demonstrated antibacterial performance against four bacterial strains: Staphylococcus aureus, Corynebacterium, Pseudomonas aeruginosa, and Klebsiella pneumoniae. ✅ The nanoparticles showed higher antibacterial effects against Gram-positive strains (Staphylococcus aureus and Corynebacterium) with minimum inhibitory concentration (MIC) values of 62.5 µg/mL and 125 µg/mL, respectively, increasing the inhibitory percentage to more than 90%. Link: https://lnkd.in/dSyraMw Link: https://lnkd.in/gVHfzxDb #Fe3O4nanoparticles #Greensynthesis #Malvasylvestris #Characterization #Cytotoxicity #Anticancer #Antibacterial #Minimuminhibitoryconcentration(MIC) #Grampositivebacteria #Biomedicalapplications

🔬 Understanding cell potency: multipotent, totipotent, pluripotent cells. Dive into the distinctions: ➡ Multipotent cells specialize. ➡ Totipotent cells are ultra-versatile. ➡ Pluripotent cells offer broad potential. Join the discussion on cell potency levels or explore stem cell differentiation further! Let's broaden our understanding together. #CellBiology #StemCells #Research #researchanddevelopment #biotech #biotechnology #sciencecommunication #preclinical #preclinicalresearch

Delighted to see our collaborative paper exploring the regenerative properties of #mesenchymalstromalcell derived #extracellularvesicles (MSC-EV) published in MDPI Bioengineering. This project gave me the opportunity to explore the fascinating potential of MSC-EVs as regenerative therapeutics. 🔎 In this study, we assessed MSC-EV-treated human osteoarthritic chondrocytes (OACs) viability, proliferation, migration, cytokine and catabolic protein expression, and microRNA and mRNA profiles. 💡 Our data provide new insights on the potential mechanism of action of MSC-EVs, which may pave the way for more targeted novel therapeutics. It has been a truly collaborative effort alongside Xiao Nong Wang and Elena Jones, with secondments from Clara Sanjurjo-Rodríguez and Annachiara Scalzone, valuable input from Newcastle University Flow Cytometry Core Facility (FCCF), BioImaging Unit and Electron Microscopy Research Services, and funding from Versus Arthritis Tissue Engineering & Regenerative Therapies Centre. You can read the full article here: https://lnkd.in/edbiQsi5

The biosynthesis of materials using medicinal plants can be a low-cost and eco-friendly approach due to their extraordinary properties. Herein, we reported a facile synthesis of Fe3O4 nanoparticles using Malva sylvestris. The surface morphology, functional groups, and elemental analysis were done to characterize the synthesized nanoparticles. The cytotoxicity performance of the synthesized nanoparticles was analyzed by exposing nanoparticles to MCF-7 and Hep-G2 cancer cell lines through MTT colorimetric assay and the IC50 value was defined as 100 µg/mL and 200 µg/mL, respectively. The antibacterial performance of synthesized nanoparticles against four different bacterial strains including Staphylococcus aureus, Corynebacterium, Pseudomonas aeruginosa, and Klebsiella pneumoniae were assessed through microdilution broth method. The synthesized Fe3O4 nanoparticles using Malva sylvestris demonstrated higher antibacterial effects against Gram-positive strains with MIC values of 62.5 µg/mL and 125 µg/mL which increase the inhibitory percentage to more than 90%. Data on cytotoxic and antibacterial activity of synthesized Fe3O4 nanoparticles using Malva sylvestris DOI: 10.1016/j.dib.2019.104929 https://lnkd.in/dSyraMw

📢 Publication Alert! Fresh off the press – thrilled to share that one of my PhD projects has been published in #ACS Applied Bio Materials 🌟 In this study, we explored the potential of Zinc-based #MOFs as #nonviral vectors for #siRNA delivery to prostate cancer cells. We found that the imidazole content plays a crucial role in forming well-defined, highly #crystalline ZIF structures, with the #optimal_ratio significantly influencing crystalline structure, particle size, and shape of the siRNA-loaded biocomposites. We collaborated with Fiona Elsey Cancer Research Institute to find #inflammatory responses over extended periods to demonstrate Zn-MOF as safe and promising vectors for siRNA delivery. Read our work here: https://lnkd.in/gYAY-Bbb   With a strong foundation in biomaterials, advanced materials, and vector systems for drug/nucleic acid delivery, I’m eager to bring my expertise as I’m actively looking for #postdoc position to conduct impactful research in healthcare-related projects. If you know of any opportunities or have insights on collaborative projects, I’d love to connect and discuss. Ravi Shukla, PhD Arpita Poddar Aparna Jayachandran RMIT University RMIT School of Science #GeneTherapy #Biotechnology #MolecularBiology #PostdoctoralResearch #Postdoc #DrugDelivery #AntimicrobialResearch #CellBiology #Chemistry #Biotech      

BioRestorative Therapies, Inc. reported that it has had substantive discussions with an undisclosed commercial stage regenerative medicine company with regard to a license of BioRestorative’s allogeneic, off-the-shelf ThermoStem ® metabolic intellectual property. Previously published peer-reviewed preclinical data from a study conducted in collaboration with the University of Utah School of Medicine demonstrated a clonogenic population of metabolically active brown adipose tissue (“BAT”) stem cells residing in adult humans that: 🔵Can be expanded in vitro 🔵Exhibit multilineage differentiation potential (osteogenetic, chondrogenic and adipogenic) 🔵 Functionally differentiate into metabolically active brown Adipocytes. 👉Read the full press release here: https://lnkd.in/gWThCrwk

#paper "Cholesterol Conjugated Elastin-like Recombinamers: Molecular Dynamics Simulations, Conformational Changes, & Bioactivity." 🔬 Researchers have developed innovative cholesterol-functionalized biomaterials that enhance stability and enable the formation of biocompatible nanoaggregates. 💊 This breakthrough paves the way for new drug delivery strategies, particularly in cancer therapy and immune modulation. 🧪 Combining molecular dynamics simulations (GROMACS) & experimental assays, the study confirms efficient membrane interaction & internalization. 📄 This work was conducted in collaboration among iMATUS, the Bio2Eng Group, and the 3B’s Research Group. Authors: Rui Costa, Vicente Domínguez Arca, Brenda Velasco, Rui L. Reis, José Carlos Rodríguez Cabello, Iva Pashkuleva, Pablo Taboada Antelo and Gerardo Prieto. 🔗 https://lnkd.in/dGijugS5 #Biomaterial #DrugDelivery #CancerTherapy

Recent advancements in protein delivery have been made with the discovery of an 18-amino acid sequence that enables proteins to bind to exosomes, facilitating their transport throughout the body. This breakthrough, published in Science Advances, has significant implications for exosome therapy, a promising approach for drug delivery. By identifying the Exosome Binding Peptide (EBP) within the protein Wnt7a, researchers have unlocked new potential for developing treatments for diseases like Duchenne muscular dystrophy. This discovery addresses previous challenges in protein delivery, paving the way for innovative drug development strategies.