ISS inc.’s Post

Read the story on how we seamlessly integrated the PDA-23 detection module from Pi Imaging Technology with our Luminosa microscope, enabling the powerful combination of Image Scanning Microscopy (ISM) and Fluorescence Lifetime Imaging (FLIM). #microscopy #ism #fluorescence #flim 

The understanding of living systems at the nanoscale is significantly hindered by the limitations of current imaging techniques. A study conducted by Kerda Keevend, Toon Coenen, and Inge K. Herrmann discusses the advancements and potential of correlative cathodoluminescence electron microscopy (CCLEM) in bioimaging, particularly focusing on the development of nanometric resolution imaging for single protein labeling within a cellular ultrastructural context. Here are the top 5 key findings and takeaways from the review.  #Bioimaging #CCLEM #NanoscaleImaging #SingleProteinLabeling #BiomedicalResearch

Check out this amazing feedback from Prof. Friedemann Kiefer, Nikon Microscopy Ambassador and Director and Professor of Intravital Molecular Imaging at EIMI Muenster 🗣 "One of the beauties of this AX R MP instrument is its combination with a traditional confocal capability. This makes complicated experiments, like photo-conversion of fluorescent proteins in a small part of the tissue and large volume image acquisition, much simpler." Watch his full interview here ▶ https://lnkd.in/ecvG9JSM #nikonmicroscopy #confocal #microscope

🚀 New Success Story: Unleashing Unprecedented Resolution and Contrast in Confocal FLIM with the PDA-23 Array 🔬✨💻 In the constantly evolving world of microscopy, researchers are seeking new tools to unlock deeper insights into cellular dynamics and molecular interactions. Pi Imaging, a leader in advanced photon-counting solutions based on SPAD technology, has answered this call with the introduction of the PDA-23 array detector. ⭐ Download the Success Story Now: https://lnkd.in/egjBTHZP The PDA-23 is seamlessly integrated with PicoQuant’s Luminosa microscope, which enables combining the groundbreaking Image Scanning Microscopy (ISM) with Fluorescence Lifetime Imaging (FLIM). This module combines enhanced spatial resolution, improved contrast, and functional FLIM imaging, opening new frontiers. Discover the transformative power of our SPAD detector array and how the PDA-23 can elevate your microscopy research. Contact Pi Imaging: info@piimaging.com. 📩 Image Credits: PicoQuant #PiImaging #FLIM #microscopy #imaging #confocal #SPAD #PDA23 #PicoQuant #Luminosa

#Collaborative correlated imaging Confocal WITec Raman microscopes can be integrated with AFM, SEM, SNOM, or Raman-based particle analysis as correlative systems for investigating chemical and structural sample properties.

📝New scientific results from #QSNP partners! Robustness of chaotic-light correlation imaging against turbulence In this article, our partners giovanni scala, Cosmo Lupo and their colleagues from Politecnico di Bari and Università degli Studi di Bari consider an imaging scheme, inspired by microscopy, in which both correlation imaging and direct intensity imaging can be performed simultaneously, to investigate the effects of strong turbulence on the two different images. The theoretical comparison between direct and correlation imaging in the presence of strong turbulence unambiguously reveals an advantage of the latter. Remarkably, this advantage, quantified by analyzing the visibility of periodic sample patterns, is more striking when the presence of turbulence becomes the dominant factor in determining the image resolution. 🔗Read more about it here https://lnkd.in/gpRbdQrk

𝐈𝐧𝐭𝐫𝐨𝐝𝐮𝐜𝐢𝐧𝐠 | 𝐖𝐢𝐝𝐞𝐟𝐢𝐞𝐥𝐝 𝐎-𝐏𝐓𝐈𝐑™ We are happy to announce a new technique: Widefield O-PTIR™. This innovative sub-500 nm IR high-speed widefield chemical imaging mode offers a unique fluorescent-detection method, enabling you to image fluorescent samples in an entirely new way. Key Features of Widefield O-PTIR™: • Sub-500nm spatial resolution IR chemical imaging & spectroscopy • Single wavelength images captured in seconds; hyperspectral arrays in minutes • Dynamic chemical imaging with up to 5 frames per second • Fast measurement capabilities of fluorescently labeled and autofluorescent samples • Measurement of unlabeled and IR-tagged samples with standard O-PTIR microscopy • Co-located fluorescence microscopy combined with sub-micron IR spectroscopy Widefield O-PTIR™ opens new avenues for research and exploration across various applications, from cellular and bacterial studies to tissue analysis and beyond. The Widefield O-PTIR mode is available on the mIRage-LS product, for more information, visit our website https://lnkd.in/eFjPAp4t #WidefieldOPTIR #OPTIR #Fluorescence #IRspectroscopy #mIRageLSwidefieldOPTIR

White light diffraction phase microscopy for imaging of red blood cells for different storage times https://lnkd.in/dBcjmKux

ISS:40 - 1984 : 2024 Celebrating 40 Years of ISS In 2023, ISS continued its legacy of pushing the boundaries of fluorescence imaging by introducing the Parallel 16-PMT Spectral FastFLIM. This groundbreaking technology revolutionizes confocal scanning microscopy by adding the fluorescence spectrum and lifetime dimensions (λ, τ) to traditional imaging dimensions (x, y, z, t). The result? A six-dimensional dataset that unlocks unparalleled insights into complex biological systems. Here’s what sets our Parallel 16-PMT Spectral FastFLIM apart: Parallel Spectral FLIM Readouts: Achieve fast, unbiased spectral FLIM data acquisition. Phasor Analysis Precision: Accurately discriminate and quantify fluorescent species with unique spectral and lifetime features at every pixel. Dynamic Monitoring: Track multiple signaling events in live specimens with unprecedented clarity. Compatibility: Seamlessly integrates with Alba STED, Alba v5, Q2, PL1, Evident (Olympus), Nikon, and Zeiss systems. This innovation opens new doors for researchers studying dynamic biological processes, enabling discoveries that were once beyond reach. Find out more here: https://lnkd.in/gTbzKSSH Here’s to 40 years of pioneering excellence in fluorescence technology and to a future of continued breakthroughs! #ISS #FluorescenceImaging #SpectralFLIM #FastFLIM #PhasorAnalysis #BiomedicalResearch #MicroscopyInnovation #LifeSciences #ScientificAdvancements #40YearsOfISS

https://lnkd.in/g3r2kmgm Scientists at Max Planck Institute have developed a method based on MINIFLUX nanoscopy, an optical visualization technique similar to light or fluorescence microscope but at nanometer to angstrom optical resolution. By tagging biological macromolecules with different photo activable fluorescent markers the team was able to optically determine the spatial coordinates of 2 distinct points within a macromolecule and measure the distance between them down to 1nm resolution. This method has been developed as an alternative to FRET method for measuring intramolecular distances. #MINIFLUXnanoscopy #FRET #intramoleculardistances