JMSACL (Journal of Mass Spectrometry & Advances in the Clinical Lab)’s Post

📚💡Degradome analysis to identify direct protein substrates of small-molecule degraders #TPD #Targeted_Protein_Degradation

Chromatography Analysis of Endocannabinoids and Phytocannabinoids The endocannabinoid system (ECS) is made of a network of chemical signals and cellular receptors densely packed throughout our brains and bodies. Universidade Federal de Santa Catarina scientists recently looked at advancements made in the chromatographic analysis of endocannabinoids and phytocannabinoids. Link: https://lnkd.in/eF3YR6Qz

Breath Meets Silver for Better Untargeted Metabolomics! 🌬️🔬 A study conducted by Cedric Wüthrich, Renato Zenobi, and Stamatis Giannoukos from ETH Zurich considered Silver Nitrate (AgNO3) as an alternative dopant for untargeted breath metabolomics using (SESI-Orbitrap). This approach has revealed new aspects of detecting and analyzing biomarkers in exhaled breath. Results: ● Enhanced Ionization and Detection: AgNO3 improves ionization for compounds such as unsaturated hydrocarbons and sulfur-containing compounds, and reveals unique elemental ratios, particularly for low oxygen-carbon and high hydrogen-carbon ratios. ● Comprehensive Feature Coverage: AgNO3 provided the most comprehensive coverage of compounds in exhaled breath, identifying 1679 unique features. In comparison, aqueous FA detected 708 unique features, and NaI detected 531 unique features. ● High Molecular Mass Detection: Features detected using silver-doped electrospray possessed higher molecular masses and were more numerous compared to other conditions, leading to distinct separation in principal component analysis (PCA) plots. See full paper: https://lnkd.in/dTGAzQ9U #BreathAnalysis #Metabolomics #MassSpectrometry #Orbitrap #SESI #Science #Healthcare #ETHZurich #ResearchAndDevelopment

Targeted protein degradation Targeted protein degradation involves redirecting protein recycling systems in our cells to destroy the disease-causing proteins. Protein degraders work by capturing the disease-causing protein and making it stick like a glue to the cellular protein-recycling machinery, which then tags the protein as expired in order to destroy it. The tag is a small protein called ubiquitin, which effectively gets fired at the disease-causing protein like a bullet. In order for the process to work effectively, ubiquitin must hit the right spots on the target protein so that it gets tagged effectively. The new work by the team enables them to see how the bullet hits the proverbial bull’s eye. Working with a protein degrader molecule called MZ1, and using high-end mass spectrometry, they were able to identify exactly where on the target protein the vital ‘tags’ are added. The work shows how degrader drugs hold onto and position disease-causing proteins, making them good targets for receiving ubiquitin molecules (ie. "ubiquitin-atable") which then leads to their destruction inside the cell. #ScienceMission #sciencenewshighlights https://lnkd.in/gTFnHJUF

📚Advances in designing ternary complexes: Integrating in-silico and biochemical methods for PROTAC optimisation in target protein degradation #TPD #Targeted_Protein_Degradation

The health benefits of Baobab powder keep getting more exciting every day! A recent study using advanced Metabolomics identified 40 metabolites for the first time. The study also revealed the antidiabetic potential of Baobab with this abundance of bioactive phytochemicals. Histopathological and immunohistochemical studies demonstrated that Baobab exerted hepatoprotective and renoprotective effects in diabetic rats. Biochemical assays showed Baobab's capacity to lower the fasting glucose, lipid profile, and hepatic and renal markers. Baky MH, Badawy MT, Bakr AF, Hegazi NM, Abdellatif A, Farag MA. Metabolome-based profiling of African baobab fruit (Adansonia digitata L.) using a multiplex approach of MS and NMR techniques in relation to its biological activity. RSC Adv. 2021 Dec 13;11(63):39680-39695. doi: 10.1039/d1ra08277a. PMID: 35494142; PMCID: PMC9044842.  https://lnkd.in/gEju7zNr #Baobab #prebiotic #fiber #wellness #research #productdevelopment #naturalproducts #guthealth #superfood #sustainability #metabolicsyndrome

🚀 Exciting News! 🚀 We're thrilled to announce the launch of our new page dedicated to Bile Acid Analysis! 🌟 ✔ Discover more about the analytical challenges associated with bile acid analysis ✔ Gain a deeper understanding of Bile Acids and their important biological functions ✔ Explore cutting-edge techniques and innovative solutions designed to advance Bile Acid analysis by LC-MS/MS Find all our related content on this dedicated page: https://sciex.li/zdyuzp Dive into the world of metabolomics with us and discover how our tools can elevate your research to new heights. #Metabolomics #Lipidomics #MassSpectrometry #LifeScienceResearch #BiomedicalResearch #BileAcidAnalysis #Innovation #Targeted #Quantitation #Characterisation #SCIEX #ZenoTOF #OMICS

China: Exploring Metabolic Characteristics in Different Geographical Locations and Yields of Nicotiana tabacum L. Using Gas Chromatography-Mass Spectrometry Pseudotargeted Metabolomics Combined with Chemometrics

The goal of any nontargeted #metabolomic or #lipidomic study is to discover the most biologically important signals in a patient population, whether they are known molecules or yet to be mapped. Improvements in the speed, quality, and extent of #metabolite identification are needed to unlock the insights that unknown but statistically significant metabolites and lipids hold. We explore how technical advancements in discovery tools are now enabling efficient capture of multi-parameter data from thousands of chemical standards, supporting rapid molecule matching and maximizing the number of metabolites and lipids that can be identified to MSI Tier 1 reporting criteria: https://lnkd.in/d9BCqjpS #metaboliteidentification #metaboliteID #Tier1 #compoundID

Lately, I've been diving deep into the world of enzymes used in the clinical diagnostic laboratory, particularly proteinase K. This simple broad-spectrum serine protease acts as a tiny scissor-like enzyme, efficiently cutting proteins into smaller pieces by snipping the connections between their peptide bonds. In the molecular labs, it plays a crucial role in eliminating unwanted proteins and inactivating enzymes like RNase during nucleic acid extractions needed for downstream assays. Reflecting on the elegance of this molecular tool, a thought-provoking conundrum emerged – if proteinase K is a protein itself, why doesn't it denature itself? 🤔 #Enzymes #ProteinaseK #MolecularBiology #ResearchDiscussion