Jose Luis Torres Cuadros’ Post

🔬 Exciting Update! 🚀 Delve into my latest blog post exploring the game-changing potential of CRISPR technology in treating Sickle Cell Anemia. Join me as we uncover its transformative impact. Read more here https://lnkd.in/eVeSN7pV. #CRISPR #SickleCell #MedicalInnovation

𝗥𝗡𝗔 𝗲𝗱𝗶𝘁𝗶𝗻𝗴 𝗶𝘀 𝗴𝗮𝗶𝗻𝗶𝗻𝗴 𝘀𝗽𝗲𝗲𝗱, 𝗲𝘀𝗽𝗲𝗰𝗶𝗮𝗹𝗹𝘆 𝗳𝗼𝗿 #𝗿𝗮𝗿𝗲 𝗱𝗶𝘀𝗲𝗮𝘀𝗲 RNA editing is a new gene-editing technology that has entered clinical trials recently. It is believed to offer safer and more flexible gene-editing therapies. There are two RNA editing treatments currently being tested   💊 The first treatment by AIRNA focuses on RNA editing to make more precise medicines, using ADAR. The treatment replaces a single base to correct a mutation that leads to alpha-1 antitrypsin deficiency (#AATD). #AATD is a condition that can harm the lungs and liver.  https://lnkd.in/dN67F7AV    💊 The second treatment by Ascidian Therapeutics Therapeutics (Rewriting RNA) changes thousands of genetic letters in an RNA molecule simultaneously. Recently, FDA cleared the first Clinical Trial of #RNA Exon Editor Developed to Treat #Stargardt Disease, and other retinal diseases caused by mutations in the ATP binding cassette transporter gene, ABCA4. This treatment aims to treat the group of mutations that cause Stargardt disease. https://lnkd.in/d77AYwnk Kris Elverum , Michael Ehlers https://lnkd.in/d9z7sK4s

Aberrant non-canonical NF-κB signalling reprograms the epigenome landscape to drive oncogenic transcriptomes in multiple myeloma The downstream molecular mechanisms following the activation of the NF-κB pathway in multiple myeloma (MM) remain to be characterised. Here, it is shown that aberrant non-canonical NF-κB signalling causes epigenomic reprogramming leading to transcriptional changes that favour MM progression. https://lnkd.in/gUi3bYYP

DeepDoublet uses public PIC-seq, or pcRNAseq data to identify single cells that comprises doublets. It identified Angptl3 is expressed in hepatocytes when interacting with liver endothelial cells. Collaboration with Junil Kim's team and ByungKwan Lim's team. Project lead by Linbu Liao. https://lnkd.in/gbJqX6hH

Our last paper about Circular RNAs translation is out on: Nucleic Acids Reseach journal. Insights: 1. Circular RNAs can regulate transcriptions diversity 2. Intronic regions are necessary for circular RNA formation 3. Circular RNA are associated with polysomes 4. m6A methylation of circular RNAs promotes translation 5. Adenosine to Inosine (A>I) modification promotes circular RNA translation

Product Citation: StressMarq's Anti #Beclin-1 Antibody (SPC-600) was used in a new study examining mitochondrial #DNA deletions & cellular responses in the presence of oxidative phosphorylation complexes. SPC-600 🔎 https://bit.ly/3xkwZsp Paper 📖 https://bit.ly/4aiu1mI

Pleased to introduce MiCAA: a new way to analyze the Mitochondrial Chaperonin Activity Assay in live cells.

If you need a good read for the 🌧 weekend, check out our new preprint by Quezada 𝘦𝘵 𝘢𝘭. in which we began to fill a gap of knowledge by investigating the possible relationship between stress granules with #insulin #mRNA storage sites in beta cells, as a necessary premise to understand the potential implications of downregulated gene expression of G3BP1 in islets of subjects with #T2D. 👉 https://lnkd.in/eUPxPfTE

In case you missed them, BioIVT's scientific #posters and #whitepaper from #ASGCT2024 are now available for download! We explore the critical relationship between #temperature, #time, and the #stability of immune cell populations in #leukopaks so you can maximize the potential of #PBMCs in your breakthrough #CellandGeneTherapy research. It features an in-depth look at cell #viability following fresh #leukapheresis under different simulated shipping conditions for indispensable insights that could redefine your approach to cell transport and preservation. Our other poster evaluates #ASGPR expression and donor dependent cellular uptake and knockdown efficiency of a N-Acetylgalactosamine (GalNAc)-conjugated siRNA in different #hepatocyte models. This poster provides useful data for the selection of appropriate #invitro test systems for those researching new therapeutic modalities, including #oligonucleotides, to combat #liverdisease using the additional site selectivity that can be achieved using GalNAc conjugated to therapeutic RNA to direct liver delivery. Download the posters and whitepaper here: https://hubs.ly/Q02wX4cz0 #CellTherapy #GeneTherapy #ADME #Biospecimens

Genome-wide screening identifies Trim33 as an essential regulator of dendritic cell differentiation :- •Dendritic cells (#DCs) differentiate from bone marrow progenitors through a process guided by the cytokine Flt3 ligand (Flt3L). •Using CRISPR-Cas9–based screening, Tiniakou et al. identified key regulators of murine Flt3L-driven DC differentiation including the arginine methyltransferase Carm1, mTOR-negative regulators TSC and GATOR1, and the transcriptional repressor Trim33. •Trim33-deficient mice displayed a near-complete loss of multiple DC subsets with minimal impact on monocytes or granulocytes. •During DC differentiation, Trim33 repressed expression of type I interferon and genes associated with other myeloid lineages. These findings identify Trim33 as a key transcriptional regulator of the Flt3L-driven pathway of DC differentiation.