Joseph Odongo’s Post

Revolutionizing DNA Amplification and PCR Experimentation Polymerase GPT has completely transformed the way I approach DNA amplification and PCR experimentation. As a molecular biologist, I often found myself bogged down by the trial-and-error process of primer design, determining ideal PCR conditions, and analyzing electrophoresis results. Polymerase GPT has made this entire workflow not only faster but also significantly more reliable. With Polymerase GPT, I can easily determine DNA amplification base pairs on electrophoresis, making sense of band patterns that used to take me hours to interpret. The tool even goes a step further, suggesting ideal PCR conditions based on the target sequence and anticipated challenges, which has boosted my amplification success rate to new heights. Its ability to design primers accurately and quickly is unparalleled. Rather than manually designing and cross-referencing primers for specificity, Polymerase GPT provides optimal suggestions almost instantaneously. Additionally, it retrieves relevant references, supporting the suggested PCR conditions and primer designs, giving me confidence in the process. The integration of detailed electrophoresis analysis, along with references for best practices, makes Polymerase GPT an indispensable resource for my laboratory work. Simply put, Polymerase GPT has helped me overcome some of the most time-consuming bottlenecks in PCR workflows, allowing me to focus on discovery rather than troubleshooting. It's a must-have tool for anyone involved in genetic research or molecular biology. https://lnkd.in/dD5zVe2A

Superbio DNA Magnetic Purification Kit Catalog No. A00115-100, 100 Reactions/Box  This kit utilizes magnetic bead extraction technology, serving as an efficient and rapid DNA extraction tool designed specifically for obtaining high-quality DNA from trace samples such as droplets, sweat stains, and shed cells.  Product Advantages: 1. Wide Applicability: Effectively extracts DNA from various types of trace samples, including nails, bloodstains, hair, shed cells, and saliva stains.    2. Fast and Efficient: Simple experimental procedure; high-purity DNA can be extracted in a short time (70 minutes).    3. Convenient and Safe: Does not require the use of phenol and chloroform reagents; free from highly toxic substances.    4. Excellent Extraction Efficiency: Extracts high-quality DNA from very small sample amounts, ensuring good STR balance. Extracted samples can be directly used in subsequent molecular experiments such as PCR, sequencing, restriction enzyme digestion, cloning, and library construction.

https://lnkd.in/d6kTMQm8 🟢 Proteins provide essential functions in all biological steps. They are catalyst agents in biochemistry reactions, carry and store other molecules, provide signal transduction, are essential constituents in the structure of various biologics organisms, etc. Thus, reliably unraveling the biological process of proteins in the cell is the path to the solution and understanding of various diseases, suggesting advances in chemistry, pharmacy, biology, biochemistry, medicine, and economic development. 🟡However, big molecules have many possibilities to change conformation due to the free rotation of any sigma bond. They show a complex nature driving several intra- and intermolecular interaction possibilities and many structural conformations. A proteome concerns the set of all proteins expressed in a cell, tissue, or organism, from the functional expression of a specific genome. Thus, the term proteomics encompasses strategies for the identification and quantification of all proteins from a specific proteome. The proteome is a dynamic system in which each protein has interconnections in the cell that can have many different properties (chemical, physical, and biological dimensions). When analyzed altogether, it describes the phenotype of the cell/organism. 🔴 Proteomic analysis is complex, time-consuming, and expensive. To solve the proteome’s biomolecular constituents before detection and identification is essential to use high separation power techniques. Currently, multidimensional liquid chromatography (LC×LC) has the most appropriate attributes for that. Also, a molecular detection technique for accurate characterization is essential. Thus, mass spectrometry is a crucial tool because of its versatility. Besides, this technique provides an incredible separation power, separating ions by a difference as low as 0.0001 m/z in high-resolution instruments. Such versatility of MS adds to the separation power of theLC×LC. Therefore, in terms of high orthogonality of separation, the state of the art in proteomic studies is multidimensional liquid chromatography coupled with high-resolution tandem mass spectrometry. 💻 In this video, you can see a summary in animation explanation of how works an analytical method to proteomics analysis. REFERENCE: The Importance and Challenges for Analytical Chemistry in Proteomics Analysis. Available from: https://lnkd.in/eKp5JpS... [accessed Feb 26 2024]. For more details of the method, see the thesis: Exploring the fundamentals of liquid chromatography and mass spectrometry for integration between proteomics, microfluidics and chemometrics Thesis available at the University of Sao Paulo (USP) Digital Library: https://lnkd.in/dsek8tjs Sao Carlos Institute of Chemistry University of Sao Paulo Grant #2015/16025-8 and 2017/17368-1 , São Paulo Research Foundation (FAPESP).

Our work on RNA polymerase recycling is finally published!!! Read to learn about how structural and mechanical effects regulate transcription recycling.

EUROArray is a technique that combines PCR and microarray technologies, using BIOCHIPs to detect up to 36 DNA sequences at once. It allows for quick and accurate testing of multiple samples simultaneously. To learn more about our molecular portfolio, visit https://lnkd.in/e8wmpcdK #microarray #molecular #diagnostics

Google just released AlphaFold 3 to public domain, inducing democratization of biochemistry. You still need to use strong hardware and get Google approval, but now you can predict your own protein structures including but not limited to: 👉🏽 Model large biomolecules such as proteins, DNA, and RNA. 👉🏽 Predict the structure of small molecules known as ligands, which encompasses many drugs. 👉🏽 Model chemical modifications to these molecules, which are important for cellular function. 👉🏽 Generate joint 3D structures of molecules, showing how various biomolecules interact within a cellular context. 👉🏽 Predict complexes containing "nearly all molecular types present in the Protein Data Bank". https://lnkd.in/eB8-kZr3 #alphfold #publicdomain #chemicalprediction

Smart-Q Fluorometer, for quantification of DNA, RNA and protein samples Sensitive quantitation down to 0.1pg/ul ds DNA Large color touch screen for programming and operation Two models for single or 8-sample processing Built-in reagent calculators for sample preparation Compact and portable (11 x 6 inch footprint)

PCR cycles explaned PCR - polymerase chain reaction: a technique for rapidly producing many copies of a fragment of DNA for diagnostic or research purposes Youtube video: https://lnkd.in/d_csejyg #nikolaysgeneticslessons

𝐐𝐅𝐀𝐒𝐆, an automated method developed to construct ligands for a target protein from a library of molecular fragments.Quantum-assisted Fragment-based Automated Structure Generator (QFASG) was applied to generate new uM inhibitors of CAMKK2 and ATMPaper: https://lnkd.in/dbVyu5dz

Remembering PCR steps: @El-Sayed Mahgoub Process of the polymerase chain reaction (PCR)‼️ Overview The process of the polymerase chain reaction involves manipulating temperatures to cause denaturation and annealing in a four-step process. The polymerase chain reaction requires the following materials to take place: • a DNA sample that subsequently gets denatured and amplified through the polymerase chain reaction • Taq polymerase is required in the elongation stage to bind complementary nucleotides to the single-stranded DNA • nucleotide bases must be constantly available for Taq polymerase to create a new strand that is complementary to the single-stranded DNA • sequence-specific DNA primers join to the 3’ end of single-stranded DNA by complementary base pairing to form the first segment of double-stranded DNA, allowing Taq polymerase to attach and begin extending the DNA strand. To begin the polymerase chain reaction, a mixture of the above materials is placed into a thermal cycler, where it undergoes the following processes: 1 Denaturation – DNA is heated to approximately 90–95 °C to break the hydrogen bonds between the bases and separate the strands, forming single-stranded DNA. 2 Annealing – the single-stranded DNA is cooled to approximately 50–55 °C to allow the primers to bind to complementary sequences on the single-stranded DNA. 3 Elongation – the DNA is heated again to 72 °C, which allows Taq polymerase to work optimally. Taq polymerase binds to the primer, which acts as a starting point, and begins synthesising a new complementary strand of DNA. 4 Repeat – the cycle (steps 1–3) is repeated multiple times to create more copies of DNA 🧬