PROMISE Proteomics’ Post

AD | Unleash the power of proteomics in your lab! Speak to an expert now to learn how the Platinum® Next-Generation Protein Sequencer™ can help you achieve your current research goals! Our latest V2 Sequencing Kits bring together enhanced proteome coverage and improved sequencing output seamlessly, offering unparalleled simplicity and exciting opportunities for your research. The new Platinum system offers application capabilities, such as: • Protein Barcoding: Utilize peptide barcodes to directly sequence proteins with single amino acid resolution.  • Protein Variants: Rapid and accurate distinction of variants based on differences in the amino acid sequences.  • Post-Translational Modifications (PTMs): Discover the power of using Kinetic Signatures to sequence proteins and detect changes in the proteome, including PTM analysis. Contact Us: https://bit.ly/4aFyOP4

Imagine analyzing both #metabolites and #proteins from a single sample, with enhanced sensitivity and resolution. That's the promise of PANAMA (proteomic and nanoflow metabolomic analysis), a novel workflow described in a recent publication. By combining solid-phase micro-extraction with nanoflow LC-MS, PANAMA delivers comprehensive, high-quality data, opening new doors for research in various fields. Here’s the full article: https://lnkd.in/ewcUjNDh A big shoutout to the authors for their work! Weiwei Lin, Fatemeh Mousavi, Ph.D., Benjamin B., Christian F Heckendorf, Matthew Lawton, Noah Lampl, Ryan Hekman, Hongbo Guo, Mark McComb, Andrew Emili. #metabolomics #proteomics #LCMS #nanoflow #research #LC #liquidchromatography #nLC #analyticalchemistry

🔬 New Protocol Alert! Protocol for unified metabolomics and proteomics analysis of formalin-fixed paraffin-embedded tissue By Isaiah et al. from Shayne Mason’s lab from North-West University / Noordwes-Universiteit Summary: The use of archival formalin-fixed paraffin-embedded (FFPE) tissue samples for biochemical analyses is problematic because of the formation of a Schiff base, leading to low protein and metabolite yields during analytical extractions. Highlights: Using 20 mg of wet mass tissue, this protocol consistently extracted more than 50 metabolites (across 11 classes of metabolites) and over 900 proteins. Read the full protocol here: https://lnkd.in/dEDTKmsG #STARProtocols #CellPress #metabolomics #proteomics #FFPEtissue

Comparing iTRAQ, TMT, and SILAC: Which Quantification Technique Works Best for Your Proteomics Research? When it comes to quantitative proteomics, choosing the right technique is key to reliable results. In our latest infographic, we break down the differences between three popular label-based quantification methods: iTRAQ, TMT, and SILAC. Whether you're working in vitro or in vivo, each technique has unique advantages: iTRAQ: Known for its 4-plex and 8-plex reagents, it's a go-to for peptide and protein labeling. TMT: With up to 11 tags, TMT offers higher multiplexing capabilities. SILAC: This in vivo method uses isotopically labeled amino acids to quantify proteins directly in living cells. Ready to dive deeper into their principles, workflows, and characteristics? Download our detailed PDF to explore how each can elevate your research. 🔗 Download the PDF here: https://lnkd.in/eW9jWwFn #Proteomics #iTRAQ #TMT #SILAC #QuantitativeProteomics

Curious about how advanced mass spectrometry tools can change the game in protein analysis? 🧬 Dive into our latest comparison: Fusion Lumos vs. TimsTOF Pro2 for label-free protein quantification in saliva samples. 📊 Find out which system excels in sensitivity, speed, and coverage. Explore how each can transform complex sample analysis in the bio-lab setting. Ready to see the future of proteomics? Let's explore together! 👇#Proteomics #MassSpec #BioTech #ProteinAnalysis #DataScience #ResearchInnovation https://lnkd.in/gtm5rNk9

Fully functionalized probes are designed to speed up and simplify the early stages of drug development. The introduction of the diazirine photocrosslinking moiety along with the presence of a functional acetylene group allows the screening of compounds directly in cells. In recent work, Offensperger et al. used a chemical proteomics approach to map protein-ligand interactions across the human proteome. With a library of around 400 small-molecule fragments from #Enamine ’s catalog (harboring a diazirine moiety for photocrosslinking and an alkyne functionality for click reaction and enrichment), authors integrated machine learning to predict interactions within biological systems (https://lnkd.in/dd9Tvs8T). Enamine currently has almost 8 000 fully functionalized compounds in stock and more than 1 million molecules in REAL Database. The most diverse compounds have been selected and sorted out into a small library, which has been pre-plated for the most convenient and fast delivery to our clients. For more details about Enamine Fully Functionalized Probe Library https://lnkd.in/dkwpPsij

What is the connection between Olink Proteomics and Shakespeare? The Olink Explore HT assay from Olink Proteomics allows for comprehensive proteomic screening of microsamples of serum or plasma. In fact, from a single 2 μl sample, we can analyse concentrations of more than 5400 proteins. A complete Olink Explore HT Panel is a streamlined parallel assay of 172 samples, providing a total of more than 900,000 data points of protein concentrations. To put that number into perspective, this exceeds the total number of words in the complete works of William Shakespeare, including all his 38 plays and 154 sonnets. All this from 344 μl of plasma or serum (or from 0.1 fluid drachm, the unit used for measuring small volumes of liquid in Shakespearean England). Olink Explore HT is a next-generation solution for high-throughput proteomics with remarkable specificity and an automated workflow. The Explore family of assays has been instrumental in some of the most notable population-scale proteomic studies, such as the UK Biobank Pharma Proteomics Project, where over 54,000 participants had their genomes and proteomes analysed. As a certified Olink service partner since 2017, BioXpedia has the experience to help you design, conduct, and analyse Olink Explore HT studies. Contact us for an informal discussion about your study ideas. #ClinicalResearch #LifeScience #Biomarkers #Precisionmedicine #Proteomics #MedicalSciences

Introducing Our Latest Data Sheet: Sequence Proteins with Precision Using Platinum® and our Sequencing Kit! Platinum, the world’s first Next-Generation Protein Sequencer™, delivers single-molecule and single-amino acid resolution in a user-friendly benchtop platform. Platinum enables protein identification and variant detection without complex workflows and advanced expertise, making proteomics accessible for every lab. Platinum and Sequencing Kit V3 enable new protein identification and characterization applications with enhanced sequencing performance and new analysis methods for studying unknown proteins. Download the Data Sheet to learn more about how Platinum is advancing protein research. https://lnkd.in/eiFEB3VG #Proteomics #NextGenSequencing #ProteinSequencing #LifeSciences

Looking to outsource some of your protein biomarker assay work....look no further. BioXpedia A/S can help by using the Olink Explore HT assay for comprehensive screening of microsamples of serum or plasma. #biomarkers #proteomics #clinicalresearch

Here Jiang et al characteristics the proteolytic preformed of the following proteases :proteinase K, subtilisin, and thermolysin. With emphasis of coast per run and speed of digestion 🎯 Summary: Jiang et al observed that all three enzymes could produce a digest with in 1-5 minutes Futhermore the cost was significantly low tjat traditional proteomics-grade trypsin by an order of 200-100] fold! Jiang et al where able in their hands to get comparable results to trypsin with theese novel proteases. One note of caution is that Jiang et al where not able identify distinct cleavage patterns alone. The use of machine learning could distinguish true protease products from random cleavages. #massspec #massspectrometry #biopharmaceuticals #chemistry #protomics #biotech #science #scienceandtechnology #sciencecommunication #innovation https://lnkd.in/evZYVUb4