A researcher of plant sciencesll plants Genetics ll weed ecology ll plant physiology. Dedication towards plants microbes interaction ll Physiology Genetic Engineering ll plants Breeding ll crop management.
Summary of the CRISPR/Cas9 based steps of plant genome editing. In the first step identification of best target sites is done using various online tools and primers are designed. The target-specific sgRNA with Cas9 cassettes are cloned in binary vector. In next step cassettes are then co-transformed in vivo into the plants. The transgenic plants are confirmed for the target-specific mutations using various approaches like high-throughput sequencing approaches. The positive transformed plant is then selected for further applications and analysis.
