Nova One Advisor’s Post

Whole genome amplification (WGA) is a key step in DNA sequencing from low-input DNA samples, such as forensics materials, cell-free DNA from blood, and spent media from cell culture. Commonly used methods, such as DOP-PCR, MDA, MALBAC, and LIANTI, all have drawbacks such as amplification bias, poor uniformity, errors and artifacts, low genome coverage, inability to address all variant classes, low accuracy, poor reproducibility, and/or complex protocols that are difficult to automate or scale. Primary template-directed amplification (PTA) is a novel, isothermal WGA method that reproducibly captures >95% of the genomes of single cells, in a controlled and more uniform and accurate manner than existing approaches. This improves variant calling sensitivity and specificity, lowers sequencing costs, and facilitates bioinformatic analysis. - PTA enables superior genomic coverage versus other single-cell whole genome methods and is comparable to bulk sequencing - PTA enables superior single nucleotide variant (SNV) detection versus other single-cell whole genome methods and is comparable to bulk sequencing - PTA enables superior copy number variation (CNV) detection versus other single-cell whole genome methods and is comparable to bulk sequencing Get more from your DNA sequencing – request a quote for our ResolveDNA WGA Kits today! ⤵ https://lnkd.in/g-XptHu7

Will short-read sequencing become obsolete? Short-read sequencing is a DNA sequencing method that generates short fragments of DNA (typically 50-300 base pairs). On the other hand, Long-read sequencing (such as PacBio or Nanopore sequencing) is a genomic technology that produces lengthy DNA or RNA sequences, often exceeding thousands of base pairs. Short-read sequencing is known for high-throughput genome analysis and cost-effectiveness, making it perfect for applications such as WGS, WES, or single-gene testing. However, it struggles with assembling complex genomic regions such as repetitive sequences and structural variants. Moreover, challenges arise in resolving long, repetitive regions, making it unsuitable for applications such as de novo genome assembly. In contrast, long-read sequencing excels at identifying structural variants, large insertions and deletions, and resolving repetitive regions. However, a major drawback of such techniques is their price, which is often much higher than for short-read sequencing. In the longer run, long-read sequencing may become cost-efficient. This ultimately raises the question: Will short-read sequencing become obsolete?

Pioneering Genomic Progress: An Interview with Rami Mehio, Illumina's Software and Informatics Expert In this interview, Rami Mehio, the head of software and informatics at Illumina, talks about his experiences and contributions to significant genomic projects, such as the whole genome sequencing for the UK Biobank. He explores the challenges and innovations in analyzing genomic data, emphasizing how Illumina plays a crucial role in pushing forward genetic research and precision medicine.   https://lnkd.in/dX_vraCz

Let's learn about Next Generation Sequencing Nick McCooke led the pioneer team at Solexa that invented next-generation sequencing, a technology to read DNA at high speed that is nowadays used worldwide and has laid the foundation for precision medicine. Solexa was acquired by Illumina in 2006 for what amounted to around €500M back then. Next generation sequencing (NGS), massively parallel or deep sequencing are related terms that describe a DNA sequencing technology which has revolutionised genomic research. Using NGS an entire human genome can be sequenced within a single day. Understanding the basic steps in the NGS workflow 1. Step 1: Nucleic acid extraction. The overall workflow for an NGS experiment starts with the isolation of genetic material. 2. Step 2: Library preparation. 3. Step 3: Sequencing. 4. Step 4: Data analysis and interpretation. Next-generation sequencing (NGS) is a massively parallel sequencing technology that offers ultra-high throughput, scalability, and speed. The technology is used to determine the order of nucleotides in entire genomes or targeted regions of DNA or RNA. Thanks and Best Regards Sri Manoj Kumar Head of Biomedourse Atheenapandian Subsidiary of  ATHEENAPANDIAN - India's No.1 Biomedical Industry for Training & Placements PH. +918807039891 WWW.ATHEENAPANDIAN.COM

If you are interested in improving your NGS data for clinically relevant samples, check out this webinar sponsored by SciLifeLab and presented by Watchmaker Genomics CSO, Brian Kudlow

NimaGen prides ourselves on being "Innovators in DNA Sequencing Technologies'', so we would like to wish everyone a Happy DNA Day! What is DNA day and why are we commemorating it? DNA day was officially recognized by the U.S. on April 25th and celebrates anually the discovery of the double-helix structure of DNA by Watson and Crick in 1953, as well as the completion of the Human Genome project in which the first human genome was completely sequenced in 2003. This day offers educators, students, scientists and the general public a day to reflect and learn about advances in DNA sequencing technology. Do you know about our proprietary core technology used in our NGS/MPS Library Prep Kits known as Reverse Complement PCR (RC-PCR)? Reverse Complement PCR (RC-PCR) is a modification of the polymerase chain reaction (PCR). It is primarily used to generate amplicon libraries for DNA sequencing by Next-generation Sequencing (NGS). This patented and unique technique permits the simultaneous multiplex amplification, adapter ligation and indexing in a single closed tube reaction.    In RC-PCR, no target specific primers are present in the reaction mixture. Instead target specific primers are formed as the reaction proceeds. A typical reaction employing RC-PCR requires four oligonucleotides, that interact with eachother in pairs: one RC-PCR probe, one universal primer (containing functional domains of choice), which hybridize with each other at their 3’ ends. The RC-PCR probe contains the reverse complement sequence of the desired target specific primer sequence. Once RC-PCR probe and universal primer have hybridized, the universal primer can be extended, using the oligonucleotide probe as the template, to yield fully formed, target-specific primers, which are then available to amplify the target DNA in subsequent rounds of thermal cycling like in a standard PCR reaction. Learn more here: https://lnkd.in/dtRCQUJT

Next-generation sequencing (NGS) technologies are advancing the field of genomics, enabling rapid analysis of DNA and RNA sequences. Multiple studies show these cutting-edge tools are revolutionizing how health care providers decipher the genetic makeup of diseases and tailor treatments to individual patients. The implications are profound, promising a future where medical care is more precise and effective. https://lnkd.in/g7hNn5Np

'Element secures a substantial $277 million in funding to enhance its position in the sequencing market, setting the stage for intense competition with Illumina. While Illumina has traditionally dominated short-read sequencing, emerging players like Pacific Biosciences and Oxford Nanopore Technologies are making strides in long-read sequencing. Short-read DNA sequencing excels at capturing most genetic variations, whereas long-read sequencing proves superior in detecting intricate structural variants. #Genomics #SequencingMarket

PacBio Revio long reads HiFi - Tomorrow’s genomic discoveries start today Accuracy of 99.9% (0.1% error rate) for 15–20 kb reads with the added benefit of length needed to span genomic repeats. Sequencing costs like popular mid-throughput platforms for short reads but the information provided by HiFi sequencing is richer, delivering a lower cost-per-answer After sequencing, fast PacBio analysis solutions are available for every step of the workflow, from variant calling to phased de novo genome assembly. Tools like the PacBio WGS Variant Pipeline are available for secondary and tertiary analysis, from alignment to annotation Revio system allows you to simultaneously run 4 SMRT Cells in a single sequencing run, meaning scientists can utilize one cell for WGS, one cell for RNA, one cell for single-cell RNA, and one cell for amplicons — all at once, with methylation calling in every sequencing run https://lnkd.in/durHMNM8