🎓 Scientific Highlight of the Week 🎓 We are delighted to share insights from a groundbreaking study entitled "Arf1-dependent LRBA recruitment to Rab4 endosomes is required for endolysosome homeostasis" published in Journal of Cell Biology by Viktória Szentgyorgyi et al: https://lnkd.in/dw9Xvazn Congratulations to all authors for this great article. They showed that lipopolysaccharide responsive beige-like anchor protein is recruited by members of the small GTPase Arf protein family to the TGN and to Rab4+ endosomes, where it controls intracellular traffic. In patient-derived fibroblasts, loss of LRBA led to defects in the endosomal pathway promoting the accumulation of enlarged endolysosomes and lysosome secretion. Our Helix-IN transfection reagent was used to transfect HeLa cells to generate CRISPR-Cas9 KO cell line. Viktória Szentgyörgyi, Leon Lück, Daan Overwijn, Danilo Ritz, Nadja Zoeller, Alex Schmidt, Maria Hondele, Anne Spang, Shahrzad Bakhtiar #transfection, #Hela, #crispr, #cas9, #geneediting, #endosomes, #metabolism, #severeimmunedysregulation, #lipopolysaccharide, #ozbiosciences
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Project Fellow@ DVIKAR@Gujarat University | PhD in Medicine (Cambridge University, UK)
New insights into bacterial vaginosis (BV) causes have been discovered! Recent research has shown that disruptions in the vaginal microbial equilibrium can lead to BV. The study utilized 16S rRNA sequence analysis to investigate microbial composition variations between control and BV samples. Potential therapeutic targets and their corresponding inhibitory ligands were identified and further confirmed through molecular docking and MD simulations. The study also detected several anaerobic bacteria known for their ability to generate biofilms. Excitingly, we identified Dalfopristin, Clorgyline, and Hydrazine as potential therapeutic options for the management of BV. Read the full article to learn more about these novel pharmacological treatments. #BV #microbialcomposition #therapeutictargets #noveltreatments
16S rRNA female reproductive microbiome investigation reveals Dalfopristin, Clorgyline, and Hydrazine as potential therapeutics for the treatment of bacterial vaginosis
sciencedirect.com
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🌟 New Insights into Cellular Dormancy and Ribosomal Hibernation! 🌟 Dormant #cells enter a non-proliferative state with low metabolic activity, sequestering ribosomes as dormant/hibernating ribosomes. These #ribosomes are crucial for ribosome preservation and #translation shut-off. Despite recent studies, the regulation and roles of diverse dormant ribosomal populations are still largely unknown. The review of Koli et al. https://zurl.co/XyEu explores the formation, regulation, and disassembly of dormant ribosomes during stress and recovery, highlighting key unanswered questions in ribosomal dormancy research. Immagina’s technologies RiboLace and AHARIBO can also be used to study ribosome heterogeneity, make sure to visit https://zurl.co/lcQY to learn more! 📚 #CellBiology #RibosomeResearch #Dormancy #Hibernation #TranslationRegulation
Ribosomal dormancy at the nexus of ribosome homeostasis and protein synthesis
onlinelibrary.wiley.com
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This brown fat thing and GIP - there is other things going on GIP increases oxidation of brown fat - brown fat apparently does other things when oxidized. Muojario a dual GIP GLP-1 medication really has weight loss side effects . 2021 Nov;45(6):840-852. doi: 10.4093/dmj.2020.0291. Epub 2021 Jun 25. Brown Fat as a Regulator of Systemic Metabolism beyond Thermogenesis Okamatsu-Ogura Yuko 1, Masayuki Saito 1 2 Affiliations expand PMID: 34176254 PMCID: PMC8640153 DOI: 10.4093/dmj.2020.0291 Abstract Brown adipose tissue (BAT) is a specialized tissue for nonshivering thermogenesis to dissipate energy as heat. Although BAT research has long been limited mostly in small rodents, the rediscovery of metabolically active BAT in adult humans has dramatically promoted the translational studies on BAT in health and diseases. Moreover, several remarkable advancements have been made in brown fat biology over the past decade: The molecular and functional analyses of inducible thermogenic adipocytes (socalled beige adipocytes) arising from a developmentally different lineage from classical brown adipocytes have been accelerated. In addition to a well-established thermogenic activity of uncoupling protein 1 (UCP1), several alternative thermogenic mechanisms have been discovered, particularly in beige adipocytes. It has become clear that BAT influences other peripheral tissues and controls their functions and systemic homeostasis of energy and metabolic substrates, suggesting BAT as a metabolic regulator, other than for thermogenesis. This notion is supported by discovering that various paracrine and endocrine factors are secreted from BAT. We review the current understanding of BAT pathophysiology, particularly focusing on its role as a metabolic regulator in small rodents and also in humans.
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Part 2: Method: We used exposure to a bacterial lipopolysaccharide (LPS) to induce neonatal endotoxemia in the mice. Results: It was found that NGN (100 mg/kg i.p.) exposure during the neonatal period reduced leukocyte migration and decreased pro-inflammatory cytokine (TNF-α, IL-1β and IL-6) levels in the lungs, heart, kidneys or cerebral cortex. In addition, NGN upregulated IL-10 production in the lungs and kidneys of neonate mice. The administration of NGN also enhanced antioxidant enzyme catalase and SOD activity, reduced lipid peroxidation and protein carbonylation and increased the reduced sulfhydryl groups in an organ-dependent manner, attenuating the oxidative damage caused by LPS exposure. NGN decreased ERK1/2, p38MAPK and COX-2 activation in the lungs of neonate mice. Moreover, NGN complexed with HPβCD was able to increase the animal survival rate. Conclusion: NGN attenuated inflammatory and oxidative damage in the lungs, heart and kidneys caused by neonatal endotoxemia through the MAPK signaling pathways regulation. Our results show that NGN has beneficial effects against neonatal endotoxemia and could be useful in the treatment of neonatal inflammatory injuries. Heimfarth L, Int J Biol Macromol. 2024 Feb 28:130500. doi: 10.1016/j.ijbiomac.2024.130500. Epub ahead of print. PMID: 38428770. #Gesundheit #Bildung #Fuehrung #Coaching #Mindset #Motivation #Gehirn #Neuroscience #Psychologie #Persoenlichkeitsentwicklung #Kindheit #KeyNoteSpeaker #Humangenetik #Biochemie #Neuroleadership #Ernaehrung #Transformation #Stress #Demografie #Gender #Age #interkulturelleKompetenz #Epigenetik #Veraenderung #EmotionaleIntelligenz #Change #Gesellschaft #Organisationsentwicklung #Philosophie #Beratung # Quantum
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#Genetic assessment of the causal effect of plasma metabolites and metabolic pathways on #delirium https://lnkd.in/g7B_S4Cb
Genetic assessment of the causal effect of plasma metabolites and metabolic pathways on delirium - Anesthesiology and Perioperative Science
link.springer.com
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📢 Review Sharing-Vol. 29 No. 2 💕 Title: Recent Advancements in the Atopic Dermatitis Mechanism 🤵 Authors: Maria Savva*, Nikolaos G Papadopoulos, Stamatis Gregoriou, Spyridoula Katsarou, Niki Papapostolou, Michael Makris, Paraskevi Xepapadaki1 🔔 Full Text: https://lnkd.in/giAyt_9d 🔑 Keywords: atopic dermatitis; atopic eczema; pathophysiology; skin barrier disruption; immunopathogenesis; skin microbiome; exposome; endotypes 😎Welcome to your reading! #Bioscience #biomedicalscience #biochem #medicalscience #ScienceCommunication #Biochemistry #StemCell #Virology #CancerResearch #immunology; #Genetics #MolecularBiology #Microbiology #medicine #health
Recent Advancements in the Atopic Dermatitis Mechanism
imrpress.com
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We are pleased to share with you our last thoughts about mitochondrial diseases and tissue specificity in our recent publication in Trends in Endocrinology & Metabolism (Cell Press). Find below a free link to the article! Enjoy it! https://lnkd.in/dVRePZf5
ISR pathway contribution to tissue specificity of mitochondrial diseases
sciencedirect.com
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🔬 Exploring early signals of metabolic derangements in Nonalcoholic Fatty Liver Disease (NAFLD) with Impaired Fasting Glucose (IFG) using plasma exosomal metabolomics. 🧪🫁 Preliminary findings reveal distinct changes in fatty acids, paving the way for further exosome biology investigation. https://lnkd.in/dzpbGaND #TurkishJournalofGastroenterology #TJG #GastroenterologyResearch #MedicalJournals #GastrointestinalResearch #February #NewIssue #NAFLD #Metabolomics #MedicalResearch
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https://lnkd.in/gn-DEBGx Article title: Protein S100A8/A9: A Potential New Biomarker for Pancreatic Diseases Author(s): Alexander T El Gammal; Jörn H Sturm; Hans O Pinnschmidt; Bianca T Hofmann; Eugen Bellon; Tarik Ghadban; Nathaniel Melling; Kai Bachmann; Jakob R Izbicki; Maximilian Bockhorn; Cenap Güngör; Daniel R Perez Journal: International Journal of Clinical Endocrinology and Metabolism Journal ISSN: 2640-7582 Abstract: Objectives: S100A8/A9 expression has been linked to carcinogenesis and inflammation. We hypothesized that S100A8/A9 protein serum levels are a useful stratification marker for patients with pancreatic ductal adenocarcinoma (PDAC), intraductal papillary mucinous neoplasm (IPMN) or chronic pancreatitis (CP). #EndocrineGlands #Hormones #HormoneMetabolism #StructureAndPhysiochemicalProperties #HormonalReceptors #SignalingMechanisms #HormoneRegulatedGeneExpression #IntracellularSteroidAndLipidMetabolism #BoneAndMineralMetabolism #Infertility #Peertechz #PeertechzPublications #SexualDifferentiation #Puberty #PediatricEndocrinology #EndocrinePharmacology #MolecularEndocrinology #GastrointestinalEndocrinology #Neuroendocrinology #ComparativeEndocrinology #CardiovascularEndocrinology #ReproductiveEndocrinology
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