PHASEVIEW’s Post

How to embed a small specimen in a well of low melting agarose and Phytagel and mount it on a stage for imaging ?  The architecture of PHASEVIEW Alpha3 light sheet microscope using a fluorescence microscope stand as a detection unit allows it to adapt to any experiment setting. Follow these steps : - Use T-spike holder  - Put forming mold on top of holder - Fill with liquid - 0,8 % phytagel - Insert well-shaping mold  - Wait for phytagel to solidify - Remove shaping molds - Add specimen and medium to well - Close with low-melting agarose drop.  - Insert mold into sample chamber - Adjust sample orientation  - Place sample chamber on translating stage More information in this tutorial :

To view or add a comment, sign in

Explore topics