How to embed a small specimen in a well of low melting agarose and Phytagel and mount it on a stage for imaging ? The architecture of PHASEVIEW Alpha3 light sheet microscope using a fluorescence microscope stand as a detection unit allows it to adapt to any experiment setting. Follow these steps : - Use T-spike holder - Put forming mold on top of holder - Fill with liquid - 0,8 % phytagel - Insert well-shaping mold - Wait for phytagel to solidify - Remove shaping molds - Add specimen and medium to well - Close with low-melting agarose drop. - Insert mold into sample chamber - Adjust sample orientation - Place sample chamber on translating stage More information in this tutorial :
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GAOTek Achromatic Optical Fiber Microscopes This achromatic optical fiber microscopes has 7 in screen, 1.18 in to 2.75 in coarse adjustment range, and 4.33 in × 4.72 in working stage.
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My new published article. DOI 10.1088/2040-8986/ad77e3 A widefield microscope based on a Linnik interferometer was designed, constructed, and tested. The phase-shifting and polarized single-shot methods were used to measure interference patterns. Both methods use a low-coherence light-emitting diode as the light source, achieving a resolution of 10 nm in the Z direction and diffraction-limited resolution in the X and Y directions. The single-shot method is vibration-insensitive, allowing for the observation of moving objects. The simplicity and low cost of this instrument make it valuable for a wide range of applications.
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Here is an example of Poiseuille flow for a multi-mode Giesekus model and a logarithmic formulation. The velocity field is not disturbed, and the constraints are not disturbed in the flow. The variables are dimensionless. 😊
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𝗬𝗼𝘂 𝗺𝗶𝗴𝗵𝘁 𝘁𝗵𝗶𝗻𝗸 𝘁𝗵𝗮𝘁 𝘀𝗼𝗶𝗹 𝗶𝘀 𝗷𝘂𝘀𝘁 𝘀𝗼𝗶𝗹. Responsible for kids' dirty knees. Brown stuff that plants grow in. But when constructing on a soil base its mechanical properties have to be very well understood. And the structure of natural soil and "𝘳𝘦𝘮𝘰𝘭𝘥𝘦𝘥" (worked, manipulated if you like) soil can be very different which can drastically alter its mechanical and water holding properties. This paper https://lnkd.in/ePQyDhpi reports the analysis of 𝘱𝘰𝘳𝘦 𝘤𝘩𝘢𝘳𝘢𝘤𝘵𝘦𝘳𝘪𝘴𝘵𝘪𝘤𝘴 of natural structured clay applying the combination of scanning electron microscopy (SEM) and 𝗺𝗲𝗿𝗰𝘂𝗿𝘆 𝗶𝗻𝘁𝗿𝘂𝘀𝗶𝗼𝗻 𝗽𝗼𝗿𝗼𝘀𝗶𝗺𝗲𝘁𝗿𝘆 (MIP). In this particular case remolded clays had large pore openings (approximately 2 to 200 μm) which were effectively absent in the naturally compacted samples. SEM confirmed particle rearrangement. MIP was done using PoreMaster 33 porosimeter https://lnkd.in/d297YB84 . Porous Material Characterization #soil #soilmechanics #porosity Image: NOT from cited paper, just for illustration.
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So with the flow of analysis, I came to know that incubation time period can also provide hindrance in plotting a calibration curve in case of UV Spectroscopy. So we had to repeat the experiment in order to get the desired #regression square value i.e. r-square. What do you think? #selfevaluation #experimentalstudy
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Micrograph time! This wonderful image shows a cross-section of multiple layers of metal in an evaporator, primarily consisting of silver and aluminum. The metal layers were cross-sectioned using a focused ion beam (FIB) instrument, which produced a defect-free surface finish. Tiny grains are evident even at this 10,000x magnification, owing to the high-quality surface finish and expert use of the scanning electron microscope. Many thanks to our SEM guru, Eric Miller, for this image. #MASTest #MaterialsScience #MaterialsTesting Image description: At least 24 different layers of metal shown in a cross-section view at 10,000x magnification. The tiny grains are visible in many of the layers, as well as some twinned grains. The layers were cross-sectioned using a focused ion beam instrument and then imaged using a cold-field emission scanning electron microscope.
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Dealing with haze in your fluorescence images? With Digital Haze Reduction on the Revolve microscope, blur is reduced to reveal finer details, so you can take clear publication-quality images. discover-echo.com/revolve
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recognised by crystal structure the ultimate method throw out 10X and Microscope.
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Thrilled to share my article on "Numerical Simulation Approach for Low-Velocity Impact Response of Bioinspired Layup Design in GFRP Laminates" has been published as a chapter in a book title "Advances in Applied Mechanics". This study investigates the out-of-the-plane impact response of glass/epoxy (GFRP) composite laminates with a bioinspired helicoidal stacking sequence. The article can be accessed here: https://lnkd.in/gVyFUZzu Feel free to read and share your thoughts! #MaterialsScience #ResearchPublication #BioinspiredDesign #FRPComposites #PolymerComposites
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Material parameters for volume scattering. A volume scatterer is characterized by the scattering and absorption coefficient as well as its phase function. We can retrieve these parameters from experiments, by minimizing the RMS deviation between measurement and simulation. In order to demonstrate the idea and its conceptual difficulties, using input data from simulations instead of measurements is permissible. To make things even simpler, we ignore absorption and use the Henyey-Greenstein phase function which has just one parameter, the anisotropy g. RayJack ONE® simulates diffuse transmission, reflection, and un-scattered transmission of a thin slab, comparing it with input data via the RMS deviation. The left picture shows the simulation setup (slab with a scattering medium enclosed by a sphere which serves as a detector) and the right the RMS as function of scattering coefficient mu and anisotropy g. To retrieve the input parameters, one should take their values at the minimum of the RMS. However, there is no distinct minimum, but a rather narrow “valley”, leaving the result ambiguous. This situation is very typical for such experiments, because multiple scattering always erases system information and thus limits the possibilities for parameter reconstruction. Cleverer measurements using more than one sample thickness are needed to improve the situation. Learn more about RayJack ONE® on our website: https://lnkd.in/dJfMUa3 and our YouTube channel: https://lnkd.in/ex4pbUCZ #RayJackONE #illumination #scattering
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