Samba Scientific’s Post

🔬 Dive into the realm of dynamic structural biology with the latest methods primer on smFRET techniques. Explore the potential of smFRET for a range of applications, from genome maintenance and gene expression, to transmembrane transport and signalling. 💡 The comprehensive methods primer covers: - sample preparation including fluorophore selection - fluorescence microscopes for smFRET studies - data collection in vitro and in live cells - data analysis pipelines - limitations Emphasizing the importance of reproducibility and data transparency, the paper also discusses data deposition in citable open respositories. ➡ Read full text here: https://lnkd.in/euKYJKkf Do you want to incorporate smFRET into your own research toolbox? Perform easy and efficient smFRET experiments with Luminosa, the new single photon counting confocal microscope from PicoQuant. ➡ It`s user-friendly software includes context-based workflows for both diffusing and immobilized FRET samples, and even offers new flexibility with variable PSF feature: https://lnkd.in/ePSdNqzQ To keep analysis simple for you, the equations for single-molecule FRET corrections, which are based on a multi-laboratory benchmark study and discussed in the article are incorporated into the software and corrections are performed automatically in real-time. #Luminosa #smFRET #MicroTime #microscopy

The #MotherMachine is a #microfluidic device used in #singlecell studies, particularly in #microbiology, to observe the growth, division, and dynamics of individual #bacterialcells under precisely controlled conditions over extended periods of time. It is a widely used tool for studying the behavior and lifecycle of cells in a highly controlled microenvironment. The classical #MotherMachine consists of an array of narrow, dead-end channels (typically around 1 μm in width, the size of a single bacterium) connected to a wider flow channel. Cells are loaded into these narrow channels, with one cell (the "mother cell") at the closed end. Nutrient-rich media continuously flow through the wider channel, diffusing into the narrow channels to feed the cells. You can read more about the Mother Machine concept and how to use it on the homepage of its inventor, prof. Suckjoon Jun: https://lnkd.in/dpwiv5vX  For the past 12 years, ConScience has excelled in the production of Mother Machine molds for numerous research groups at Universities such as Uppsala University Harvard University University of Oxford University of Cambridge, and UC San Diego and many more. We offer an accuracy of less than 30 nm for channel sizes as well as the possibility of multiple channel sizes with square cross-section on one and the same mold - opening up unexplored possibilities for mother machine #microfluidics #cellbiology. https://lnkd.in/dugkxUj3 Examples of Mother Machine Molds from ConScience can be found literature, including in Science Magazine and Nature Portfolio Jones, D. et al. Kinetics Of dCas9 Target Search In Escherichia Coli. Science 2017, 357, 1420-1424. https://lnkd.in/dikFS8qn Hammar, P. et al. Direct Measurement Of Transcription Factor Dissociation Excludes A Simple Operator Occupancy Model For Gene Regulation. Nature Genetics 2014, 46, 405-408. https://lnkd.in/dDWYKJbr

A review surveys the state of biomedical nanodiamond research, covering the material’s use in genetics and gene editing, drug delivery systems, neural interfacing, biomedical sensors, synthetic biology, and organ and tissue regeneration. In PNAS Nexus: https://ow.ly/VPEi50SaWgY

🔬🧪I am pleased to share that I participated in the pre-conference workshop on "Innovations in Molecular Biology, Gene Editing, and Protein Workflows" at the International Conference on Innovation and Emerging Technologies 2024, organized by the Faculty of Technology, University of Sri Jayewardenepura on 20th November 2024.  The workshop focused on how advancements in molecular biology, gene editing, and protein workflows are shaping the future of science. It was insightful to learn how these technologies are revolutionizing research efficiency and enabling new possibilities in life sciences.  This experience has broadened my understanding of these cutting-edge innovations, aligning perfectly with my academic and professional goals in emerging technologies.        #MolecularBiology #GeneEditing  #Innovation #EmergingTechnologies #BiosystemsTechnology #ICET2024

🚀🌱 Unlock the Future of Plant Research with Spatial Transcriptomics! 🌱 Exciting advancements are happening at the Earlham Institute, UK, where researchers are using spatial transcriptomics to reveal how plant cells are organized in groundbreaking studies. This cutting-edge technology allows us to visualize gene activity and map its location within tissues with unprecedented detail. Why is this a game-changer? 🔬 Visualize Gene Activity: See where genes are active within plant tissues. 🧬 Deep Insights: Understand cell organization and communication like never before. 🌍 Broad Applications:  Explore its potential in developmental biology, diseases research, and now, plant science. Check out the innovative Vizgen MERSCOPE® platform that’s making these discoveries possible and see how this technology is reshaping our understanding of plant biology. 👉 [Learn More] https://lnkd.in/ehyv_ynD #SpatialTranscriptomics #PlantResearch #EarlhamInstitute #VizgenMERSCOPE

"Curiosity research is very important,” said Olle Kampe, member of the Nobel Committee for Physiology or Medicine, in announcing the prize to Victor Ambros and Gary Ruvkun for discovering microRNA (miRNA). "They were looking at two worms that looked a bit funny and decided to understand why. And then they discovered an entirely new mechanism for gene regulation. I think that’s beautiful,” Kampe said. At #Mirxes, we share the same inquisitive spirit. Our R&D team is dedicated to harnessing the diagnostic potential of miRNA, while our Discovery Genomics team offers cutting-edge multi-omics solutions to support scientists and researchers in their quest for new discoveries. Connect with us to get curious together! #preventivehealthcare #precisionmedicine #biotech #researchanddevelopment #earlydetectionsaveslives #healthcare #cancerearlydetection #miRNA #research #multiomics #genomics

A review surveys the state of biomedical nanodiamond research, covering the material’s use in genetics and gene editing, drug delivery systems, neural interfacing, biomedical sensors, synthetic biology, and organ and tissue regeneration. In PNAS Nexus: https://ow.ly/mQpH50S2APX

🌌 SpaTial Enhanced REsolution Omics-sequencing: New Frontiers of Spatial Multi-omics 🌌 Revolutionary Technology: Discover the synergy of Stereo-seq and DNA Nanoball (DNB) technology, illuminating the cellular landscape with unmatched clarity. Global Impact: Learn how these advancements are redefining the Human Protein Atlas, opening new realms of understanding in tissue analysis. 🎤 Speakers: Dr. Jan Mulder: Group leader @ Karolinska Institutet. Dr. Yen-Yu Lin: Senior field application @ BGI Genomics Why Engage? Pioneering Insights: Dive into the latest advancements in gene expression analysis, enhanced by spatial precision, and see how they're reshaping our understanding of complex biological structures. Toolbox Evolution: Gain firsthand knowledge of STOmics' newest bioinformatic tools and automation systems, designed to elevate the efficiency and scope of your research projects 📺 Webinar Link 📺 🔗 https://lnkd.in/ehGxaAce #SpatialMultiOmics #GenomicFrontiers #ScientificInnovation

🎉  𝐂𝐨𝐬𝐌𝐱 𝐒𝐌𝐈 2024 𝐖𝐫𝐚𝐩𝐩𝐞𝐝: 𝐂𝐞𝐥𝐞𝐛𝐫𝐚𝐭𝐢𝐧𝐠 110 𝐁𝐢𝐥𝐥𝐢𝐨𝐧 𝐒𝐩𝐚𝐭𝐢𝐚𝐥 𝐓𝐫𝐚𝐧𝐬𝐜𝐫𝐢𝐩𝐭𝐬!🎉 What a year it’s been! In 2024, you propelled spatial biology to extraordinary new heights with CosMx SMI. 🧬110+ 𝐁𝐢𝐥𝐥𝐢𝐨𝐧 sub-cellular, spatially imaged transcripts Providing the highest single-cell plex with accurate cell segmentation, CosMx SMI is empowering researchers globally to unlock deeper insights and drive groundbreaking discoveries in spatial biology. Your dedication to pushing the boundaries of spatial biology and uncovering complex cellular ecosystems is truly shaping the future of research. 👏 𝐂𝐨𝐧𝐠𝐫𝐚𝐭𝐮𝐥𝐚𝐭𝐢𝐨𝐧𝐬 to all our researchers for your passion, collaboration, and ingenuity. Together, we’ve turned big questions into even bigger answers. Here’s to another year of high-fidelity gene expression with CosMx SMI! 🚀 #2024Wrapped #SpatialTranscriptomics #SpatialBiology #CosMxSMI

🧬 Researchers at Rockefeller University have unveiled a groundbreaking view into how every living cell transcribes DNA into RNA, revealing the earliest moments of gene expression. Using cutting-edge cryo-electron microscopy and rapid robotic systems, the team captured the elusive process of RNA polymerase (RNAP) opening the DNA transcription bubble in real time. This feat, detailed in Nature Structural & Molecular Biology, answers long-standing questions about the initiation of transcription and redefines our understanding of this vital cellular process. Dive into the details of this revolutionary study and discover how it could reshape the future of biological research! #Genetics #Science #Innovation Read more: https://lnkd.in/eqBrpEw8