Sameeya Arshad’s Post

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30 HPLC Tips for Freshers 💯🏅⬇️ 1. Use high-quality HPLC columns for optimal results. 2. Regularly check and maintain your HPLC system. 3. Keep detailed records of your HPLC methods and results. 4. Use appropriate mobile phases for your analytes. 5. Optimize flow rates for better separation. 6. Minimize extraneous noise in your HPLC system. 7. Monitor column temperature for consistent results. 8. Use guard columns to protect your analytical column. 9. Inject samples at the recommended volume and concentration. 10. Filter samples to prevent clogging the column. 11. Calibrate detectors regularly for accuracy. 12. Use proper sample vials and caps to prevent contamination. 13. Check baseline stability before each run. 14. Utilize gradient elution for complex sample analysis. 15. Purge solvent lines to avoid mixing mobile phases. 16. Store solvents properly to maintain quality and consistency. 17. Perform system suitability tests routinely. 18. Troubleshoot issues promptly to avoid downtime. 19. Use appropriate detection wavelengths for different compounds. 20. Implement good laboratory practices to ensure data integrity. 21. Consider pH effects on analyte retention times. 22. Optimize injection volumes for sensitivity and resolution. 23. Validate methods thoroughly before routine use. 24. Utilize internal standards for quantification accuracy. 25. Stay updated on new HPLC technologies and techniques. 26. Collaborate with colleagues for method optimization insights 27 .Keep a clean working environment to prevent contamination 28 .Regularly replace consumables like filters, seals, and tubing 29 .Use software tools for data analysis and method development 30 .Attend conferences or workshops to stay current in the field

  • 30 HPLC Tips for Freshers



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