Excited to share my fifth and final first-author publication from my Ph.D. with Prof. Ramon Gonzalez, recently published in Bioresource Technology! In this study, we explored synthetic metabolic pathways for the use of methylsuccinate —a key precursor from the anaerobic activation of methane via fumarate addition. Through a combinatorial approach of enzyme mining and adaptive laboratory evolution, we engineered multiple E. coli strains growing efficiently on methylsuccinate. In addition, a novel electron transfer pathway involving heterologous flavoproteins and quinone oxidoreductase was elucidated. We hope this engineered strain will serve as a stepping stone toward synthetic methanotrophy, advancing biomanufacturing using methane as a sustainable feedstock. It’s been a rewarding journey, and I’m excited to see where this research can lead! #biomanufacturing #syntheticbiology #methanotrophy #sustainability #biotechnology More details can be found at:
Nico Claassens
1mo
Great work!!
Nils Averesch
1mo
Oh, this is super interesting! I was intrigued by the same metabolic mechanism a few years ago.
Jong-Won Lee
1mo
Congrats Seung Hwan!
Congrats Allen!!!
Biomedical Engineering PhD student• Synthetic Biology • Protein Engineering • Machine Learning • AI
1mo👀 excited to read soon!