Yichun Wang’s Post

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Assistant Professor, University of Notre Dame

Happy to share our latest publication led by our PhD student James Johnston and Hyunsu Jeon at ND Chemical and Biomolecular Engineering in Biomaterials Science – recognized as an Emerging Investigator article! Small extracellular vesicles (sEVs) are emerging as nanocarriers in drug delivery, but scaling their production for clinical applications remains a challenge. In our new work, we tackle this problem with a novel 3D culture platform that significantly boosts sEV production. 💡 Key innovation: We developed a piezoelectric nanofibrous scaffold (PES), coupled with acoustic stimulation, to mimic a natural 3D microenvironment. This platform led to a 15.7-fold increase in sEV production per cell, with particle size and protein composition consistent with standard methods. 🔬 How it works: 3D cell culture + acoustic stimulation synergize to activate key pathways involved in sEV production. Cell morphology shifts in 3D lead to cytoskeleton redistribution and enhanced intracellular vesicle trafficking. Many thanks to our collaborators who contributed to this work, and to Biomaterials Science for recognizing us with the Emerging Investigator honor. Read more: https://lnkd.in/ghXcMr2v #DrugDelivery #Biomaterials #ExtracellularVesicles #3DCellCulture #EmergingInvestigator #ScientificInnovation #Biotech #BiomedicalScience

Stimulative piezoelectric nanofibrous scaffolds for enhanced small extracellular vesicle production in 3D cultures

Stimulative piezoelectric nanofibrous scaffolds for enhanced small extracellular vesicle production in 3D cultures

pubs.rsc.org

Amazing work 🙂 Vesicles tend to have lower encapsulation efficiency compared to Lnps, thus enhancing number of vesicles delivered is a welcome change 👍 insightful direction for the field 😊

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