Dunn Labortechnik GmbH

The GlucCell® glucose meter together with the suitable test strips from Esco Bioengineering have been part of the Dunn Labortechnik programme for many years. The proven, portable system offers unique opportunities to users in the fields of cell cultivation research and process development to follow the course of the glucose concentrations and thus the metabolic processes almost in real time, with high precision and at comparatively low costs. The GlucCell® glucose meter is therefore often used for process monitoring and process optimization.   In contrast to diabetes glucose measuring devices, the GlucCell® glucose meter has been optimized for measurements in serum-free and serum-containing media in the field of animal and insect cell cultures.   The GlucCell® glucose measuring system is easy to use, is versatile and portable and offers a high degree of accuracy and precision of the measured values (90 and 95 %).   As only 1.5 µl sample volume is required for each individual measurement and results are displayed after only 15 seconds, even small volume cultures can be continuously monitored, and intermediate measurements can easily be made.

High Quality Liquid Stable Substrates and Conjugates by MOSS The stable liquid substrates and conjugates from MOSS have been part of Dunn Labortechnik’s portfolio for many years. With over 34 years of experience, MOSS manufactures immunoassay reagents in batch quantities of up to 2000 litres with high lot-to-lot stability. MOSS’ liquid substrates for horseradish peroxidase (HRP) and alkaline phosphatase (AP) have a shelf life of up to 36 months when stored refrigerated (2 - 8 °C) and can be used for ELISA, immunoblot and immunohistochemistry. Various soluble and insoluble substrates such as TMB variants, ABTS, DAB, p-NPP, BCIP/NBT and Fast Red are available. The specialized conjugates from MOSS can be used in various immunoassays, such as microarray, Western blot, flow cytometry and FLISA (fluorescence-linked immunosorbent assay). Usage in multiplex and bead-based methods such as Luminex™ and Applied BioCode® are possible as well. Numerous conjugate combinations, such as streptavidin-phycoerythrin (SAPE), immunoglobulin (Ig)‑phycoerythrin, streptavidin-HRP or streptavidin-AP, or Ig-HRP and Ig-AP are available and can be stored for up to 36 months when cooled. Please contact Dunn Labortechnik directly for detailed information or visit our website www.dunnlab.de.  #ELISA #Immunoassays #flowcytometry #FLISA #Luminex #immunoblot

User-friendly “Guardian®” Class II microbiological safety cabinets from Monmouth Scientific The latest generation of laminar airflow systems manufactured by Monmouth Scientific and distributed by Dunn Labortechnik, are the “Guardian” Class II Microbiological Safety Cabinets. The “Guardian” utilizes a specially ventilated enclosure, developed for sterile material handling and sensitive processes where biological samples are applied requiring a safe work zone, providing protection for the user, samples and surrounding environment, as well as 5 Year Warranty on parts. Manufactured to the highest quality standards, the state-of-the-art biosafety solution is equipped with high-quality HEPA filters (99.997% efficient at 0.3 microns), creates an ISO Class 4 Clean Environment. Further features include an intuitive control system, energy-efficient LED lighting, and a Visionaire® Touchscreen Control Interface for controlled airflow and UV sterilization functions. Class II biological safety cabinets are suitable for work with microbiological agents assigned to Biosafety Levels 1, 2, 3 or 4. Please contact us for detailed information or visit our homepage under www.dunnlab.de.

Dunn Labortechnik presents the BelloCell® Bioreactor system from Esco Bioengineering for high density cultivation in CO2 incubators of adherent growing cells in both serum-containing and serum-free culture medium. Available are different bioreactor system configurations for numerous animal mammalian and insect cells applications ranging from protein expression and production of non-secreted proteins to the production of (stem) cells, secreted proteins, viruses and monoclonal antibodies. Selecting a suitable combination of carriers, bioreactor bottle and cultivation method, large amounts of protein (miligrams to grams), high numbers of virus particles (10^11-10^12 pfu) and high cell densities (e.g. 10^9/gram BIONOC macrocarriers) can be produced eliminating the need to maintain numerous spinner flasks, dozens of roller bottles or hundreds of T-flasks.      The large specific surface area of up to 2730 cm^2 per gram of the BioNOC II Macrocarriers and specific design of the flexible reactor bottles allow precisely controlled, alternating exposure of microcarriers to air and culture medium (tidal flow principle). Thus, a perfect supply of immobilized cells with nutrients and oxygen is guaranteed and at the same time only low shear stress is applied during cell cultivation. With the newly developed, grid-like BioMESH® macrocarriers, Esco Bioengineering now offers an additional carrier system that has been specifically developed for the cultivation of stem cells (MSCs) and fibroblast-like cells and allows particularly efficient and low-particle recovery of cells. Using the BelloFeeder® Pump Module, the system can be easily converted to a continuous cultivation bioreactor using standard glass bottles as external medium reservoir. 

Dunn Labortechnik offers from Chemglass Life Sciences a wide range of borosilicate glass reactor systems including various sizes of Process Filter Reactors. The Chemglass filter reactor systems are a good choice for the realization of chemistry processes which include mixing as well as vacuum or gravity filtration steps. The range of standard products comprises jacketed and unjacketed benchtop filter reactors with 1-5 liters reaction volume as well as larger filter reactor systems (10-20 liters) with suitable castor stands. Furthermore, customized systems with reaction volumes of up to 65 liters and a unique swing-mounted filter base design are available on request. The Chemglass Filter reactor systems have a PTFE filter bottom consisting of a filter base, a perforated filter support plate and a 30 µm porous PTFE filter. Different types of stirrers, detachable drain valves, adapters, and spill trays complete the wide product range. Optionally available for all reactor systems: jacketed vessels for temperature control via external systems (liquid-based cooling/heating systems, circulators). A wide range of accessories such as temperature and pH probes, powder addition funnels, reflux coolers and suitable adapters complement the basic systems and allow easy handling by the user. Additional modules such as distillation shelves, carts on wheels and gas scrubbers enable easy separation of volatile components and environmentally friendly process design. The special design of the Chemglass reactor systems allows easy installation of the components and handling of the system. Due to the compact design, the reactor systems fit under most standard fume hoods.

New in our portfolio: ECIS® from Applied BioPhysics We are happy to announce our new partnership with the US-based company Applied BioPhysics. As new exclusive distributor for the DACH-region, Belgium and the Netherlands, we will be supporting existing customers and take care of new enquiries. Applied BioPhysics, in the market for over 3 decades, is known for Electric Cell-substrate Impedance Sensing (ECIS®) for non-invasive and label-free monitoring of cells. They offer the system Z-Theta which measures impedance and capacities via gold electrodes in cell cultures. These measurements are useful for a wide range of possible applications such as barrier function, cell migration, cell proliferation, cytotoxicity, wound healing, drug research and many more.   In addition, a wide range of accessories such as 8-, 96- or 24-well (transfilter) arrays are available. The arrays are equipped with 1, 10 or inter-digitated finger electrodes and come with plates made from PS. 8-well transfilter arrays to measure TEER (Transepithelial electrical resistance) or flow channel arrays mimicking shear stress in vivo are also available. All arrays can be put under an inverted microscope for observation of cells and come with a barcode. Arrays with small electrodes can better observe individual behaviour of cell proliferation or migration whereas arrays with many electrodes provide the advantage of smoothing out small differences and lead to a more average and statistically meaningful result. A specific tool of ECIS® for wound healing is integrated in the user-friendly software of the device. High currencies for a defined period of time are applied to kill the cells over the electrode. If these voltages are used for a very short period even electroporation methods are possible. Please contact us to learn more about the innovative ECIS® method and the products from Applied BioPhysics.   Contact: Dr. Kevin Denkmann • Managing Director Dunn Labortechnik GmbH • Thelenberg 6 • DE-53567 Asbach Tel. +49 (2683) 4 30 94 • E-Mail: info@dunnlab.de • Internet: www.dunnlab.de

Opportunity for smaller budget: 4-channel Cobra Nano Dispenser demo system from Art Robbins Instruments Dunn Labortechnik presents the 4-channel Cobra Nano Dispenser from Art Robbins Instruments. The “Cobra” is ideal for preparation of PCR Master Mixes, biochemical assays and drug development. The dispenser has a deck with two plate positions, can hold full- and semi-skirted PCR plates and any ANSI/SLAS sized plate in 96-, 384- or even 1536-well format. Aspiration is possible from deep-well blocks, but also from 0.2 ml, 1 or 2 ml tubes. The system does not need any disposables - thus saving overall costs. The wash system is integrated into the “Cobra”. The “Cobra” can dispense volumes down to 300 nl, but can also fill plates with up to 5 ml per well through a bulk dispense modus. The 4-channel system can dispense any volume in any well with up to 4 independent reagents in the same pass. Mixed modes between bulk dispense and aspirate/dispense can also be combined in one protocol. Dispensing gradients are another feature of the system. The Windows based software is easy to use and the system is supplied with multiple licenses and a PC. It can also be integrated into automatic systems for storage of plates. We currently offer one demo system in stock for approx. 30 % off list price while still granting one year warranty. Please contact us for further information or visit our webpage www.dunnlab.de.

Do not forget to visit us at HEC-26 in Krakow next week! We are looking forward to see all the long-time ARI users and also some new faces. #proteincrystallography #liquidhandling #labautomation