[en] The primary circuit pressure boundary check valves of 320 MW pressurized water reactor is a nuclear grade I key equipment. The sealing demand is very high, which is directly related to the internal leakage rate of the primary circuit system. After the welding check valve is repaired, the sealing performance is judged by color printing checks. If there is water or humid vapor in the pipe, it will affect the accuracy of the color printing checks. For the particularity of the online check valve tightness test, online detecting device is designed by the hydraulic pressure drop method in other nuclear power plants, but the method has some shortcomings and restrictions. In this paper, we design a reliable and portable test equipment by the low-pressure gas seal test flow measurement, which make accurate and quantitative judgment of sealing property after the pressure boundary check valves are repaired. (authors)

[en] In nuclear power plant, the sealing performance for welding check valve of lst circuit pressure boundary is very high, which is directly related to the internal leakage rate of main system. A low pressure seal for testing online sealing performance of the check valve methods is set up to solve the technical problems of the online seal performance testing for welding check valve. According to the principle of the test method, the corresponding online sealing performance test device and mockups can be designed and manufactured, and in this way the reliability and validity of the testing device can be tested. During the research, the original method can be adjusted, optimized to meet the anticipation of the project through the simulation analysis. As well as the risks of the testing equipment onsite application can be summarized during mockup test and the one-to-one countermeasures can be set down. Testing and application of low pressure seal for testing online sealing performance of the check valve methods, which increased a new online seal performance testing in repair field of nuclear power equipment. (authors)

[en] Conventional island seawater booster pump set of QNPC 310 MWe unit are very important in the whole circulating cooling system, and the integrate function of seawater booster pump outlet check valve is the foundation of steady operation of the seawater booster pump set. The article mainly introduce that through the analyses to the reason to the problem that the seawater booster pump outlet check valve of QNPC 310 MWe unit appeared in past years by our team, and considering the influence of operation condition and circumstance, the team improve the seawater booster pump outlet check valve from swing check valve to shuttle check valve which operate more appropriately in the system. By the test of continuous practice, we make further modification to the inner structure of shuttle check valve contrapuntally, and therefore we solve the problem in seawater booster pump outlet check valve fundamentally which has troubled the security of system operation in past years, so we realize the aim of technical improvement and ensure that the system operate in safety and stability. (authors)

[en] Excessive alcohol consumption can lead to gastric ulcer and the present work was aimed to examine the protective effect of tetrahydrocoptisine (THC) in the model of ethanol-induced gastric ulcer in mice. Fasted mice treated with ethanol 75% (0.5 ml/100 g) were pre-treated with THC (10 or 20 mg/kg, ip), cimetidine (100 mg/kg, ip) or saline in different experimental sets for a period of 3 days, and animals were euthanized 4 h after ethanol ingestion. Gross and microscopic lesions, immunological and biochemical parameters were taken into consideration. The results showed that ethanol induced gastric damage, improving nitric oxide (NO) level, increased pro-inflammatory cytokine (TNF-α and IL-6) levels and myeloperoxidase (MPO) activity, as well as the expression of nuclear factor-κB (NF-κB) in the ethanol group. Pretreatment of THC at doses of 10 and 20 mg/kg bodyweight significantly attenuated the gastric lesions as compared to the ethanol group. These results suggest that the gastroprotective activity of THC is attributed to reducing NO production and adjusting the pro-inflammatory cytokine, inhibited neutrophil accumulation and NF-κB expression. - Highlights: • THC decreased ethanol-induced pro-inflammatory cytokine release. • THC inhibited the production of NO in serum and gastric tissue. • THC reduced NF-κB expression and MPO accumulation in ethanol-induced gastric tissue

[en] Objective: To assess the personal dosage level of external exposure for radiation workers in YangPu. Methods: Thermoluminescence dosimetry was applied to measure the personal extemal radiation dosage of workers exposed to radiation. Results: Totally 2540 radiation workers were monitored from 2009 to 2012. The average annual effective dosage was 0.75 mSv/a. The workers receiving annual dosage equivalent less than 2 mSv accounted for 99.17%. The average annual individual effective dosage was higher in the nuclear medicine workers than those of other professions. Conclusion: Most of the radiation workers in YangPu District are safe in the working conditions according to the related national standards. The radiation protection of nuclear medicine should be focused in the future. (authors)

[en] Pipe dead end conditions in primary loop pressure boundary of nuclear power plant easily lead to equipment defects that have a significant impact on the coolant leakage rate control, and it is hard to deal with the defects. By analysis of the first domestic project of pipe dead end check valve and defected pipeline replacement in a nuclear power plant, it proved that the project reliability can be improved through conservative analysis and selection of technical solutions, project risks identification and classification by failure tree analysis, risk control measures determination by fishbone diagram analysis and phased process control. (authors)

[en] Objective: To evaluate the multislice CT(MSCT) characteristics of appendiceal mucoceles and mucinous tumors. Methods: MSCT of appendiceal mucoceles (3 patients) was compared with that of mucinous tumors (6 patients). Results: Appendiceal mucoceles were round, oval or tubular cystic lesions with thin walls and no contrast enhancement. The mucinous tumors included mucinous adenomas (5) and mucinous adenocarcinoma (1). The mucinous adenomas appeared as cystic masses with heterogeneously enhancing regular thick walls. Mural calcification was found in 1 of the 5 patients. In one patient with mucinous adenocarcinoma, the tumor wall was irregular and thick with heterogeneous contrast enhancement. Conclusions: MSCT characteristics of appendiceal mucoceles and mucinous tumors are helpful in differentiating benign from malignant lesions. (authors)

[en] We report on a method for selective fluorometric determination of Ag(I) based on the use of graphene oxide (GO) and by exploiting exonuclease III-assisted signal amplification. In the presence of Ag(I), the self-hybridization of cytosine-rich ss-DNA labeled with the fluorescent tag FAM leads to the formation of a ds-DNA that is cleaved by the catalytic action of Exo III. In the absence of Ag(I) ions, however, the tagged ss-DNA is adsorbed on the surface of GO and its fluorescence is quenched. As a result, the induction of the process by Ag(I) leads to a strong amplification of fluorescence. The method allows Ag(I) ions to be determined in the 1 to 250 nM concentration range, with a 0.1 nM detection limit. The recoveries range from 93.3 to 105.1 %, with RSDs of <8.2 %. (author)

[en] Acute liver injury (ALI) is characteristic of abrupt hepatic dysfunction and inflammatory response. Activaion of Kupffer cells (KCs) plays a central role in the pathogenesis of ALI. Since the High Mobility Group A protein2 (HMGA2) occurs as a driver at critical stage of hepatocellular carcinoma, herein, we investigated the role of HMGA2 in macrophage activation during ALI. Our study found that the expression of HMGA2 decreased dramatically both in KCs isolated from the liver in mice with ALI and in LPS-induced RAW264.7 cell lines. Moreover, loss- and gain-of-function studies suggested that HMGA2 could enhance the expression of pro-inflammatory cytokines including TNF-α, IL-6 and IL-1β. These results indicated that HMGA2 may play an essential role in macrophage activation during ALI. Additionally, our results showed the expression of HMGA2 was up-regulated when LPS-induced RAW264.7 cells were treated with 5-aza-2-deoxycytidine. Furthermore, silencing of DNMT1, DNMT3a, DNMT3b could respectively prevent the down-expression of HMGA2 in LPS-induced RAW264.7 cells. In conclusion, HMGA2 promotes the release of pro-inflammatory cytokines through NF-κB pathway, and the dysregulation of HMGA2 may involve with hypermethylation. - Highlights: • HMGA2 was screened through RRBS by our research group. • Our study provides the first evidence to demonstrated the function of HMGA2 in acute liver injury. • HMGA2 mediated inflammation is associated with hypermethylation was illustrated for the first time.