[en] Polymerase chain reaction (PCR) is an important diagnosis tool in molecular biology, which have been greatly improved by PCR. However, optimizing the experimental conditions is still a problem for PRC. Computer biology can be a solution to this problem. In this paper, developments of the mathematical models for PCA are reviewed. It is believed that this kind of research efforts shall be helpful for optimizing the experimental conditions and providing guidance for the biologists and understanding the mechanism of PCR. (authors)

[en] A mathematical model of polymerase chain reaction (PCR) containing uncertain and time-varying parameters has become important for model-based guidance experiment design. In this study, the local and global sensitivity analyses were conducted to identify that the responses of PCR process vary with their parameters of initial reactant concentrations and rate constants. Our results showed that the template concentration in initial reactant concentrations had the largest effect on DNA amplification yield. The rate constant characteristics showed that the local sensitivity basically determined the specific reactions; and the global sensitivity, the non-specific reactions. Our work should be helpful for optimizing PCR experimental conditions, and determining the PCR parameter sensitivities. (authors)

[en] The cross-section ratios of the transfer ionization to the single electron capture of He and Ne induced by oxygen ions were measured by means of position-sensitive detecting and the time-of-flight of scatter ions-recoil ions. It is found that the tendency of measurements is consistent with that of references. In addition, explanation of measurements is given. (author)

[en] The transmission of 18-keV O^- ions through Al₂O₃ nanocapillaries with 50 nm in diameter and 12 μm in length is studied in this work. By measuring angular distribution of transmitted particles when capillaries were tilted with respect to incident ion beam, two peaks were observed. It is distinguished that one of them is composed by direct transmitted ions and another one is composed by scattered ions. A phenomenon referred to as guiding effect, as found for highly charged ions and low-energy electrons, was observed. When negative ions (18-keV O^-) are transmitted through nanocapillaries, most of them were ionized to neutral atoms and even positive ions. The intensity of transmitted particles (O^-, O⁰, and O⁺) decreased when the tilt angle increased. In transmitted particles, the fraction of O^- declined but that of O⁰ and O⁺ ions grew when the tilt angle grew. Both elastic collision and electrostatic scattering were found in scattered ions.

[en] The cross-section ratios of the transfer ionization to the single electron capture of neon induced by oxygen ions were measured by means of position-sensitive detecting and the time-of-flight of scatter ions-recoil ions. It is found that the tendency of measurements is consistent with that of references. In addition, explanation of measurements is given. (author)