[en] The regulation effects of lithium and lymphocytes on hemopoiesis were studied in three groups of experiment. The data obtained from these experiments showed that: (1) There occurred four kinds of reaction of patients lymphocytes to PHA and ConA in vitro; (2) Lithium could induce CFU-S to proliferate and differentiate directly or indirectly; (3) The suppressive effect of lithium upon T_s cells was stronger than upon T_H cells, so it could directly regulate the inhibitory effects of T_s cells upon hemopoietic cells

[en] Kinetics and radiosensitivity of human lymphocytes were studied by the techniques of monolayer agar culture and liquid culture in vitro. In the experiments of lymphocyte kinetics, PHA was designated as a motogen for T lymphocyte. LPS, MEBC and BSA were chosen as mitogens for B lymphocyte. The data from thses experiments showed that under the alone or combination stimulation of LPS, MRBC and BSA, B lymphocytes developed to form colonies in agar culture (0.3%) with the same manner. The stimulation of LPS to B lymphocytes was most significant. By the day 6 after seeding, the numbers of colonies in agar culture were maximal. Whereas the numbers decreased significantly by the day 8. The number of T lymphocyte colonies increased with culture time within 12 days. The peak of ³H-TdR incorporation into T lymphocytes in liquid culture occured at 5th day after seeding. The data above-mentioned demonstrated that the kinetics of lymphocytes cultured in two kinds of environments were different. The studies of the radiosensitivity of T lymphocytes showed that the decreasing in the number of colonies and rate of ³H-TdR incorporation varied in different dose ranges. In the range of 0∼1.0 Gy, r = -0.96, D₀ value was 1.71 Gy for TL-CFC in agar culture, r = -.96, D₀ value was 4.34 Gy for the proliferation T lymphocytes in liquid culture. In the range of 1.0∼6.0 Gy, r were -0.99 and -0.98, the D₀ were 5.88 and 7.36 Gy respectively. The declining tendency in colonies formed by BL-CFC was the same as that of TL-CFC, r = -0.97, for the range of 0∼1.0 Gy, r = -0.97, for the range of 1.0∼3.0, the D₀ values were 1.35 and 4.36 Gy respectively. The results from these experiments shown that the colony technique was a good method for the study in radiosensitivity

[en] The fetal liver is one of the major sites of hematopoiesis during gestation. Under erythropoietin (EPO) stimulation, in erythroid precusor cells of fetal liver, proliferation and differentiation occurred and function of metabolism was enhanced. The technique of ³H-TdR incorporation was used to measure the function of fetal liver cellular DNA synthesis. As EPO concentration at the range of approximately 20 ∼ 100 mU/ml, the counts of ³H-TdR incorporation into fetal liver cells increased. As the concentration of EPO increased, however, its incorporation counts are lower than that in bone marrow of either the fetal or the adult. It suggested that precusors of erythrocyte of fetal liver has differentiated to later phases with the progressive accumulation of mature cells, therefore, both proliferation and function of metabolism are more or less decreased respectively. Under EPO stimulation, however, precusor of erythroid of fetal liver can greatly increase potential effects on DNA synthesis

[en] The effect of ⁶⁰Co γ-rays on lymphocytes induced by Con A and LPS and the relationship between these two groups of cells were investigated by means of ³H-TdR incorporation. The study showed that in vitro, Con A cells were able to promote the inducing effect of LPS to B cells. When Con A cells were irradiated by 10 Gy γ-rays, the ³H-TdR incorporation value reduced significantly and the stimulating effect of Con A cells on LPS cells disappeared. Having been irradiated by γ-rays, LPS cells were not be able to be stimulated by normal Con A cells. When the groups of cells were incubated together after irradiation, the synergistic function disappeared, furthermore the suppressive effect of Con A cells on LPS cells emerged. When these two groups of cells were investigated by means of agar culture, the suppressive effect of 10 Gy γ-rays on lymphocytes colony formation was more obvious. Tests on 7 patients who were suffering from carcinoma of nasoparynx showed that after a course of treatment with ⁶⁰Co γ-rays, the incorporation value in Con A cells became much smaller, the stimulating effect of Con A cells on LPS cells disappeared. LPS cells could not be stimulated by normal Con A cells. The study demonstrated that the radiosensitivity of Con A cells is higher than that of LPS cells

[en] The effect to ⁶⁰Co γ-rays on lymphocytes induced by Con A and LPS and the relationship between these two groups of cells were investigated by means of ³H-TdR incorporation. The study showed that in vitro, Con A cells were able to promote the inducing effect of LPS to B cells. When Con A cells were irradiated by 10 Gy γ-rays, the ³H-TdR incroporation value reduced significanly and the stimulating effect of Con A cells on LPS cells disappeared. Having been irradiated by γ-rays, LPS cells were not able to be stimulated by normal Con A cells. When the groups of cells were incubated together after irradiation, the synergistic function disappeared, furthermore the suppressive effect of Con A cells on LPS cells emerged. When these two groups of cells were investigated by means of agar culture, the suppressive effect of 10 Gy-γ-rays on lymphocytes colony formation was more obvious. Tests on 8 patients who were suffering from carcinoma of nasopharynx showed that after a course of teatment with ⁶⁰Co γ-rays, the incorporation value in Con A cells became much smaller, the stimulating effect of Con A cells on LPS cells disappeared. LPS cells could not be stimulated by normal Con A cells. The study demonstrated that the radiosensitivity of Con A cells was higher than that of LPS cells