[en] Purpose of the present study is to report and disseminate an initial experience gained with the ⁹⁹mTechnitium sodium pertechnetate eluate; obtained after second elution of the ⁹⁹Moly-^99mTc column generator within 3-4 h on the same day; influences the labeling efficiency of ^99mTc labeled RBC while performing by in vitro method in the hospital pharmacy laboratory. Eluate from ⁹⁹Moly- ^99mTc column generator was used consisting radioactive concentration of 50 μCi in 1 ml of normal young healthy human volunteers (n = 27, male =14 and female =13) has not consumed any interfering synthetic chemicals/drugs blood collected in the heparinised syringe after taking informed consent duly approved by Institutional Ethics committee. SnCl₂.2H₂O used as reducing agent for ^99mTc of 2 μg/ml of concentration with mixing, incubating 20-30 min, separating packed red cells, washing with 0.9% saline and then counted in the calibrated Gamma ray Spectrophotometer. Percentage labeling efficiency of ^99mTc-RBC was calculated. Statistical variation Mean ± SD was calculated. Labeling efficiency by in vitro method of RBC collected from human volunteers blood with the ^99mTc was showing poor percentage of 56.5 ± 3.8 as compared to the expected standard percentage binding of ≥90%. Result analysis reflecting that ^99mTc0₄ − eluate used for RBC labeling may be containing more ground Technetium (⁹⁹Tc (ground)) that means ratio of ^99mTc to ⁹⁹Tc (g) may be very low, which has interfered with the ^99mTc (Gamma emitter 140 keV). Limitation of the study is that ⁹⁹Tc (g) in the eluate from the column generator has to be quantified with the suitable and feasible method at different intervals to provide more evidence. Study concludes that ^99mTco4 − eluted for the red blood cell labeling should be fresh and should not be used if the generator has been eluted earlier within 3-4 h. (author)

[en] A unique UV-Curing method is provided for decontamination of radioisotopes on the surfaces which has radiation dealing facilities. In this method acrylic UV-Curing resin was poured on a contaminated simulated surface followed by UV irradiation. The resin interacted with the contaminated surface and after polymerization, the solidified resin was then stripped off, leaving behind a clean surface. The present study is the proof-of-concept of a novel application of acrylic resin UV-Curing methodology for Radioactive surface contamination removal to create a economical clean, effective and safe environment with minimal waste generation post processing ready for environment friendly incineration. (author)

[en] To assess the association of inflammatory markers with known risk factors and short-term outcome of well-differentiated thyroid cancer. Well-differentiated nonmetastatic thyroid cancer patients diagnosed and treated between September 2015 and December 2019 at Kasturba Hospital, Manipal, India, were retrieved for the study. Patient's presurgical blood parameters were noted, and neurtrophil-to-lymphocyte ratio (NLR) and lymphocyte-to-monocyte ratio (LMR) were calculated. Clinicopathological details along with tumor markers at baseline and at 6 month's follow-up were tabulated. Patients were categorized as complete disease clearance if their clinical examination was normal, stimulated thyroglobulin (Tg) was <1 ng/ml, Anti-thyroglobulin antibodies <65 IU/L or showing a decreasing trend, and follow-up I-131 whole-body scan was negative. The association of the inflammatory markers with known risk factors and short-term outcomes were compared. A total of 272 patients were analyzed in the study. The median NLR in our study cohort was 2.55 (mean = 3.96 with standard deviation (SD) =4.20) and the median LMR was 3.72 (mean = 3.79 with SD = 1.94). The disease clearance rate of our study cohort was 73.9%. The median NLR (2.4 vs. 3.1) and LMR (3.13 vs. 3.93) were significantly different among the patients with complete disease clearance and those with persistent disease (P = 0.008 and P = 0.003, respectively). The known risk factors such as multifocality (P = 0.04), tumor size (P = 0.013), lymph node metastases (P = 0.001), and baseline Tg (P ≤ 0.001) were significantly associated with persistent disease at 6 months. The NLR showed a positive correlation and LMR had a negative correlation with the known risk factors, however, the associations were not statistically significant. The NLR and LMR are simple yet potential prognostic tools in well-differentiated thyroid cancer. (author)

[en] The purpose of this study was to assess the association of measured glomerular filtration rate (mGFR) using camera-based method with early transplant outcomes. Diethylenetriamine pentaacetate renograms of all voluntary kidney donors between January 2016 and December 2022 at Kasturba Hospital, Manipal, India, were retrieved for the study. Recipient's post transplant biochemical parameters were collected and compared against donors with scaled mGFR >80 ml/min/1.73 m2 (Group 1) and with mGFR between 60 and 80 ml/min/1.73 m2 (Group 2). Donor-recipient pair age, anthropometric parameters, and their differences were also assessed against the immediate transplant outcome. Post transplant immediate graft function was assessed by post transplant nadir serum creatinine, day to achieve nadir serum creatinine, the incidence of slow graft or delayed graft function, and serum creatinine at 1-month post transplantation. Recipients with serum creatinine of >2.5 mg/dl on post transplant day 7 were taken as slow graft function. A total of 161 donor-recipient pairs were analyzed in the study. In recipients who showed persistently high serum creatinine post transplant, older donor age(p < 0.001), higher difference in body mass index among the donor-recipient pair (p= 0.03), and mGFR <80ml/min (p < 0.001) were significantly associated. Slow graft function was significantly more in Group II recipients, with donors having mGFR <80ml/min as compared to Group I with mGFR >80 ml/ min (37.3% vs. 10.6%) (P < 0.001). Camera-based mGFR using Gate's formula is a reliable tool to predict inferior graft outcomes in the immediate post transplant period. Kidneys from donors with mGFR of 60-80 mL/min/1.73 m2 are likely to experience slow graft function in the immediate post transplant period. (author)