[en] Objective: To explore the incidence and significance of intra- and extraosseous edema accompanying benign tumors and tumor like diseases. Methods: Imaging studies including MR, CT and plain radiograph were retrospectively reviewed in 245 cases of benign osseous tumors and tumor like diseases proved by pathology(borderline tumor, cases associated with pathological fractures, cases with lesions of skull were excluded from the study). The incidence of bone marrow and soft tissue edema were defined on T₂WI with fat suppression on MRI in all the cases. χ² test was performed for the incidence rate of edema in the benign tumors and tumor like diseases. The pre-operative diagnoses were reviewed, and the influence of edema to the differential diagnosis between the benign and malignant was analyzed. Results: The total incidence of bone marrow and soft tissue edema associated with benign tumors and tumor like diseases was 37.1%(91/245), which included Langerhans cell histiocytosis 85.2%(23/27), osteoblastoma 85.0%(17/20), osteoid osteoma 93.6%(29/31), chondroblastoma 87.0%(20/23). There was no statistically significant difference of edema incidence among the above-mentioned four diseases. (χ² = 6.35, P > 0.05). Both cases of endosteal capillary hemangioma had edema around. No edema was found around the lesions of other kinds of diseases. 41 of 91 cases (45.1%) with edema were misdiagnosed as malignant tumor by MRI pre-operatively. Conclusion: The marrow and soft tissue edema is a common sign in the benign bone tumor and tumor-like diseases, and it is frequently seen in Langerhans cell histiocytosis, osteoblastoma, osteoid osteoma, chondroblastoma. (authors)

[en] Objective: To explore the influence of PUMA on radiosensitivity of pancreatic cancer AsPC-1 cells after Slug gene inhibition by transfected short interferencing RNA (siRNA). Methods: The AsPC-1 cells were infected with MOI 10, 50, 100 for 72 h, respectively. The expression of Slug and PUMA was analyzed by Western blotting and immunohistochemistry methods. The transfected and control cells were exposed to 4 Gy γ-rays. The cells inhibition rate was examined by MTT, Hoechst 33342 and IP double staining. DNA ladder and Giemsa staning was used to observe apoptosis. Results: The relative value of Slug expression was 0.831±0.14, 0.546±0.12 and 0.178±0.08 after AsPC-1 was infected with Slug-siRNA (MOI 10, 50, 100) for 72 h, significantly lower than that of control group (F=4.992, P<0.05). The relative value of PUMA was 0.325±0.07, 0.593±0.11 and 0.978±0.12, after AsPC-1 was infected with Slug-siRNA (MOI 10, 50, 100) for 72 h, significantly higher than that of control group (F=4.324, P<0.05). The cell proliferation rate was (78.76±9.36)% in transfection combined with radiosensitivity group, significantly higher than that of transfection group [(43.68±6.71)%] and radiosensitivity group alone [(19.25±3.72)%] (F=5.056, P<0.05). The apoptosis of transfection combined with radiosensitivity group was significantly higher than that of others. Conclusions: Slug gene targeting siRNA could inhibit the expression of Slug, and consequently increase the activation of PUMA expression, and so enhance the radiosensitivity to γ-rays. (authors)