[en] Highlights: • Mass spectrometry fragmentation behaviors of steroid hormones were studied and interpreted. • Diagnostic product ions were summarized for characterization of steroid hormones. • A total of 80 and 107 steroidal hormones were characterized or tentatively identified in male and female, respectively. -- Abstract: Steroid hormones, structural derivatives of cyclopentanoperhydrophenanthrene, play important roles in modulation of many physiological processes. Comprehensive characterization of steroid hormones is valuable for understanding the process of human life activities and even disease diagnosis. Hitherto systematical characterization of steroid hormones has been rarely investigated. Here, we presented an integrated method for human urine analysis based on ultra-high performance liquid chromatography-high resolution mass spectrometry in data-dependent acquisition mode with the following parallel reaction monitoring mode. To process the data acquired by two scan modes, a comparative study of standards’ fragmentation behaviors and diagnostic product ions (DPIs) were firstly conducted to facilitate the characterization of steroid hormones. The fragmentation behaviors, DPIs, elemental composition and double-bond equivalent were then simultaneously utilized for systematical characterization of steroid hormones in human urine. Consequently, fragmentation pathways and DPIs for all types of steroid hormones were comprehensively interpreted. It is interesting to find that dehydration is not restricted in the form of hydroxyl groups loss, elimination of the carbonyl oxygen could also generate dehydrated ions. Ultimately, a total of 80 and 107 steroidal hormones were characterized or tentatively identified in human urine of male and female, respectively. The proposed method is expected to provide valuable insights for chemical characterization in complex matrixes.

[en] We describe a compact diffuse optical tomography system specifically designed for breast imaging. The system consists of 64 silicon photodiode detectors, 64 excitation points, and 10 diode lasers in the near-infrared region, allowing multispectral, three-dimensional optical imaging of breast tissue. We also detail the system performance and optimization through a calibration procedure. The system is evaluated using tissue-like phantom experiments and an in vivo clinic experiment. Quantitative two-dimensional (2D) and three-dimensional (3D) images of absorption and reduced scattering coefficients are obtained from these experiments. The ten-wavelength spectra of the extracted reduced scattering coefficient enable quantitative morphological images to be reconstructed with this system. From the in vivo clinic experiment, functional images including deoxyhemoglobin, oxyhemoglobin, and water concentration are recovered and tumors are detected with correct size and position compared with the mammography

[en] This paper aims to discuss the interoperability of compensation topologies for the inductive power transfer system. Based on the common practice of the compensation design, three possible cases which may happen are analyzed, including LCC-S, S-LCC, and LCC-P compensation topologies. In order to evaluate the performance of these three topologies, an analysis method is proposed to investigate the electrical characteristics. The output voltage, current and power of the different topologies affected by variations of the coupling coefficient and the ratio of secondary inductance to primary inductance are analyzed to evaluate their performances. Further simulation models are built to compare the output performance of different compensation topologies. (paper)

[en] Highlights: • Cord serum is a reliable non-invasive tool for monitoring prenatal exposure. • β-HCH and p,p′-DDE were the dominant OCPs in cord serum samples. • There was a positive relationship between the exposure levels of two DDT isomers. Organochlorine pesticides (OCPs) had been widely used in China before they were banned decades ago due to the concern of the toxicology to human. Previous reports showed that OCPs were still often detectable in human bodies. However, there is limited study concerning the body burden of OCPs in infants and fetus in China. In this study, the prenatal exposure to OCPs was evaluated by measuring OCPs in cord serum. A total of 1046 cord serum samples were collected in Wuhan during 2014 and 2015, and analyzed for the concentrations of hexachlorocyclohexanes (HCHs), dichlorodiphenyltrichloroethanes (DDTs), dichlorodiphenyldichloroethanes (DDDs) and dichlorodiphenyldichloroethylenes (DDEs) by using gas chromatography-tandem mass spectrometry (GC–MS/MS). The median levels of ∑HCHs and ∑DDTs were 10.1 ng/g lipid (with the range from p,p'-DDE were found to be the major OCPs presenting in the cord serum samples. A minor positive association between the serum levels of α-HCH and β-HCH was observed, and the concentrations of o,p′- and p,p′-DDT isomers were found positively associated. The OCP exposure levels obtained in this study were comparable to those data from other areas in China and much lower than some of highly polluted countries. The prenatal exposure to OCPs would be of concern since fetuses were more vulnerable than adults and the cord serum is an accurate non-invasive matrix for monitoring of prenatal exposure.

[en] Highlights: • Phthalate metabolite concentrations demonstrated U-trend over three trimesters. • The efficiency in metabolizing di-2-ethylhexyl phthalate decreased during pregnancy. • More recent exposure occurred on early and late gestational periods. • Mothers were more susceptive to DEHP in the early stages of pregnancy. • Multiple samples were needed to evaluate phthalate exposure throughout pregnancy. -- Abstract: Maternal exposure to phthalates may cause some adverse health effects on both mother and fetus, but variations of phthalate exposure and metabolism during pregnancy have not been thoroughly characterized. A total of 946 participants were selected from a cohort study conducted in Wuhan between 2014 and 2015 through which they had provided a complete set of urine samples at three trimesters. Eight phthalate metabolites were analyzed in 2838 urine samples. Based on urinary concentrations, various parameters (i.e. phthalate metabolite concentrations, ratios of metabolites of bis(2-ethylhexyl) phthalate (DEHP) in DEHP, and percentages of individual metabolites in total phthalates) were compared over three visits. We observed that levels of phthalate metabolites showed a U-shaped trend across three trimesters. The significant variations in the ratios of DEHP metabolites indicated that the efficiency in metabolizing DEHP declined during pregnancy and less recent exposure occurred in mid-pregnancy. The changes of percentages of individual compound in total phthalates suggested the inconsistent pattern over trimesters. This longitudinal study found that the exposure pattern, exposure timing and metabolic susceptibility varied by trimesters, which suggests that urine samples should be collected at multiple time points and mothers should be especially careful in the early pregnancy.