[en] Objective: To explore the relationship between irradiation dose and expressions of bcl-2, bax genes in apoptosis process of pancreatic carcinoma cells. Methods: Apoptosis was detected with PI and Annexin V/PI methods and bcl-2, bax proteins in Panc-1 cells after gamma-irradiation were determined with flow cytometry. Results: The results showed that the apoptotic rate increased along with the increase of dose and time within a certain range (≤15 Gy, ≤24 h). The expression of bcl-2 was significantly lower in the irradiated cells than that in the control cells (P<0.05), but that of bax was higher in the former than that in the latter (P<0.05). Conclusion: gamma-radiotherapy can induce apoptosis in pancreatic carcinoma cells through down-regulation of the expression of bcl-2 and up-regulation of the the expression of bax. (authors)

[en] N⁺ ion implantation was used to obtain higher-yield antimicrobial substance. Bacillus subtilis fmbJ was mutated by 25 keV N⁺ ion implantation with the dose of 50 x 2.6 x 10¹³, 80 x 2.6 x 10¹³, 100 x 2.6 x 10¹³, 120 x 2.6 x 10¹³ and 150 x 2.6 x 10¹³ N⁺/m². Results showed that the optimal N⁺ ion dose was 50 x 2.6 x 10¹³ N⁺/m², and a strain of high-yield antimicrobials was obtained and named as Bacillus subtilis fmbJ224. Its antimicrobial substance yield was increased by 96% than the initial. The fermentation characteristic of the strain was studied, and the mode of producing antimicrobial substance for the selected strain was arrearage synthesis type. (authors)

[en] This paper investigates the effects of a new radiosensitizer, doranidazole, and enhancing irradiation on colorectal cancer cells. The radiosensitizing effect of doranidazole was determined using colony formation and propidium iodide (PI) assays to measure cell growth inhibition and the cell killing effect of human colorectal cancer cell lines exposed to high doses of γ-ray irradiation under hypoxic conditions in vitro. Fluorescence staining and cell migration assays were also used to assess the radiosensitizing effect. Cell proliferation evaluated by clonogenic survival curves was significantly inhibited by 5 mmol/L doranidazole, particularly at doses ranging from 10 to 30 Gy of irradiation. The radiosensitizing effect of doranidazole on colorectal cancer cells occurs in a time- and dose-dependent manner. Doranidazole also inhibited the mobility of cell invasion and migration. Doranidazole can enhance the killing effect and the cell growth inhibition of colorectal cancer after high-dose irradiation in a time and dose-dependent manner

[en] An ultrafine-grained (UFG) pure zirconium(Zr) refined by compounding with a size of Φ4 × 6 mm was subjected to a unidirectional compression test using a Gellble-3800 thermal simulation tester at the temperature of 300 °C–450 °C and a strain rate range of 0.001–0.05 s⁻¹. Experimental results showed that the flow stress of UFG pure Zr refined by compounding is highly sensitive to temperature and strain rate, and the peak stress decreases with increase in deformation temperature and increases with increase in strain rate. The Arrhenius constitutive equation based on the experimental data can effectively predict peak stress in actual thermal deformation, The correlation coefficient between the actual value and the predicted value can reach as high as 0.99722. With the increase in deformation temperature and the decrease in strain rate, the UFG pure Zr refined by compounding otably undergoes dynamic recovery and dynamic recrystallization. These findings are based on the hot processing map and microstructure characteristics of UFG pure Zr refined by compounding. The optimal hot-working windows are determined to be in the deformation temperature of 320 °C–360 °C with strain rate of 0.005–0.01 s⁻¹ and the deformation temperature of 410–450 °C with strain rate of 0.003–0.01 s⁻¹. (paper)

[en] In this paper we report ammonia oxidation to nitrogen gas using microbes as biocatalyst on the anode, with polarized electrode (+600 mV vs. Ag/AgCl) as electron acceptor. In batch experiments, the maximal rate of ammonia-N oxidation by the mixed culture was ∼ 60 mg L"−"1 d"−"1, and nitrogen gas was the main products in anode compartment. Cyclic voltammetry for testing the electroactivity of the anodic biofilms revealed that an oxidation peak appeared at +600 mV (vs. Ag/AgCl), whereas the electrode without biofilms didn’t appear oxidation peak, indicating that the bioanode had good electroactivities for ammonia oxidation. Microbial community analysis of 16S rRNA genes based on high throughput sequencing indicated that the combination of the dominant genera of Nitrosomonas, Comamonas and Paracocus could be important for the electron transfer from ammonia oxidation to anode