Nitrogen fertilizer and inoculant effects on N2-fixation by soybean mutant lines as measured by 15N tracer technique
AbstractAbstract
[en] In order to determine the N2-fixing capabilities of several soybean mutant lines under different N rates and inoculant types, two different field experiments were conducted at Antalya and Adana in 1989. In experiment I mutant soybean lines Ck, C2, Cg, Ak, A8, A9 were investigated at 15 and 30 kg N/ha rates of ammonium sulphate (21 %N), where all seeds were inoculated with ''Azotek'' inoculant. In a second experiment, experiment II same mutant lines were investigated using two inoculant - (Entite and Azotek) where 15 kg Na/ha ammonium sulphate (21 %N) was applied. As reference crop, corn was used in all experiments. 15N isotope plot with dimensions of 1.0mx1.5m=1.5m2 were established in each plot. 2.5% 15N atom excess for low N rates and 2.0% 15N atom excess for low N rates and 2.0% 15N atom excess for high N rates were sprayed to the 15N isotope plots as 250 ml aqueous solution just after sowing at both locations. At maturity all plants were harvested at the same time. Total N analysis in ground plant samples were done by micro-Kjeldahl procedure and 15N determinations were made by emission spectrometer. Results showed that increasing the N rate increased the %N dff, %Ndfa and total N fixed by the soybean mutant lines. Also %Ndfa and total N2 fixed varied between the soybean mutant lines according to the inoculant type, the mutant line C-9 had the highest %Ndfa and total fixed N values, among others
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CONTRACT NO. 5307/RB; Research carried out with the support of the IAEA.
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