AbstractAbstract
[en] Exposure to aryl hydrocarbon receptor (AhR) agonists in fish causes lethal disturbances in fish development, but the effects of acute AhR agonist exposure on the cardiovascular system and deformities remain unclear. This study addressed this issue by performing a series of experiments on zebrafish (Danio rerio). The authors hypothesized that genes needed for cardiovascular regulation (PTGS) would exhibit a stronger link to deformities than detoxification enzymes (CYPs). Zebrafish eggs were exposed aqueously until 4 days post-fertilization (dpf) to the AhR agonists benzo(a)pyrene (BaP) or 2,3,7,8-tetrachlorodibenzop-dioxin (TCDD) alone and in combination with the putative AhR antagonists resveratrol or alpha-naphthoflavone (ANF). Gene expression was measured using real-time, reverse transcriptase PCR in zebrafish at 5 and 10 dpf. Although the mortalities did not differ considerably among groups at 10 dpf, the deformities increased significantly after BaP-ANF at 5 dpf and after BaP at 10 dpf, but not after TCDD treatment. CYP and PTGS isozymes exhibited small, but statistically significant changes at 5 dpf. By 10 dpf, the expression returned to control values. In general, CYP1A and PTGS-1 expression at 5 dpf were positively correlated with deformities, while all other genes were negatively correlated with deformities. It was concluded that changes in CYP1A, CYP1C2, and PTGS-1 gene expression at 5 dpf are associated with developmental deformities, but additional work is needed to determine which has the most important mechanistic link.
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Liber, K.; Janz, D.M. (Saskatchewan Univ., Saskatoon, SK (Canada). Toxicology Centre); Burridge, L.E. (Fisheries and Oceans Canada, St. Andrews, NB (Canada)) (eds.); Fisheries and Oceans Canada, St. Andrews, NB (Canada). Biological Station; 188 p; 2009; p. 79-80; The 35. annual aquatic toxicity workshop; Saskatoon, SK (Canada); 5-8 Oct 2008; ISSN 0706-6457; ; Available from Aquatic Toxicity Workshop website at http://www.atw.ca/site_pages/Past Proceedings/Proceedings2008.pdf
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Conference
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