[en] We studied some of the factors influencing seed germination and shoot growth of caper (Capparis spinosa L) and callus formation and regeneration from different parts of the plant (leaf, shoots, fruits) in order to establish a protocol for propagating this plant on a commercial scale. For the dormant seeds, we studied scratching the seed coat using iron particle filings prior to culture on nutrient medium or peatmos. Scratched and non-scratched seeds were also treated with concentrated sulfuric acid for various periods (20, 30, 45, 60 min). Seeds were additionally treated with ultrasonic waves for different periods (15, 30, 45, 60 min). The seeds were also irradiated with 50, 100, and 150 gray of gamma rays to study the effects of irradiation on the germination of caper seeds. Irradiation of the seed with 100 gray dose led to 50% germination one month after culturing in vitro, whereas, no seeds germinated in the control. Irradiation with 150 gray dose led to 18% germination of the seed. As for irradiated seed cultured in the peatmos, 70% of the seed germinated at the 100 gray dose whereas, only 5% of the seed germinated in the control. Treatment of the seed with H₂SO₄ for 20 mints with scratching was very effective in stimulating germination, where the percentage of germinated seed reached 46% compared with 0% in the control. Treatment with just H₂SO₄ for 20 mints resulted in 32% seed germination and treatment with just scratching resulted in only 20% germination. On the other hand, treating the seed with ultrasonic waves did not result in any seed germination. The effect of irradiation was also studied on the growth of caper shoots in vitro. The shoots were irradiated with 10, 15, and 20 gray and subsequently cultured on the MS medium without any growth regulators. The 10 gray dose stimulated shoot growth where average leaf area increased from 1.1 to 2.4 cm². The same irradiation dose, also, led to an increase in shoot length from 1.5 to 2.2 cm. This was accompanied with an increase in shoot rooting percentage from 75 to 100% and with an increase in number of roots per plant. The effects of MS and Rugini media on the growth of caper cuttings collected from the field were studied. The results indicated that the MS medium was better than the Rugini medium with an average length of shoots being, 3.2 cm as compared to 2.4 cm when Rugini medium was used. The influence of different growth regulators on adventitious shoot formation was also studied. It appeared that the use of 2 mg/L ZR and 1 mg/L NAA resulted in 5.5 shoots as compared to 2.2 shoots when only GA3 (2 mg/L) was added to the culture medium. The influence of some plant growth regulators on the formation of callus was also studied using cross section of unripe and variable size fruits. The best media for callus induction were MS9 (BA 1 mg/L, NAA 0.1 mg/L) and MS10 (Kin 0.5 mg/L, 2,4, D 2 mg/L) with more callus being produced on peeled fruits than on unpeeled ones. We studied also the influence of plant growth regulators on the formation of callus studied using leaves and stem parts. The best 2 media for this purpose were MS9 (BA 1 mg/L, NAA 0.1 mg/L) and MS12 (2,4,D 2 mg/L , NAA 1 mg/L, GA3 0.1 mg/L) and the best medium for regeneration was MS6 (KIN 1 mg/L, IAA 0.1 mg/L) which gave an average of 2 plants from every callus piece. (author)