META

Evaluation of the effect of temperature, concentration and volumetric flow in the hydrolysis of sucrose by an immobilized invertase in a spherical reactor

Zamora Leiton, Maria Monserrath (Empresa Bridgstone Costa Rica (Costa Rica)); Molina Cordoba, Manuel (Univ. de Costa Rica, Escuela de Ingenieria Quimica (Costa Rica)); Chacon Valle, Gerardo (Univ. de Costa Rica, Escuela de Ingenieria Quimica (Costa Rica))2011

AbstractAbstract

[en] The effect of the volumetric flow, the temperature and the initial concentration of sucrose in the reaction of hydrolysis of sucrose by immobilized invertase were evaluated in the laboratory. Invertase was immobilized in 20 g of support of mesh size between 120 and 140. The maximum quantity of immobilized invertase obtained has been 0,130 mg/g of support at 220 min. The first experimental stage has consisted in the evaluation of the effect of the initial concentration of sucrose (1,0 and 1,5 mol/L), the volumetric flow (3,0 mL/min and 4,0 mL/min) and the temperature (45 degrees C and 50 degrees C). The effect of the above three variable has been statistically significant. The conversion has been favorable for a concentration of sucrose 1,0 mol/L, a volumetric flow of 3 mL/min and a temperature of 50 degrees C. The maximum conversion obtained has been 95,4 %. The second experimental stage has analyzed the effect of the initial concentration of sucrose (0,75 and 1,0 mol/L), the volumetric flow (2,5 mL/min and 3,0 mL/min) and the temperature (50 degrees C and 55 degrees C). The variable of volumetric flow and the interaction concentration of sucrose - temperature are found statistically significant. The conversion has been favorable for a volumetric flow of 2,5 mL/min, and it has been preferable to work at a temperature of 50 degrees C with an initial concentration of sucrose of 1,0 mol/L. The maximum conversion has been 94,8 %. The effect of the concentration was analyzed in the last experimental stage, it was found that the maximum conversion percentage was 95,0 % for a concentration of 1,1 mol/L, for a temperature of 50 degrees C and for a volumetric flow of 2,5 mL/min. (author)

Original Title

Evaluacion del efecto de la temperatura, concentracion y flujo volumetrico en la hidrolisis de sacarosa mediante una invertasa inmovilizada en un reactor esferico

Primary Subject

INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY (S37)

Secondary Subject

APPLIED LIFE SCIENCES (S60), INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY (S46)

Source

Also available from Biblioteca Luis Demetrio Tinoco, Universidad de Costa Rica; Figs., tabs., refs.

Record Type

Journal Article

Literature Type

Numerical Data

Journal

Ingenieria; ISSN 1409-2441;

; v. 21(no.1); p. 61-74

Country of publication

Costa Rica

Descriptors (DEI)

EVALUATION, EXPERIMENTAL DATA, HYDROLYSIS, IMMOBILIZED CELLS, IMMOBILIZED ENZYMES, SACCHAROSE, SPECTROMETERS, SPECTROPHOTOMETERS, SPECTROPHOTOMETRY, TEMPERATURE CONTROL, VOLUMETRIC ANALYSIS

Descriptors (DEC)

CARBOHYDRATES, CHEMICAL ANALYSIS, CHEMICAL REACTIONS, CONTROL, DATA, DECOMPOSITION, DISACCHARIDES, INFORMATION, LYSIS, MEASURING INSTRUMENTS, NUMERICAL DATA, OLIGOSACCHARIDES, ORGANIC COMPOUNDS, QUANTITATIVE CHEMICAL ANALYSIS, SACCHARIDES, SOLVOLYSIS

LanguageLanguage

Spanish

Reference NumberReference Number

45032388

INIS VolumeINIS Volume

INIS IssueINIS Issue

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