Refeyn

Arvind Pillai’s talk on protein design is almost here!🔬 A researcher in the lab of 2024 Nobel Laureate David Baker, at the Institute for Protein Design, University of Washington, Arvind will present his work on the design of proteins de novo using computational tools💻🧬. It is a step towards the creation of molecular machines that can perform useful functions – like drug release – in response to molecular signals, and uses #MassPhotometry to characterize the resulting assemblies 💊 Register here to hear the full story! https://lnkd.in/dtHhCn-W

When it comes to characterizing protein oligomerization, #MassPhotometry really excels🔬, and his has been shown again in a recent paper – this one explores the structure and biochemistry of a fungal Group 5 septin.  Septins play important roles in membrane organization, polarization and morphogenesis🧬. Group 1-4 septins are well studied, as they are present in humans and yeast, but other groups are not.  Grace Hamilton, Katherine Wadkovsky and Amy Gladfelter – from the University of North Carolina at Chapel Hill and Duke University – characterized the KpAspE Group 5 septin from Knufia petricola, finding that it recapitulates many of the properties of more well-known Septins.  Using mass photometry, they showed that KpAspE formed mainly monomers and dimers at nanomolar concentrations, but its oligomerization behavior was pH-dependent, with trimers appearing at pH 6.4 and dimers becoming more dominant at pH 8.4.  Read the full paper here! 👉 https://lnkd.in/dWzGGE39 #MassPhotometryStories 

Would you like to catch AAV production issues before you get downstream? Discover how to unlock upstream AAV analytics with #MassPhotometry in this upcoming webinar from Rentschler Biopharma and Cell and Gene Therapy Catapult. Rentschler’s Nathan Sweeney and the Catapult’s Amna Anwar will detail a method that uses #MassPhotometry and small-scale purification method to characterize samples during upstream processing in AAV manufacturing pipelines. Their work is part of an ongoing collaboration with Refeyn, supported by Innovate UK, that aims to develop and apply state-of-the-art analytical techniques to accelerate and enhance AAV manufacturing. Register for the webcast in the link below and check out the rest of the talks in the Viral Vectors Digital Week line-up from Bioprocess International, from December 2nd to 4th. 👉 https://lnkd.in/g3r6KM-p

#MassPhotometry can help you finalize your antibody stability analysis in record time, with consistent results and at multiple concentration ranges! Refeyn has released its new StreamlineMP software suite, that provides enhanced analysis workflows for specific applications. Its first module – the Antibody Stability Module – is tailored for the large datasets obtained in antibody aggregation analysis – reducing analysis times by over 80%.   Mass photometry usually works at the nanomolar concentration range, perfect for analyzing the contents of IV bags. If you want extra flexibility – or want a direct comparison to other analysis techniques like SEC – the MassFluidix HC add-on enables mass photometry measurements at the micromolar range, too.  Learn more about fast, in-process antibody analysis with mass photometry in our new tech note!  👉 https://lnkd.in/dksPZN6i

Introducing StreamlineMP! Our new software platform complements the speed of #MassPhotometry measurements with fast and consistent data analysis for specific workflows. 🔬 Beginning with our Antibody Stability Module, the platform offers specialized modules – supporting customers who need to repeatedly apply the same analysis to large datasets. The modules automate analysis, saving time and ensuring consistency. ⏱️🔄 The Antibody Stability Module enables fast, efficient analysis of the datasets obtained during antibody stability analysis, providing robust statistics on aggregation for replicate groups.  Learn more here! 👉 https://lnkd.in/gJ-tYU7f

This month, our Employee Spotlight 🤩 features Nathan Curry, Senior R&D Engineer. Today he tells us about his experience working at Refeyn! Want to join the team? Check out current job openings here ➡ https://lnkd.in/ekkAYkRX #refeyn #worktogether #biotechnology #massphotometry #career

Ready to turn up the heat 🔥 on your AAV analytics? Join our upcoming webinar! Eduard Ebberink will present a new toolset to rapidly quantify and analyze thermal stability across different AAV serotypes by combining nuclease treatment with mass photometry. Register here to gain new AAV stability insights with #MassPhotometry. 👉 https://lnkd.in/dCNkEWzK

📽️ #MeetThePortfolio - Refeyn 🔬 Led by CEO Gerry Mackay, Refeyn is redefining the molecular research landscape through mass photometry—a revolutionary technique for better measuring molecular mass, by simply shining light on single biomolecules. Refeyn's technology emerged from a breakthrough moment in 2014 when researchers from the University of Oxford discovered that a light microscope could measure individual proteins' mass. By 2018, the team had transformed this concept into a quantitative tool. Today, valued at over $250 million and with a global team of 150+, Refeyn’s instruments are empowering scientists worldwide, from pharmaceutical labs to academic research. #innovation #healthtech #lifesciences #oxford

Peter Eisenhut and colleagues from Takeda analyzed rAAV9 samples produced with two different transfection reagents: FectoVIR and Polyethylenimine. Biopotency assays found that rAAV9 samples produced with FectoVIR had higher potency. Mass-based assays like mass photometry and AUC measured the proportions of empty, full and partially filled capsids and were in excellent agreement (😉), but did not detect differences between transfection methods. On the other hand, sequencing showed that the proportion of full-length transgene loads was lower than expected from mass-based methods, and did find a difference between transfection reagents, in agreement with biopotency assays. Read the full study here! https://lnkd.in/dA4A-D_g #MassPhotometryStories #AAV 

We bring news from the cutting edge of upstream process analytics in AAV manufacturing, featuring #MassPhotometry, of course!🔬✨ In this online event, Rentschler Biopharma’s Nathan Sweeney and Cell and Gene Therapy Catapult’s Amna Anwar will present the latest findings from an ongoing project supported by InnovateUK.📊🔍 Tune in to learn about an innovative purification method coupled to mass photometry analysis that enables monitoring of empty/full AAV ratios during upstream processing with minimal time and sample consumption, addressing a significant challenge in AAV manufacturing. Register here! https://lnkd.in/g3r6KM-p