Vesiculab

Studying the biomolecular corona dynamics of extracellular vesicles: in their latest article, Angelo Musicò, Paolo Bergese at University of Brescia and collaborators developed a fluorescence correlation spectroscopy-based methodology to investigate biomolecular corona formation on EVs derived from human red blood cells and amniotic membrane mesenchymal stromal cells in human plasma https://lnkd.in/e5Nw7nk3 Their results revealed distinct corona dynamics between the two EV populations, and suggested that the biomolecular corona's dynamics are influenced by the EVs' cellular origin, highlighting an additional layer of heterogeneity. An article co-authored by Andrea Zendrini, Santiago Alberto Gimenez Reyes, Valentina Mangolini, Lucia Paolini, Miriam Romano, Andrea Papait, Antonietta Silini, Paolo Di Gianvincenzo, Arabella Neva, Marina Cretich†, Ornella Parolini, Camillo Almici, Sergio E. Moya and Annalisa Radeghieri #extracellularvesicles #exosomes #biomolecularcorona #nanoparticles #Vesiculab

Investigating extracellular vesicles in viscous formulations using interferometric light microscopy: in their latest work, Lucile Alexandre, Kelly Aubertin at Université Paris Cité and collaborators introduced a methodology leveraging nanoparticle tracking analysis via interferometric light microscopy (ILM) to assess both the concentration and size distribution of nanoparticles, as well as their interactions with the surrounding environment using a nanorheological approach https://lnkd.in/gcUpFHDS By analysing interference patterns, their method enabled precise nanoparticle tracking not only in aqueous solutions but also in complex, high-viscosity, or non-Newtonian media, making it particularly suitable for characterising EV formulations. An article co-authored by Anastasiia Dubrova, Aruna K., Estelle SURPLY, Christopher Ribes, Imane Boucenna, Florence Gazeau, Amanda Silva Brun and Stephanie Mangenot #extracellularvesicles #exosomes #LIM #nanoparticles #rheology #Vesiculab

Promoting angiogenesis through extracellular vesicle release: in their latest article, Alessandra Maria Testa, Roberta Angioni at Università degli Studi di Padova and collaborators investigated the angiogenic properties and molecular signature of EVs released by human microglia under hypoxia-reperfusion conditions. They assessed the angiogenic potential of these EVs through endothelial cell assays and a zebrafish xenograft model, with additional in vivo studies in a mouse model of ischemic stroke https://lnkd.in/eNuyrdzA The authors identified key proteins driving the pro-angiogenic functions of hypoxia-induced EVs. They demonstrated that hypEVs enhanced endothelial cell migration and tube formation in vitro, while in vivo, they promoted vessel sprouting in zebrafish and increased microvessel density in the perilesional area of ischemic stroke-affected mice. An article co-authored by Livia Vignozzi, Diana Corallo, Sanja Aveic, Antonella Viola and Manuela Allegra, PhD #extracellularvesicles #exosomes #angiogenesis #microglia #stroke #Vesiculab

Developing a method for preparing mRNA-LNPs with enhanced properties: in their recent article, Cong Geng, Chunlei Li at Hebei Medical University and collaborators employed a two-step tangential-flow filtration (TFF) method to enhance the properties of mRNA-LNPs during preparation. Their approach included an additional ethanol removal step before particle fusion https://lnkd.in/eA-kxA_v Their innovative technique produced mRNA-LNPs with larger particle sizes, a lower proportion of empty LNPs, improved storage stability (maintaining integrity for at least six months at 2–8 °C), enhanced in vitro transfection efficiency, and reduced distribution to the heart and bloodstream in vivo. An article co-authored by Kefan Zhou, Ying Yan, Chan Li, Beibei Ni, Jiangman Liu, Yeming Wang, Xiaoyan Zhang, Dazhuang Wang, Lu Lv, Yongchuan Zhou, Anhua Feng and Yajuan Wang #LNPs #mRNA #vaccines #therapeutics #stability #Vesiculab 

Demonstrating the anti-aging effect of MSC-derived extracellular vesicles in osteoarthritis: in their latest work, Jeremy Boulestreau, Daniele Noel at University of Montpellier and collaborators used EVs derived from human adipose tissue mesenchymal stem cells (ASC-EVs) in two osteoarthritis chondrocyte models of senescence: inflammaging induced by IL1β and DNA damage induced by etoposide https://lnkd.in/ePd4mTGs The authored demonstrated that ASC-EVs effectively reduced markers of cellular senescence, including a decrease in SA-β-Gal-positive cells, reduced accumulation of γH2AX nuclear foci, and suppressed secretion of senescence-associated secretory phenotype factors. An article co-authored by Marie Maumus, Giuliana Minani Bertolino and Christian Jorgensen #extracellularvesicles #exosomes #cellularsenescence #osteoarthritis #Vesiculab

Assessing astrocyte dysfunction in ischemia-induced astrocyte-derived extracellular vesicles: in their latest work, Tímea Forró, Rodica Bălas at the University of Medicine, Pharmacy, Science and Technology "G.E. Palade" of Târgu Mureș and collaborators aimed to investigate the post-ischemic behaviour of astrocytes by detecting and characterising two additional cargo proteins, AQP4 and GDNF, in EVs derived from these cells in the same patient group. Additionally, evaluated the potential of these EV proteins as biomarkers for assessing stroke severity and predicting outcomes https://lnkd.in/eGyd2AGN The authors concluded that the presence of AQP4 and GDNF in post-ischemic astrocyte-derived EV cargo underscores the significance of EV protein profiling in elucidating the astrocytic response to ischemia. An article co-authored by Doina Ramona Manu and Lucian Barbu #extracellularvesicles #exosomes #stroke #astrocytes #Vesiculab

Streamlining miRNA loading into extracellular vesicles: in their latest article, Corinna Torabi, SJ. Claire Hur at The Johns Hopkins University and collaborators developed a rapid and efficient method to load surface protein-specific EV subpopulations with miRNA using electroporation while the EVs are bound to antibody-coated beads. They subsequently demonstrated the selective capture of CD81+ EV subpopulations with magnetic microbeads, enabling swift EV manipulation, miRNA loading, and purification https://lnkd.in/eR_7QxvG Their approach reduces post-electroporation EV washing time by 20-fold compared to the conventional ultracentrifugation method, saving approximately 2.5 hours in removing unloaded miRNA while maintaining efficiency. An article co-authored by Sung-Eun Choi, Thomas R. Pisanic II, Ph.D. and Michael Paulaitis #extracellularvesicles #exosomes #bioengineering #miRNa #targeteddelivery #Vesiculab

Studying urinary extracellular vesicles and uromodulin: in their recent article, Mike Harding, Uta Erdbruegger at the University of Virginia highlighted structural features of uromodulin that make it resistant to separation from urinary EVs. They reviewed commonly used urine-EV enrichment techniques, their impact on uromodulin separation, and specific methods designed to remove uromodulin during urine-EV preparation https://lnkd.in/euEFqX_z The authors also discussed the importance of uromodulin removal in achieving accurate results for various research objectives. An article co-authored by Hayrettin Yavuz, Annika Gathmann, Samantha Upson and Agnieszka Swiatecka-Urban #extracellularvesicles #exosomes #uromodulin #EVisolation #Vesiculab

Delivering therapeutic RNA to the brain using extracellular vesicles: in this review article, Paniz Shirmast, Nigel McMillan at Griffith University and collaborators provided a comprehensive analysis of RNA delivery into the brain, with a strong focus on EV-mediated approaches. They discussed the types of RNA molecules being delivered and the significant challenges of crossing the blood-brain barrier https://lnkd.in/er8R6zBG They also highlighted innovative strategies to facilitate RNA transport into the brain and explored the potential of RNA delivery for treating neurological disorders, emphasising the transformative impact of EV-based methods. Additionally, they examined future directions and emerging technologies in this rapidly advancing field, offering valuable insights into the development of RNA therapeutics for neurological conditions. An article co-authored by Mahdi AbedinzadeShahri, Austin Brent and Adi Idris #extracellularvesicles #exosomes #LNPs #RNAtherapeutics #neurodegenerativedisease #Vesiculab

Reviewing the role of extracellular vesicles in viral liver diseases: in their latest article, Elias Kouroumalis, Ioannis Tsomidis at Medical School / University of Crete and Argyro Voumvouraki at AHEPA University Hospital highlighted the critical role of EVs in viral liver diseases, emphasizing their involvement in transporting viral proteins, various RNA types, and even complete virions. This transportation contributes to the pathogenesis of conditions such as viral cirrhosis and virus-related hepatocellular carcinoma https://lnkd.in/gigEWnw7 They also discussed the biogenesis of EVs and their roles in disease progression, alongside their potential as diagnostic and prognostic biomarkers. #extracellularvesicles #exosomes #hepatitis #liverfibrosis #carcinoma #Vesiculab