Association for Single Cell Analysis (ASCA)

📍 Soft tissue sarcomas at the single-cell and spatial resolution Menyailo et al. first summarized single-cell sequencing and spatial transcriptomic studies of soft tissue sarcomas, rare malignancies with a high mortality rate.   Key points 🔸 Tumor and microenvironment cell populations 🔸 Pathogenetically significant genes 🔸 Clinically relevant markers 🔸 Therapeutically valuable molecular targets. ➡️ More details: https://lnkd.in/eCpvQxjY #spatialomics #spatialbiology #singlecellanalysis #singlecell

📍 SPACe: an open-source, single-cell analysis of Cell Painting data Stossi et al. introduce SPACe, an open-source platform for analysis of single-cell image-based morphological profiles produced by Cell Painting. Key points ✔️Processing speed, accuracy in mechanism of action recognition, and reproducibility across biological replicates ✔️Applicability to multiple models, sensitivity to variable cell-to-cell responses, and biological interpretability to explain image-based features ✔️Defined screening campaign of cell metabolism small-molecule inhibitors tested in seven cell lines. ➡️ More details: https://lnkd.in/eTCyPQcD #BaylorCollegeofMedicine #spatialomics #spatialbiology #singlecellanalysis #singlecell

📍 Whole-brain spatial transcriptional analysis at cellular resolution    Kanatani et al. developed Tris buffer-mediated retention of in situ hybridization chain reaction signal in cleared organs (TRISCO), an effective method for whole-brain spatial three-dimensional RNA imaging.     Key points  ✨The preservation of RNA integrity, achieving uniform RNA labeling, and enhancing tissue transparency  ✨Testing using a broad range of cell-identity markers, noncoding and activity-dependent RNAs, within diverse organs of varying sizes and species  ✨Monitoring the expression of immediate early genes after administration of the antiobesity drug semaglutide in mice. ➡️ More details: https://lnkd.in/dE3G7PbE #spatialomics #spatialbiology #singlecellanalysis #singlecell

📍 The Human Cell Atlas: towards a first draft atlas In the recent Nature collection, the Human Cell Atlas consortium presents tools, data and ideas towards the generation of their first draft atlas of cells in the human body. Collection content ✅Overview of the Human Cell Atlas ✅Developmental cell atlas ✅Computational tools for data analysis and integration ✅Organ-specific integration ➡️More details: https://lnkd.in/eaKD6iRY #spatialomics #spatialbiology #singlecellanalysis #singlecell

ASCA Moscow 2024. Speakers 📍Olga Efimova (Skolkovo Institute of Science and Technologies) shared her impressions👌 During the conference Olga presented a talk "Spatially resolved transcriptomics of newborn human prefrontal cortex".

💬 "Very good place to meet new people, to establish new collaborations and to share our results" 🎥 Yulia Medvedeva (Research Center of Biotechnology) presented a talk "Immune cells diversity in T2D" during the ASCA Moscow 2024.

💬 "ASCA helped me to do my first single-cell research" New video with Daria Bogdanova (Sirius University), who presented a talk "PPM1D phosphatase as a mediator of tumor-immune interactions".

💬 "Amazing event that brings a lot of people together" Dmitry Svetlichnyy (Centre for Strategic Planning of FMBA) shared impressions about ASCA Moscow 2024. Dmitry presented a talk about remodeling of the chromatin landscape in peripheral blood cells in patients with severe Delta COVID-19. 🎥

ASCA Moscow 2024. Speakers 📍Polina Vishnyakova (RUDN University) told us how single-cell techniques help in research, and also, what single cell analysis and berry cocktail have in common 🍓 🥤

📍 New insights into oogenesis in Crustaceans Lu et al. used single-nuclei RNA sequencing to develop an atlas of the ovary in the mud crab Scylla paramamosain, which is distributed in the Indo-Pacific region and has great economic value due to its high nutritional benefits.   Key findings 🔸 Five cell types, including germ cells, somatic cells, and three follicle cell types 🔸 The role of cell communication in oogenesis 🔸 Evolutionarily conserved transcription factors and key molecules/pathways relevant to oogenesis 🔸 The essential roles of piwi and VgR in oogenesis ➡️ More details: https://lnkd.in/eFYiuD7n #spatialomics #spatialbiology #singlecellanalysis #singlecell