Annie 🅰️’s Post

🧬 Scientists engineered genetic circuits to improve the productivity of industrial E. coli at low pH Low pH fermentation in industrial processes provides significant benefits like reducing the need for neutralizers and wastewater output, especially in producing amino acids and organic acids. The researchers created a quorum-sensing gene circuit that activates acid-resistance genes during exponential growth and deactivates them in stationary phases. This reduces unnecessary energy consumption, enabling E. coli strains to sustain high productivity in acidic conditions. The engineered E. coli strain produced 102 g/L of lysine at pH 5.5, with 4% less glucose consumption and 10% lower ammonia usage. This indicates improved production efficiency and sustainability through better resource use and reduced waste. ✉️ Hungry for more updates on biotech-enabled agrifood businesses and breakthroughs? Devour the free subscription: betterbioeconomy.com

Here to bring attention to Synthetic biology - my personal favorite in biology (though a multidisciplinary field in itself), and reason I became interested in biotech in the first place! When people refer to bioengineering- most of the time, it is part of synthetic biology. Synthetic biology has sky-rocketed within the past few years, being valuated at 15.47 billion in 2023 and estimated to go up to 100 billion dollars by 2030. BCG estimates that synthetic biology could play a significant role in manufacturing industries, potentially impacting over one-third of global production. Some of the cool inventions within synthetic biology: 1. Insulin production: Insulin used to be made though pigs pancreas, now most insulin given to patients, it made through an insulin gene into a plasmid, transferring the gene into bacteria to produce insulin (most commonly E.Coli) 2. Saffron via Yeast: Evolva, Companies like Evolva have bioengineered yeast to produce the key compounds found in saffron—a highly expensive spice. 3. Sustainable Palm oil: C16 Biosciences produces lab grown palm oil which is 20% cheaper, and environmentally friendly 4. Cancer fighting cells: : Researchers have engineered human immune cells to recognize and destroy cancer cells—paving the way for breakthroughs in cancer treatment.

Enhancing Skills in Molecular Biology and biotechnology Over the past 2-3 years, I have been honing my skills in various aspects of molecular biology and biotechnology. Here are some key areas where I've gained significant experience: Lab Techniques and Molecular Biology Practices : Hands-on experience with autoclave, laminar air flow, ph meter, aseptic handling, good lab practices, sample processing, DNA and RNA extraction, Protein estimation and many more and these skills has deepened my understanding and proficiency in molecular biotechnology and in plant biotechnology as well Micro propagation: Making millions of copies of plants using a single plant always fascinated me, through the use of biological tools and practices it is quite easy, less time and less space consuming process. Data collection: Data collection from various sources and using of primary and secondary data, participants engaging and ethnographic studies. Lab Handling: Efficiently managing lab environments, ensuring safety, and maintaining accurate records has been a crucial part of my daily routine. Digitization of Data: Transitioning to digital data management has streamlined our processes, making data more accessible and easier to analyze. Data writing and review writing: Crafting clear and concise data reports and reviews has enhanced my ability to communicate complex information effectively. These experiences have been instrumental in my professional growth, enabling me to contribute more effectively to our team's success. Need to work on many of the things, learning each and every thing step by step. How are you applying your skills in your current role? Let’s connect and share insights! Molecular Biotecnologia Plant Tissue Culture Market Bayer Crystal Crop Protection Limited Indiana Crop Improvement Association Crop Protection Plant Engineering Bharat Biotech IQVIA BioTecNika Biocon Biologics World Health Organization

Biotechnology involves the use of living organisms or biological systems to develop products and processes for various applications. Advanced equipment plays a crucial role in biotechnology research and development. Here are some examples of advanced equipment commonly used in biotechnology: 1. Polymerase Chain Reaction (PCR) machines: PCR machines are used to amplify specific DNA or RNA sequences, enabling the study and manipulation of genetic materials. 2. DNA/RNA sequencers: These instruments are used for sequencing DNA or RNA, allowing researchers to determine the precise order of nucleotides in a given genetic sequence. 3. Flow cytometers: Flow cytometers are used for analyzing and sorting cells based on their physical and chemical properties, enabling researchers to study cell populations and purify specific cell types. 4. Bioreactors: Bioreactors are vessels designed for the controlled growth of microorganisms or plant and animal cells, enabling the production of various biological products, such as pharmaceuticals, enzymes, or biofuels. 5. Protein purification systems: These systems, which often involve chromatography techniques, are used to purify and isolate specific proteins from complex mixtures. 6. Mass spectrometers: Mass spectrometers are used to analyze the mass and composition of molecules, including proteins, peptides, and other biomolecules, providing valuable information about their structure and function. 7. Microscopes: Advanced microscopes, such as confocal microscopes, electron microscopes, and atomic force microscopes, are used to visualize and study biological samples at high resolution and magnification. 8. Microarray systems: Microarray systems are used to analyze the expression levels of thousands of genes simultaneously, enabling researchers to study gene expression patterns and identify potential biomarkers. 9. Automated liquid handling systems: These systems are used for precise and high-throughput handling of liquid samples, enabling efficient and accurate preparation of samples for various analyses. 10. Bioinformatics software and hardware: Powerful computational resources, including high-performance computing clusters and specialized bioinformatics software, are essential for analyzing and interpreting large-scale biological data generated by advanced biotechnology equipment. These are just a few examples of the advanced equipment used in biotechnology. The specific equipment used may vary depending on the research area or application, such as pharmaceutical development, agricultural biotechnology, or environmental biotechnology.

Most Common Biotechnology Experiments Being a student of biotechnology I have been taught some basic experiments in laboratory and these all are revolving around preparation,extraction,purification,sterilization,dilutions and etc.These all vary according to fields of biotechnology such as good, industrial, agriculture, health biotechnology and many more So, in my point of view there are some common listed below Culture Media preparation methods Culture media preparation is the process of mixing nutrients, agents for buffering and maintaining the osmotic balance, as well as selective inhibitors or indicators to create an agar or broth that supports the growth and the differentiation of microorganisms Steak Plate method The streak plate method is a microbiological laboratory technique of isolating pure cultures, and/or getting well-isolated colonies of bacteria from a mixed population. It is mostly used to get pure cultures of bacteria however, yeasts can also be isolated by this method. It is one of the most commonly used aseptic techniques in microbiology to isolate and propagate bacteria DNA extraction methods DNA extraction is a method to purify DNA by using physical and/or chemical methods from a sample separating DNA from cell membranes, proteins, and other cellular components. Gel electrophoresis Gel electrophoresis is a laboratory method used to separate mixtures of DNA, RNA, or proteins according to molecular size. Polymerase chain reaction Polymerase chain reaction (PCR) is a laboratory technique used to amplify DNA sequences Tissue culture preparation Tissue culture is a technique in which fragments of plants are cultured and grown in a laboratory. Many times the organs are also used for tissue culture.

This is exceptional work on how biological complexity can be explored: one study using combinatorial genetic libraries and ML, and the other optimising genetic and process factors simultaneously with DOE. A glance at the abstracts (I must find time to read the papers properly) shows that two rounds of combinatorial library + ML gave a 68% increase in production, whereas 2 rounds of DOE gave a 168 fold increase (i.e. 16,800%!). This seems like an incredible difference. Edit: re-read the papers: it's 168 difference between responses across the design, not 168 fold improvement! Sara Moreno Paz, is it in any way fair to compare the two pieces of work in this way? And if there is some basis for the comparison, what do you think accounts for such a huge difference? Congrats on some great science.

Future in Biotechnology Biotechnology is a rapidly advancing field with a promising future full of exciting opportunities. Here are some key trends and advancements shaping the future in biotechnology: Gene Editing: Technologies like CRISPR-Cas9 are revolutionizing gene editing, allowing precise changes to be made to the DNA of living organisms. This has vast applications in areas such as medicine, agriculture, and biomanufacturing. Synthetic Biology: Scientists are designing and constructing new biological parts, devices, and systems to solve complex problems and create novel products. This field has the potential to create bio-based materials, chemicals, and fuels with improved sustainability and efficiency. Personalized Medicine: Biotechnology is enabling the development of customized medical treatments tailored to individual patients based on their genetic makeup. This approach can lead to more effective therapies with fewer side effects. Bioinformatics: With the massive amounts of biological data being generated, bioinformatics is essential for organizing, analyzing, and interpreting this information. Advances in this area are driving discoveries in genomics, proteomics, and drug development. Regenerative Medicine: Biotechnology is playing a key role in the development of regenerative therapies that aim to repair, replace, or regenerate damaged tissues and organs. Stem cell research, tissue engineering, and 3D bioprinting are all contributing to this field. Precision Agriculture: Biotechnology is improving agricultural practices by developing genetically modified crops that are more resistant to pests, diseases, and environmental stresses. This helps increase crop yields, reduce the use of pesticides, and enhance food security. Biofuels and Bioremediation: Biotechnology is being used to produce sustainable biofuels from renewable resources like algae, waste biomass, and agricultural residues. It is also applied in bioremediation efforts to clean up environmental pollution using biological agents. These are just a few examples of the exciting developments shaping the future of biotechnology. As technology continues to advance, the possibilities for innovation and discovery in this field are virtually limitless.