Biosensors MDPI’s Post

Exciting Collaboration and Breakthroughs in Food Proteomics Research🌱🔬 I'm thrilled to share the highlights of our latest research collaboration with Teagasc Food Research Centre, Ireland. Our paper, recently published in LWT - Food Science and Technology delves into innovative methods for enhancing pea protein extraction. Our study meticulously explored various cavitation technologies, including ultrasonic and hydrodynamic cavitation, to maximize protein recovery and maintain structural integrity. The proteins identified in our research encompass crucial families such as Legumin, Vicilin, Convicilin, Albumin, Lipoxygenase, and Heat shock proteins with high value for human food. Key findings:  📈 Highest Recovery Rates: The ultrasonic probe method achieved the highest protein recovery rate, significantly outperforming conventional techniques. 🔧 Structural Integrity: Cavitation technologies preserved the primary structure of pea proteins while altering secondary and tertiary structures, enhancing their functional properties. 🏭 Scalability: Hydrodynamic cavitation showed immense potential for industrial-scale applications, promising better yield and purity. This collaboration exemplifies the synergy between advanced technology and sustainable food processing, paving the way for eco-friendly and sustainable protein sources. 🌍✨ Read more about our findings and the future of protein extraction.  https://lnkd.in/d26f3TBx #ProteinResearch #SustainableFood #Innovation #Collaboration #FoodTechnology #Teagasc #AssociatedAssociatedAssociated Laboratory for Green Chemistry (LAQV/REQUIMTE) #PROTEOMICSNOVA

📢 𝗡𝗲𝘄 𝗥𝗲𝘃𝗶𝗲𝘄 𝘄𝗶𝘁𝗵𝗶𝗻 𝗘𝗨𝟰𝗠𝗢𝗙𝘀!🌟 📄 Check out our latest review: “Sweet MOFs”: Exploring the Potential and Restraints of Integrating Carbohydrates with Metal–Organic Frameworks for Biomedical Applications. 🔬This comprehensive study explores the fusion of MOFs and carbohydrate chemistry to create biocompatible systems for biomedical applications. With biodegradability, low toxicity, and high surface area, MOFs can be functionalized with biological ligands and loaded with active cargos—from small drugs to biomacromolecules. 💡 Covering the past five years of progress, this review examines both opportunities and challenges, including sustainability and real-world applications. 🔗Read the full article: https://lnkd.in/dTA8MH2G #MOFs #Carbohydrates #Biomedicine #MaterialsScience #COSTAction #SustainableMaterials #Biocompatibility

#analyticalchemistry news Exploring the intricate world of #chemistry, a new study published in *Analytical Methods* sheds light on the advanced trends in detecting boldenone, its metabolites, and precursors. Boldenone, a synthetic anabolic steroid, often used illegally to enhance performance in sports, poses significant challenges for detection due to its complex metabolism. Authored by a team of experts, this integrative review meticulously examines chromatographic methods for identifying boldenone in various biological matrices. Chromatography, a powerful analytical technique, plays a crucial role in the separation and analysis of complex mixtures, making it an essential tool for scientists striving to ensure integrity and compliance in sports and health sectors. The research meticulously evaluates diverse chromatographic techniques, providing insights into their effectiveness and limitations. By doing so, it not only advances scientific understanding but also contributes to the development of more precise and reliable testing protocols. For the general public, this advancement holds practical significance. Enhanced detection methods mean better protection against the unfair advantage of performance-enhancing drugs in sports, safeguarding the spirit of fair competition. Moreover, it enhances our ability to monitor and control the use of such substances, promoting overall health and safety. To gain deeper insight into the study and its implications, delve into the full article here: [Read more](https://lnkd.in/dJxBKtUu,. #AnalyticalMethods #Boldenone #Chromatography #Science #Innovation #Health #SportsIntegrity, If you want to know more about #analyticalchemistry news, follow me: https://lnkd.in/d29pbjb9

During heating of milk, distinct conformational changes of beta-lactoglobulin occur, which may change its allergenic properties. Early conformational changes were tracked regioselectively throughout the entire protein sequence by LCMS analysis of labeling kinetics. Please check out our new paper first authored by Jiajia Liu at https://lnkd.in/ej3DvUhK The study was part of International Doctoral Program "Receptor Dynamics: Emerging Paradigms for Novel Drugs" by Elitenetzwerk Bayern. #FAU #foodchemistry #Lebensmittelchemie

At Enzyan, we are captivated by the intricate molecular architectures that nature creates and their potential to address society's most pressing problems. This week we have an example on how enzymes can perform incredibly precise reactions. 🌱 Scopolamine is a potent alkaloid traditionally extracted from plants like Datura and Hyoscyamus. Scopolamine, a nonspecific muscarinic antagonist, plays a crucial role in medicine, particularly as a treatment for motion sickness, postoperative nausea, and certain neurological conditions. In fact, it is on the World Health Organization's List of Essential Medicines. However, the current reliance on plant extraction to obtain scopolamine presents challenges—it's labor-intensive, dependent on agricultural yields, and susceptible to environmental factors. 🧑🔬 At Enzyan, we believe biocatalysis offers transformative solutions. By harnessing the power of enzymes, we can rethink the biosynthesis of scopolamine, moving beyond traditional plant sources. Enzymes, much like chemical catalysts, can be employed in innovative manufacturing processes to introduce complex functional groups in an enantioselective fashion. As an example, the enzyme 6β-hydroxyhyoscyamine epoxidase is able to perform a late-stage regio and enantioselective epoxidation of a non activated C-H bond to yield the final product Scopolamine. This unique reaction shows the power of biocatalytic transformations. Through enzyme-driven biosynthesis, we can unlock more sustainable, scalable, and highly precise methods to produce scopolamine. 🤝 Let's connect if you're interested in exploring how biocatalysis can revolutionize the production of your target molecule! office@enzyan.com #alkaloids #biocatalysis #scopolamine #enzymes #C-Hactivation #latestagefunctionalization

The Benefits of Non-targeted Analysis for PFAS Characterization. A recent study published in 'Environmental Science & Technology' shows that non-targeted analysis allows for a more comprehensive characterization of PFAS in biological systems, which will improve exposure assessment and evaluation of associated health impacts. https://lnkd.in/eQipXDJi

🧪 Biofoundry: Your Partner for Elite Enzymes for Life Sciences 🧪 We know how critical enzyme performance is in life science research and further downstream manufacturing. That’s why Biofoundry offers high performance specialty enzymes designed for reliability and consistent performance under ISO 13485, batch after batch. Biofoundry’s Elite Enzymes Ensure: • 🎯 High Purity and Activity: Enhance your end product, delivering results with precision. • 🏭 Enzymes Tailored for Downstream Manufacturers: Our enzymes are formatted for manufacturing workflows in molecular biology, IVDs, and more. • ✅ Consistent Quality: Rely on our enzymes for reproducible results every time. Learn how we can help your manufacturing workflow—reach out to us at sales@biofoundry.com. #Biotechnology #Enzymes #ResearchTools #BiofoundryTechnologies #MolecularBiology #IVD #GMP #ISO #BulkEnzymes

The year of 2024 was an exiting year! In this year, a lot of work has been done and resulted in great outcomes. I am very excited to share with you one of my papers that has been published in Food Hydrocolloids. I hope you find it interesting. This paper is titled "Effect of Temperature, pH, and Calcium Phosphate Concentration on the Properties of Reassembled Casein Micelles." In this paper we focused on understanding how processing factors affect the properties of reassembled casein micelles (RCMs). We hope the insights from this study can not only be used to modulate the composition and structure of RCMs but also help understand how processing parameters influence the assembly of RCMs from novel sources, such as recombinant caseins. This study is a collaborative effort between Wageningen University and Eindhoven University of Technology. I would like to extend my thanks to my amazing co-authors Bence Fehér, Ilja Voets, Kasper Hettinga, and Etske Bijl for their support. If you find this interesting, you can find the full paper here https://lnkd.in/eEqRD9e6

The FractioMate, an analytical product for high throughput Effect Directed Analysis developed by the VU and brought to you together with Bruker in 2025!   How do you identify biologically active, toxic compounds in water? 💧🔬 Persistent, mobile, and toxic compounds (PMT) are increasingly threatening our drinking water resources. To safeguard life, we must address emerging contaminants like pharmaceuticals, endocrine-disrupting compounds, and perfluorinated compounds. These pose serious health and environmental risks, including DNA damage, developmental disruption, reproductive issues, and cancer. Effect-directed analysis (EDA) is a key method for testing the biological activities of these compounds. Though traditionally lengthy, involving activity monitoring, fractionation, repeated bioassay testing, and chemical analysis, there's a game-changer. Together with our partner #Bruker, the #AIMMS scientists Dr. Jeroen Kool and Prof. dr. Marja Lamoree (#Vrije Universiteit Amsterdam) brings the #FractioMate, which, when paired with high-resolution mass spectrometry, simplifies the traditionally complex, costly, and time-consuming studies. This cutting-edge solution enables robust, rapid, and sensitive analyses, boosting the identification of biologically active compounds to 5-10 samples per week and greatly enhancing the detection of toxic compounds. #EnvironmentalScience #WaterQuality #AnalyticalChemistry #SustainableSolutions #ResearchInnovation 👉 Watch the video to learn more!💡 https://lnkd.in/ecqkSD7q Photo: Right: dr. Jeroen Kool in front of two FractioMates. Left: prof. dr. Marja Lamoree in front of a Bruker TIMS TOF pro2 mass spectrometer. Jeroen and Marja developed and commercialized the FractioMate.

Seemingly continued success. The HepG2 cells continue to grow -- albeit slowly and in clumps. So, without jinxing anything -- so far, so good. But what was the key to success here? - A new lot of cells from a new supplier? - A new antibiotic-antimycotic (for the record, I hate culturing with antibiotics/antimycotics, and intend to wean the cells off of them as soon as possible)? - Foregoing the centrifugation step (and avoiding the shearing forces) when thawing the cryopreserved stock (instead electing to just dilute the heck out of DMSO with medium)? - Using freshly-boiled deionized water, cooled to 37 C, to thaw the cells (avoiding any water bath issues)? - Adding fresh medium on top of existing medium after 48 hours, rather than changing the medium and disturbing the cells? - Not disturbing the cells for the first 48 hours after seeding? - Using collagen-coated tissue culture flasks? - Spraying and sanitizing with so much alcohol that I almost got drunk from the fumes? - Wearing disposable surgical gowns when working in the biological safety cabinet? For now, all's well that ends well. With time running out, the challenge now will be to pull a meaningful, significant, reproducible rabbit out of my hat in VERY short order! #biochem #biochemistry #biochemical #biochemist #biochemists #BiochemistryResearch #CellCulture #HepG2 #insulin #InsulinResistance #InsulinSignaling #laboratory #labresearch #LaboratoryResearch #MammalianCellCulture #MetabolicBiochemistry #metabolism #research #science #stem