Enzyan Biocatalysis GmbH’s Post

Exciting news for the microbial biosurfactants community and congratulations to Frederick Haala for this achievement: his first paper has been accepted! 🎉📝 https://lnkd.in/erMUmCrT Our journey into the fascinating world of #microbial #biosurfactant production reaches another milestone with our latest publication. This time, we delve deeper into the wonders of the yeast-like fungus #Aureobasidium, our incredible production host. What began as an endeavor with undergrad students back in 2018 has now evolved into a dedicated research group passionately exploring the potential of this remarkable bug. But that's not all – our work doesn't stop there! Aureobasidium biosurfactants, also known as polyol lipids, play a pivotal role in our SPRIND - Bundesagentur für Sprunginnovationen project, Quantum Leap (in the circular biomanufacturing challenge, https://lnkd.in/eUZhQ55U), amplifying the impact of our discoveries beyond the laboratory. In his new study, Frederick has built upon our previous successes and engineered a minimal medium that unlocks high efficiency in polyol lipid production. With 40 g/L in #bioreactors, low titers are now a thing of the past. And if that’s not enough, our straight-forward downstreaming process makes scaling up to pilot production an achievable prospect (keep your eyes peeled for Fredericks next publication)! Join us as we celebrate this milestone and anticipate the promising future ahead – one where Aureobasidium biosurfactants make the jump to the chemical industry and drive the development of the #circular #bioeconomy. 🌱💡 #Science #Innovation #polyol_lipids

TLDR: - the native lipid environment is crucial for GCPR stability - Nanodiscs mimic or extract the lipid environment; detergents do not. - GCPRs in nanodiscs are stable at 37°C and show temperature-dependent behavior. GCPRs are not stable in detergent at 37°C.

New article with my colleagues in China on the effects of different kinds of salt ions on the properties of whey protein gels. By selecting different anion types, the molecular interactions between the proteins can be modulated, which means gels with different properties can be produced: Impact of Hofmeister anion type on the structural and mechanical properties of composite whey protein hydrogels Link: https://lnkd.in/e7ZGPxqx

Thrilled to share that my latest research has just been published in Macromolecules! 📚 Explore "Effect of Impurities on the Formation of End-Group Clusters in Natural Rubber: Phenylalanine Dipeptide as an Impurity Protein" for fascinating insights. 🌟 #Research #Science #Macromolecules #RubberIndustry #AcademicPublishing 📈🔬

Epitopes, tags, and dyes make it easy to keep a close eye on specific proteins throughout the research process, ensuring better detection, quantification, and visualization during essential steps of a project. ICL offers a variety of protein labels to help tackle many of the nuanced applications scientists encounter on a daily basis. Read more in our recent article: https://lnkd.in/gZvVZrzx #epitope #proteinlabel #proteintags #dyes #research #lifescience #antibodies #blog #article #biotechnology

Exciting Collaboration and Breakthroughs in Food Proteomics Research🌱🔬 I'm thrilled to share the highlights of our latest research collaboration with Teagasc Food Research Centre, Ireland. Our paper, recently published in LWT - Food Science and Technology delves into innovative methods for enhancing pea protein extraction. Our study meticulously explored various cavitation technologies, including ultrasonic and hydrodynamic cavitation, to maximize protein recovery and maintain structural integrity. The proteins identified in our research encompass crucial families such as Legumin, Vicilin, Convicilin, Albumin, Lipoxygenase, and Heat shock proteins with high value for human food. Key findings:  📈 Highest Recovery Rates: The ultrasonic probe method achieved the highest protein recovery rate, significantly outperforming conventional techniques. 🔧 Structural Integrity: Cavitation technologies preserved the primary structure of pea proteins while altering secondary and tertiary structures, enhancing their functional properties. 🏭 Scalability: Hydrodynamic cavitation showed immense potential for industrial-scale applications, promising better yield and purity. This collaboration exemplifies the synergy between advanced technology and sustainable food processing, paving the way for eco-friendly and sustainable protein sources. 🌍✨ Read more about our findings and the future of protein extraction.  https://lnkd.in/d26f3TBx #ProteinResearch #SustainableFood #Innovation #Collaboration #FoodTechnology #Teagasc #AssociatedAssociatedAssociated Laboratory for Green Chemistry (LAQV/REQUIMTE) #PROTEOMICSNOVA

#Perspective Molecularly Imprinting–Aptamer Techniques and Their Applications in Molecular Recognition by Qingqing Zhou, Zhigang Xu and Zhimin Liu https://lnkd.in/e_GWx9Pg MDPI Kunming University of Science and Technology #molecularlyimprintedpolymer #aptamer #molecularrecognition #openaccess #Abstract Molecular imprinting–aptamer techniques exhibit the advantages of molecular imprinting and aptamer technology. Hybrids of molecularly imprinted polymer–aptamer (MIP–aptamer) prepared by this technique have higher stability, binding affinity and superior selectivity than conventional molecularly imprinted polymers or aptamers. In recent years, molecular imprinting–aptamer technologies have attracted considerable interest for the selective recognition of target molecules in complex sample matrices and have been used in molecular recognition such as antibiotics, proteins, viruses and pesticides. This review introduced the development of molecular imprinting–aptamer-combining technologies and summarized the mechanism of MIP–aptamer formation. Meanwhile, we discussed the challenges in preparing MIP–aptamer. Finally, we summarized the application of MIP–aptamer to the molecular recognition in disease diagnosis, environmental analysis, food safety and other fields.

Enzyme substrates are essential for bacterial detection and diagnostics. In comparison to traditional methods, the use of synthetic enzyme substrate has revolutionized how we identify and quantify bacterial species across clinical, environmental, and food safety sectors. They offer rapid, specific, and cost-effective solutions. Our last blog post gives you a short introduction to synthetic enzyme substrates: https://lnkd.in/eiuFiKcj Want more? Download our recently launched Enzyme Substrates ebook that dives deep into chromogenic substrates, exploring their applications and benefits. At Biosynth, we're proud to be at the forefront of this innovation, offering a comprehensive range of chromogenic, fluorogenic, and bioluminescent substrates. From our Aldol® and AquaSpark® product lines to high-purity substrates for lysosomal storage disorders, we're empowering researchers worldwide. Ready to elevate your bacterial detection methods? Find out more about our product range today. #EnzymeSubstrates #BacterialDetection #Diagnostics #Biosynth #Biotechnology

I am very happy to announce that the first paper "Biologically Active Secondary Metabolites from White-Rot Fungi" from our group "Biotechnology and Bioengineering" (https://lnkd.in/dumSUXuu) with my supervisor Susana Rodriguez-Couto, has just been published in Frontiers in Chemistry journal (IF 5.5). Please click the link below to explore our review paper: https://lnkd.in/deVRcRHb #WhiteRotFungi #SecondaryMetabolites

In 2018, I read a very interesting Science paper in which it was found that the cytosolic nucleoside diphosphate-x hydrolase (NUDX1), instead of geraniol synthase, is the main enzyme for #geraniol (a signature #rose_aroma) biosynthesis in #roses. This #counterintuitive finding inspired me why not using the rose NUDX1 enzyme in our proprietary bacterial platform to produce geraniol. Indeed, it works perfectly well in bacteria. We achieved breakthrough in early 2022, when I presented in #Bioflavour conference in Frankfurt, our technology immediately attracted the attention of several key industrial players including BASF/Isobionics and Givaudan. However, we met challenges in developing the fermentation bioprocess as we found that low oxygen supply was beneficial (unexpectedly). After dozens of experiments, we finally introduced the redox potential-control novel #microaerobic #fermentation process, achieved the highest titre ever reported for monoterpene biosynthesis, up to 19 g/L in only 4 days. FYI, the microbial synthesis of monoterpenes is well known to be very challenging due to the high toxicity, high volatility and enzyme inefficiency. To date, most monoterpene titres reported are at 1-2 g/L or even mg/L levels. Very glad to share this study, which demonstrating 1) the power of #metabolic_engineering #synbio on #sustainable production of #flavours #fragrance, and 2) the significance of the design/development of novel biosynthetic route and novel bioprocess; 3) cruciality of teamwork and multidisciplinary integration. We wish to thank A*STAR Singapore Institute of Food and Biotechnology Innovation (SIFBI) Nic Lindley, Ee Lui Ang for the great support. We thank the YIRG funding agency. This achievement won’t be possible without the relentless effort of the team by Sudha Shukal (strain pathway design and optimisation) Leonard Ong (microaerobic fermentation), T Rehka (enzyme purification and characterisation) and Xixian Chen on insightful discussion on enzyme engineering.