CYANAGEN S.R.L.’s Post

🚀 Redefining Microbial Cell Lysis and Homogenization in Biomanufacturing with cRUSH! 🌱 Homogenization and cell lysis are pivotal for extracting recombinant proteins from microbial expression systems like E. coli and yeast. Traditionally, high-pressure homogenization systems have dominated the field, built from stainless steel and requiring extensive cleaning, sterilization, and infrastructure. These systems lead to significant capital expenses, space requirements, and potential cross-contamination risks between batches. 🔍 Many have tried alternatives, such as ultrasonic homogenizers, but these technologies also fall short: they are not single-use, lack scalability, and often generate heat pockets that can degrade the quality of sensitive proteins. 🌟 Enter cRUSH: AthemBio's innovative single-use, continuous flow homogenization technology. Unlike traditional high-pressure systems or ultrasonics, cRUSH uses an impact-based physics for homogenization. This unique approach eliminates the need for pressure drops or external cooling, as the process generates no significant heat. The benefits are game-changing: Single-use format: cRUSH is designed for fast turnaround times, eliminating the need for extensive cleaning and sterilization. This significantly reduces the risk of cross-contamination, streamlines operations, and cuts capital and infrastructure requirements compared to stainless steel technologies. Scalable and adaptable: cRUSH can be fine-tuned to meet different lysis /homogenization intensities, from gentle to thorough, making it highly versatile for various biomanufacturing needs. No external cooling needed: cRUSH systems maintain a stable temperature, protecting the integrity of your product and simplifying the operational setup. With cRUSH, we are not just addressing the limitations of existing technologies—we’re offering a faster, safer, and more efficient pathway to protein production. 💡 How do you see single-use, cRUSH systems transforming the future of biomanufacturing? We’d love to hear your thoughts and experiences with emerging homogenization technologies! Contact us at info@athembio.com www.athembio.com #Microbial #Biotechnology #Bioprocessing #ProteinEngineering #CellLysis #Homogenization #SingleUseTechnology #Innovation #ContinuousManufacturing #Biomanufacturing #AthemBio #Biopharma #Novel #Breakthrough #Technology #Recombinantproteins

TLDR: - the native lipid environment is crucial for GCPR stability - Nanodiscs mimic or extract the lipid environment; detergents do not. - GCPRs in nanodiscs are stable at 37°C and show temperature-dependent behavior. GCPRs are not stable in detergent at 37°C.

Collagen type I is the most common naturally occurring protein in the human body. Due to its #biocompatibility, it's often used to develop human therapies. Creating in-vitro models with collagen derived from animal-free sources allows for more accurate development of relevant human models. HumaDerm provides researchers with consistent and high-quality native human #collagen source by controlling the tissue source and the manufacturing process under #ISO13485 standards. It contains type I collagen from native human skin, which allows for the creation of true human #regenerativemedicine therapies. Learn more about the HumaDerm product family and how it applies to your research here: https://lnkd.in/ghyc3CYY #tissueengineering #3dcellculture #drugtesting #hydrogel #biomaterials #bioprinting

Happy to share the publication of our latest paper dealing with a mechanistic soft-sensor design for protein refolding processes using intrinsic fluorescence measurements. https://lnkd.in/eAKYEe27 The paper is the continuation of our previous work (https://lnkd.in/easTiv85), where we already showed the potential that intrinsic fluorescence spectra offer for real-time monitoring of inclusion body based refolding processes. In the current work, we used this knowledge to build a model-based observer to estimate the concentrations of the single protein folding states. Check it out! Use this share link to download a copy of the full paper: https://lnkd.in/eeqXDYAU Shoutout to the Co-Authors: Chika Linda Igwe, Florian Gisperg, Matthias Kierein, Eva Prada Brichtova, Oliver Spadiut #protein #refolding #observer #softsensor #modeling #biotechnology

Application of Bacterial-Derived Long Cellulose Nanofiber to Suspension Culture of Mammalian Cells as a Shear Protectant Nanofibrillated bacterial cellulose (NFBC) is a bio-compatible long-fiber nanocellulose produced by cellulose-synthesizing bacteria. It forms an entangled network structure in the suspension state, thereby imparting greater viscosity than conventional media additives. In this study, we examined its application as a shear protectant in the suspension culture of mammalian cells to mitigate hydrodynamic stress imposed on the cells. The media supplemented with hydroxypropyl cellulose-adsorbed NFBC (HP-NFBC) exhibited an increase in shear viscosity according to rheometric analysis, similar to FP003, a commercially available medium additive. Suspension culture of Chinese hamster ovary cells in HP-NFBC-containing media under a high stirring rate (120 rpm) demonstrated higher cell growth and lower cell death compared to those in the medium without additives and in FP003. https://lnkd.in/g2WtF--v #aspenalert #biotech #bioprocess

New article with my colleagues in China on the effects of different kinds of salt ions on the properties of whey protein gels. By selecting different anion types, the molecular interactions between the proteins can be modulated, which means gels with different properties can be produced: Impact of Hofmeister anion type on the structural and mechanical properties of composite whey protein hydrogels Link: https://lnkd.in/e7ZGPxqx

Checkout the COOMASSIE nano™ Protein Staining Solution — available now at FroggaBio! The next-gen protein staining solution offers faster detection and higher sensitivity without hazardous substances. 🧬🌿🔬 LEARN MORE: https://hubs.li/Q02B8pB20 COOMASSIE nano™, enhanced by nano-technology, is a ready-to-use protein staining solution for SDS-PAGE gels. Its next generation formula offers faster protein detection, higher sensitivity, and requires no destaining or washing steps. Safe and environmentally friendly, COOMASSIE nano™ is free from hazardous substances like methanol and acetic acid. Additionally, it is compatible with mass spectrophotometry, making it a versatile and essential tool for modern laboratories. #FroggaBio #COOMASSIEnano #ProteinStaining #Biotech #LifeSciences #NanoTechnology #ProteinResearch #LabSolutions #BioResearch #SDS_PAGE #Electrophoresis #MassSpectrometry #NextGenBiotech #LabEquipment #BioTechInnovation #SafeLab #EcoFriendly #HighSensitivity #FastDetection #ScienceTools #LabInnovation #ResearchTools #MolecularBiology #LabProducts #Proteomics #LabSafety #GreenScience #LabEfficiency #BioTechProducts #AdvancedBiotech #ScientificResearch #LabEssentials

At Enzyan, we are captivated by the intricate molecular architectures that nature creates and their potential to address society's most pressing problems. This week we have an example on how enzymes can perform incredibly precise reactions. 🌱 Scopolamine is a potent alkaloid traditionally extracted from plants like Datura and Hyoscyamus. Scopolamine, a nonspecific muscarinic antagonist, plays a crucial role in medicine, particularly as a treatment for motion sickness, postoperative nausea, and certain neurological conditions. In fact, it is on the World Health Organization's List of Essential Medicines. However, the current reliance on plant extraction to obtain scopolamine presents challenges—it's labor-intensive, dependent on agricultural yields, and susceptible to environmental factors. 🧑🔬 At Enzyan, we believe biocatalysis offers transformative solutions. By harnessing the power of enzymes, we can rethink the biosynthesis of scopolamine, moving beyond traditional plant sources. Enzymes, much like chemical catalysts, can be employed in innovative manufacturing processes to introduce complex functional groups in an enantioselective fashion. As an example, the enzyme 6β-hydroxyhyoscyamine epoxidase is able to perform a late-stage regio and enantioselective epoxidation of a non activated C-H bond to yield the final product Scopolamine. This unique reaction shows the power of biocatalytic transformations. Through enzyme-driven biosynthesis, we can unlock more sustainable, scalable, and highly precise methods to produce scopolamine. 🤝 Let's connect if you're interested in exploring how biocatalysis can revolutionize the production of your target molecule! office@enzyan.com #alkaloids #biocatalysis #scopolamine #enzymes #C-Hactivation #latestagefunctionalization

Enzyme Immobilization Creative BioMart provides a highly efficient way to go from cell extract or cell culture supernatant to a versatile and robust immobilized pure enzyme preparation. https://lnkd.in/g-vn4MT

🌟 Enhance Your Protein Research with Thermo Scientific™ Zeba™ Desalting Products! 🌟 Are you looking to achieve high-quality desalting and buffer exchange for your protein samples? Discover the efficiency and reliability of Zeba Desalting Products! 🔬 Why Choose Zeba Desalting Products? • High Recovery Rates: Achieve up to 95% protein recovery with minimal sample loss. • Rapid Processing: Complete your desalting process in minutes, saving valuable time. • Versatile Applications: Suitable for a wide range of proteins, peptides, and other biomolecules. • Convenient Formats: Available in spin, gravity, and 96-well plate formats to fit your workflow needs. 🚀 Benefits for Your Research: • Improved downstream analysis due to high-quality desalting. • Enhanced reproducibility and reliability in your experiments. • Simplified workflow with user-friendly protocols. Join countless researchers who trust Thermo Scientific™ Zeba™ Desalting Products for their critical experiments. Elevate your research and achieve exceptional results. 🔗 Learn more about Zeba™ Desalting Products: https://lnkd.in/d8x-bC-c Connect with us to discuss how Zeba Desalting Products can benefit your research projects! #ProteinResearch #Desalting #BufferExchange #ThermoScientific #ZebaDesalting #Biotech #LifeSciences #ResearchInnovation