Phage Hunters Training and Research Program’s Post

𝗕𝗮𝗰𝘁𝗲𝗿𝗶𝗮𝗹 𝗗𝗶𝗮𝗴𝗻𝗼𝘀𝘁𝗶𝗰𝘀 𝗶𝗻 𝗔𝗾𝘂𝗮𝗰𝘂𝗹𝘁𝘂𝗿𝗲 𝗠𝗮𝗿𝗸𝗲𝘁 𝐑𝐞𝐬𝐞𝐚𝐫𝐜𝐡 𝐑𝐞𝐩𝐨𝐫𝐭 (𝟮𝟬𝟮𝟰-𝟮𝟬𝟯𝟯) ✨ 𝐆𝐫𝐚𝐛 𝐚 𝐬𝐚𝐦𝐩𝐥𝐞 𝐏𝐃𝐅 : https://lnkd.in/eFWsrYwc Bacterial diagnostics in aquaculture is the process of identifying the presence of bacterial pathogens in water and aquatic animals, as well as determining the amount of bacteria present. This process is important for the aquaculture industry, as bacterial diseases can cause significant losses in production due to the death of fish or shellfish, or the inability to market the affected product. Diagnostics can help identify the pathogen and prevent the spread of the disease, as well as provide insight into the underlying causes of the infection. 👉 𝗥𝗲𝘀𝘁𝗿𝗮𝗶𝗻𝘁𝘀 & 𝗖𝗵𝗮𝗹𝗹𝗲𝗻𝗴𝗲𝘀 The key restraints and challenges in Bacterial Diagnostics in Aquaculture market include the high cost of diagnostic tests, lack of access to advanced technologies, and limited availability of trained personnel. Bacterial diagnostics in aquaculture is a very expensive process due to the high cost of diagnostic tests. These tests require sophisticated laboratory equipment, reagents, and other materials that are not readily available. The cost of these materials is high and can be a major barrier for aquaculture farmers who are trying to diagnose and treat bacterial diseases. Most aquaculture farmers are unable to access advanced technologies, such as real-time PCR, due to the high cost of the equipment and the lack of trained personnel. This limits the ability of aquaculture farmers to accurately diagnose bacterial diseases in a timely manner, leading to delayed treatment and potentially the spread of the disease. The lack of trained personnel is another major challenge in the bacterial diagnostics in aquaculture market. Diagnostic tests require skilled technicians to accurately interpret the results and provide treatment recommendations. However, there is a limited availability of trained personnel in the aquaculture industry, which can lead to inaccurate results and delayed treatment. 👉 𝗞𝗲𝘆 𝗣𝗹𝗮𝘆𝗲𝗿𝘀 Ziva Impex Pvt. Ltd Pvt Ltd(IN),  Aquatic Diagnostics Ltd(UK), MOLOGIC LTD.(UK),  Biogenuix Medsystems Pvt Ltd(IN),  Thermo Fisher Scientific(US),  LexaGene Life Sciences(US) #Aquaculture #BacterialDiagnostics #AquaticHealth #FishFarming #AquacultureResearch #MarineBiotechnology #SustainableAquaculture #AquacultureInnovation #AquacultureIndustry #WaterQuality #AquaticDiseaseManagement #FisheriesScience #VeterinaryDiagnostics #SeafoodProduction #HealthTech

Lets start to promote alternative compounds or product against pathogen to reduce AMR and enviromental friendly in our country. One of those is herbal extracts along the abundance of herbal materials in Indonesia

Tackling Antimicrobial Resistance in Aquaculture: A One Health Approach Antimicrobial resistance (AMR) poses a significant challenge in aquaculture, with the misuse of antimicrobials potentially leading to the emergence of antibiotic-resistant bacteria. These resistant strains can be transmitted to humans through food, creating a public health risk that underscores the need for effective risk mitigation strategies. A recent study titled Antimicrobial Resistance in Aquaculture: Risk Mitigation within the One Health Context which was published in the journal Foods, provides a comprehensive overview of strategies to address AMR in aquaculture. The study emphasizes the interconnectedness of aquatic environments, intensive fish farming systems, the food processing industry, and human populations, all of which contribute to the spread of resistant bacteria. Key strategies for mitigating AMR, based on the One Health concept, include: 🔹 Stringent Antimicrobial Use Guidelines: Implementing strict guidelines to ensure antimicrobials are used judiciously and only when necessary. 🔹 Enhanced Disease Control: Promoting biosecurity measures, vaccinations, and alternatives to antibiotics, such as prebiotics, probiotics, immunostimulants, essential oils, peptides, and phage therapy. 🔹 Improved Practices: Focusing on better feeding practices, genetics, water quality monitoring, and wastewater treatment to prevent the development and spread of resistant bacteria. 🔹 Environmental Considerations: Addressing the environmental aspects of AMR, which are often overlooked, is crucial. Integrating environmental factors into AMR control efforts can help predict, detect, and prevent health risks associated with resistant bacteria. The study highlights the success of integrated AMR control strategies in Asia, where resistance to antimicrobial compounds has significantly decreased over the past two decades. For low and middle-income countries, aligning national AMR action plans with aquaculture practices is essential for effective risk management. Adopting a One Health approach in aquaculture not only enhances disease management but also contributes to a more sustainable and resilient aquaculture industry. By focusing on comprehensive risk mitigation strategies, we can reduce the prevalence of AMR and safeguard both aquatic and human health. Read the original article here: https://lnkd.in/gUSHBenP

𝗧𝗵𝗲 𝗣𝗼𝘁𝗲𝗻𝘁𝗶𝗮𝗹 𝗼𝗳 𝗣𝗵𝗮𝗴𝗲𝘀 𝗶𝗻 𝗔𝗾𝘂𝗮𝗰𝘂𝗹𝘁𝘂𝗿𝗲 Aquaculture is one of the fastest-growing food production sectors globally. In 2018, it accounted for 54.3% of total aquatic organism production. But this growth isn't without its hurdles, between 2008 and 2018, 67 different antibiotics were used in just 11 of the world's leading aquaculture-producing countries. This overuse has resulted in the emergence of resistant pathogens, rendering many treatments ineffective. (Le et al.,2018) 𝗠𝗲𝘁𝗵𝗼𝗱𝘀 𝗼𝗳 𝗔𝗱𝗺𝗶𝗻𝗶𝘀𝘁𝗲𝗿𝗶𝗻𝗴 𝗕𝗮𝗰𝘁𝗲𝗿𝗶𝗼𝗽𝗵𝗮𝗴𝗲𝘀 𝗶𝗻 𝗔𝗾𝘂𝗮𝗰𝘂𝗹𝘁𝘂𝗿𝗲 Bacteriophages can be administered through various methods: a) 𝗢𝗿𝗮𝗹𝗹𝘆: Phages are combined with feed effectively targeting gastrointestinal tract (GIT) infections. Through bacterial translocation, it may be possible to treat systemic infections. Jun et al. (2018) achieved a 50% survival rate in white shrimp using phage-treated feed. b) 𝗣𝗮𝗿𝗲𝗻𝘁𝗲𝗿𝗮𝗹𝗹𝘆 (intramuscularly, subcutaneously, intraperitoneally); it provides precise dosing but it's labour-intensive and not practical with small fish and crustaceans. Prasad et al. (2011) achieved 80% survival in Labeo rohita fingerlings with intramuscular phage injection against Aeromonas hydrophila c) In water baths/immersions; most used due to ease of application. The phage enters through the gills, potentially reaching internal organs and cleaning the environment as an added advantage. Zhang et al. (2014) successfully treated sea cucumbers infected with Vibrio alginolyticus using phage baths d) 𝗗𝗶𝗿𝗲𝗰𝘁 𝗿𝗲𝗹𝗲𝗮𝘀𝗲 𝗶𝗻𝘁𝗼 𝘄𝗮𝘁𝗲𝗿 𝘀𝘆𝘀𝘁𝗲𝗺𝘀; suitable for largescale operations and can treat the entire aquatic environment. Kalatzis et al. (2016) successfully reduced Vibrio alginolyticus in live fish feed cultures using this method 𝗜𝗻 𝗩𝗶𝘃𝗼 𝗨𝘀𝗲 𝗼𝗳 𝗕𝗮𝗰𝘁𝗲𝗿𝗶𝗼𝗽𝗵𝗮𝗴𝗲𝘀 𝗶𝗻 𝗔𝗾𝘂𝗮𝗰𝘂𝗹𝘁𝘂𝗿𝗲 The effectiveness of phage therapy in aquaculture varies, but many studies show promising outcomes: results: —Some experiments achieved 100% bacterial removal and zero mortality —Prophylactic use of bacteriophages has shown increased survival rates in various species. Schulz et al. (2019) achieved 16% higher survival in European eels when phages were administered a day before Vibrio vulnificus infection —In some cases, bacteriophage therapy outperformed antibiotic treatment. Vinod et al. (2006) achieved 46% higher survival in shrimp using phages compared to antibiotic treatment against natural Vibrio harveyi infection —Phages can persist in the environment, providing ongoing protection. Prasad et al. (2011) observed phages in fish organs and rearing water up to 10 days post-treatment Summarized by Elkana Mudi. 𝗦𝗼𝘂𝗿𝗰𝗲 Schulz P, Pajdak-Czaus J, Siwicki AK. In Vivo Bacteriophages’ Application for the Prevention and Therapy of Aquaculture Animals–Chosen Aspects. Animals. 2022; 12(10):1233. https://lnkd.in/dcR6BtMB #PhageResearch #PhageTherapy

🚀 Exciting advancements in aquaculture! Following on from our recent article in The Fish Site (https://lnkd.in/d8u95tt4), its exciting to see the promising work from the Fish-AI project, which developed an innovative artificial intestine platform to support preliminary dietary rankings ahead of live animal trials. Here are three key highlights: ⚗ Cutting-edge Technology: The platform uses fish intestinal cell lines and a dual-chamber system to simulate the fish gut, enabling comprehensive analysis of nutrient absorption and intestinal health. ♻ Sustainability Focus: By providing a preliminary ranking of diets without live animal trials, it saves time and resources, promoting sustainable aquaculture practices. 👩🔬👨🔬 Collaborative Effort: This groundbreaking project is a collaboration between top universities and companies, showcasing the power of teamwork in scientific innovation. The closing out presentation of the outcomes of the project is scheduled for the 26th November in Norway. If, like us, you cannot attend in person, there will be a live streaming of the event - check it out below 👇😊 #Aquaculture #Innovation #Sustainability #FishAI #Research #Aquafeed #Fishfarming #Nutrition https://lnkd.in/deeJ8HRW

New article about #antibiotic regulations in #aquaculture!

I would like to announce the publication of a recent review titled "Application and Prospect of Irradiation for Fish Health Management."  Prepared by me and my colleagues https://lnkd.in/gr-GrDhZ This review explores the innovative use of irradiation techniques in aquaculture, highlighting their potential benefits for enhancing fish health, improving growth rates, and controlling diseases. As the aquaculture industry faces numerous challenges, including environmental changes and infectious diseases, this research provides valuable insights into how irradiation can serve as a promising solution. I invite you to read the review to discover how these advancements can contribute to sustainable practices in fish farming. Feel free to share your thoughts or questions in the comments! #Aquaculture #FishHealth #Irradiation #Sustainability #Research #Innovation #Immunology #AquacultureNutrition

The potential of actinobacteria to improve health in aquaculture 🐟🔬 Disease outbreaks due to various factors are major concern in aquaculture. Most aquatic pathogens are opportunistic and any imbalance in the host-pathogen-environment triad will result in a disease outbreak. There is a constant search for new therapies, such as natural microbial strains that can be used as beneficial probiotics and bioaugmentants in culture systems.   Within this area, various studies have demonstrated the probiotic and bioremediation potential of several species of Actinobacteria that can be applied in aquaculture. Actinobacteria are Gram-positive, filamentous bacteria and represent one of the largest groups. They are widely distributed in terrestrial, freshwater and marine habitats.   Actinobacteria, especially Streptomyces, can be applied for disease prevention, treatment and bioremediation of organic and inorganic wastes in culture systems. Growth, immunity and resistance to aquatic pathogens in cultured organisms are also improved thanks to their ability to release potent antimicrobial compounds, bioactive molecules and new enzymes. Its broad-spectrum antimicrobial and quorum quenching activity can be well exploited against aquatic pathogens that form quorum sensing biofilms.   In the article “Applications of Actinobacteria in aquaculture: prospects and challenges” –carried out by a team of researchers from India– the applications, challenges and perspectives are analyzed precisely of actinobacteria in aquaculture. Research gaps are also highlighted which, if cleared, should pave the way for better understanding and utilization in aquaculture. #actinobacteria #bacteria #aquaculture #health #fishhealth #ram   Source: James, G., Prasannan Geetha, P., Thavarool Puthiyedathu, S. et al. Applications of Actinobacteria in aquaculture: prospects and challenges. 3 Biotech 13, 42 (2023). https://lnkd.in/ecnr7WSn

Title ISOLATION, PHENOTYPIC AND GENOTYPIC CHARACTERIZATION OF MULTI-DRUG RESISTANT ENTEROBACTER CLOACAE FROM DISEASED AFRICAN CATFISH IN LAGOS STATE Document Type : Research articleAuthorsOLAYINKA REMILEKUN ANIFOWOSE 1 Adebola O Ajiboye 2 Tolulope A Olakojo 31 DEPARTMENT OF VETERINARY MEDICINE, UNIVERSITY OF IBADAN2 Department of Aquaculture and Fisheries Management3 DEPARTMENT OF VETERINARY PHARMACOLOGY AND TOXICOLOGY, UNIVERSITY OF I UNIVERSITY OF IBADANDoi: 10.21608/svu.2024.328893.1348AbstractDisease outbreaks cause enormous financial losses and pose significant challenges to the aquaculture industry globally. This may lead to increased mortality rates, slower growth and productivity, greater production costs, and fewer revenue streams. The purpose of this study was to ascertain the prevalence and pattern of antibiotic susceptibility of Enterobacter cloacae isolated from diseased African catfish (Clarias gariepinus) in Lagos State, Nigeria. Aquaculture farms in three local government districts in Lagos State were the subject of this cross-sectional study. In Lagos State, 13 commercial farms located in three local governments (Surulere, Ikorodu, and Alimosho) provided a purposeful sample of 120 diseased African catfish (Clarias gariepinus). The 16S rRNA gene characterisation, biochemical testing, and morphological traits were used to identify the isolates. Using the agar disc diffusion method, the antimicrobial susceptibility pattern of Enterobacter cloacae was investigated. In infected Clarias gariepinus from Lagos State, the incidence of Enterobacter cloacae isolates was 60% (12/20) in Surulere, 76.7% (46/60) in Ikorodu, and 65% (26/40) in Alimosho. When comparing Ikorodu to other local governments, a noticeably greater prevalence was noted. Malaysian, Vietnamese, and Pakistani isolates of Enterobacter cloacae were closely related to the strains of OP595768.1 and OP595746.1 that were isolated. With a MAR value of 0.72, the isolates of Enterobacter cloacae were multidrug resistant. A study conducted in Lagos State revealed that Enterobacter cloacae, which was isolated from infected Clarias gariepinus, had evolutionary ancestry with harmful NCBI reference strains from various nations https://lnkd.in/etm7irWP

We are glad to share our newest review paper on AMR in aquaculture and available risk mitigation options within the One Health Context. Digest The application of antimicrobials in aquaculture primarily aims to prevent and treat bacterial infections in fish, but their inappropriate use may result in the emergence of zoonotic antibiotic-resistant bacteria and subsequent transmission of resistant strains to humans via food consumption. Aquatic environment serves as a potential reservoir for resistant bacteria providing an ideal breeding ground for development of antimicrobial resistance (AMR). The mutual inter-connection of intensive fish farming systems with terrestrial environments, food processing industry and human population creates pathways for the transmission of resistant bacteria, exacerbating the problem further. The aim of this study was to provide the overview of the most effective and available risk mitigation strategies to tackle AMR in aquaculture based on One Health (OH) concept. The stringent antimicrobials` use guidelines, promoting disease control methods like enhanced farm biosecurity measures and vaccinations, alternatives to antibiotics (AB) (prebiotics, probiotics, immunostimulants, essential oils (EOs), peptides and phage therapy), feeding practices, genetics, monitoring water quality, and improving wastewater treatment, rather than applying exessive use of antimicrobials, can effectively prevent development of AMR and release of resistant bacteria into the environment and food. The contribution of environment to AMR development traditionally receives less attention and therefore, environmental aspects should be included more prominently in OH efforts to predict, detect and prevent the risks to health. This is of particular importance for Low and Middle-income countries with the lack of integration of the national AMR action plans (NAP) with aquaculture-producing environment.   Integrated control of AMR in fisheries based on OH approach, can contribute to substantial decrease of resistance, such is the case in Asia, where in aquaculture, the percentage of antimicrobial compounds with resistance exceeding 50% (P50) decreased from 52% to 22% within the period of previous two decades. #WOAH #FAO #WHO #EFSA #ECDC #FSA #FDA #MDPIOpenAccess