Quotient Sciences’ Post

Hi, there! Have a look at my new article, which has been just published in the Biomolecules (MDPI, Q1) through the link given below:

Hi everyone! my first author paper is finally out now! I hope you enjoy reading it and get new insight into the world of phase separation!

"𝐓𝐫𝐚𝐧𝐬𝐟𝐞𝐜𝐭𝐢𝐨𝐧 𝐢𝐦𝐩𝐫𝐨𝐯𝐞𝐦𝐞𝐧𝐭 𝐛𝐲 𝐨𝐫𝐝𝐞𝐫𝐬 𝐨𝐟 𝐦𝐚𝐠𝐧𝐢𝐭𝐮𝐝𝐞" Thats catchy - here is a rundown: 𝐈 𝐚𝐦 𝐬𝐭𝐢𝐥𝐥 𝐝𝐢𝐠𝐞𝐬𝐭𝐢𝐧𝐠 𝐭𝐡𝐢𝐬 𝐩𝐫𝐞𝐩𝐫𝐢𝐧𝐭. 𝐖𝐨𝐮𝐥𝐝 𝐦𝐮𝐥𝐭𝐢-𝐬𝐭𝐚𝐠𝐞 𝐦𝐢𝐱𝐢𝐧𝐠 + 𝐜𝐆𝐀𝐒-𝐒𝐓𝐈𝐍𝐆 𝐢𝐧𝐡𝐢𝐛𝐢𝐭𝐢𝐨𝐧 𝐫𝐞𝐬𝐨𝐥𝐯𝐞 #LNP/ #pDNA 𝐭𝐨𝐱𝐢𝐜𝐢𝐭𝐲 𝐚𝐧𝐝 𝐥𝐨𝐰 𝐭𝐫𝐚𝐧𝐬𝐟𝐞𝐜𝐭𝐢𝐨𝐧 𝐞𝐟𝐟𝐢𝐜𝐢𝐞𝐧𝐜𝐲? Have a look for yourself - the team from University of Pennsylvania Perelman School of Medicine around Jacob S. Brenner and Zhicheng Wang took several approaches to improve on the outcome of pDNA/LNP transfection. Here is a link to the PDF: https://lnkd.in/d4r2X7Ur Two companies coming to mind that use 𝐃𝐍𝐀 𝐚𝐬 𝐭𝐡𝐞 𝐋𝐍𝐏 𝐜𝐚𝐫𝐠𝐨 are Generation Bio and Rampart Bioscience - I'd be intersted to see if they gave this new mixing method a shot: ➡️with or without the 𝐒𝐓𝐈𝐍𝐆 inhibitor ➡️with or without 4 𝐦𝐌 𝐌𝐠𝐂𝐥2, where Mg2+ enables compactation of the DNA ➡️with or without the 𝐛𝐮𝐟𝐟𝐞𝐫 𝐚𝐝𝐣𝐮𝐬𝐭𝐦𝐞𝐧𝐭𝐬 𝐝𝐮𝐫𝐢𝐧𝐠 𝐦𝐢𝐱𝐢𝐧𝐠 (citrate vs TRIS-based buffers) Congrats to all authors for pulling this together: Jia Nong, Xijing Gong, Quang Minh Dang, Sachchidanand Tiwari, Ph.D., Manthan Patel, Jichuan Wu, Andrew Hanna, Wook-Jin Park, Elena N. Atochina-Vasserman, Hui-Ting Huang, Oscar A. Marcos-Contreras, , Morris-Blanco, PhD, Jonathan J. Miner, Drew Weissman, Vladimir Muzykantov, Kushol Gupta, David Issadore, Jacob Myerson, Zhicheng Wang, Jacob S. Brenner

I'm thrilled to share our latest bioconjugation paper, helmed by Andres Rocha Tapia and Fabrice Abgottspon, now published in Chemical Science (one of my favourite journals!). Andres and Fabrice were fantastic throughout this project and I could not have asked anymore of them. I feel very privileged to have worked with them on this and know the work would not have been possible without them. This work demonstrates the superiority of site-directed conjugation approaches vs traditional non-selective chemistries for constructing affinity protein–ssDNA conjugates. We show that conjugates constructed from the site-selective chemistries display improved specific target binding and decreased non-specific binding, including for cell membrane targets. We hope this work can convince others to take a more considered approach when constructing affinity protein–ssDNA conjugates, particularly for analytical applications such as immunoPCR and proximity ligation/extension assays (PLA/PEA). I want to take this opportunity to thank Per-Åke Nygren and Johan Nilvebrant for their help with the SPR, and Vijay Chudasama for help on the bioconjugation. Our extended discussions were instrumental in getting this project off the ground. I would also like to thank Ioanna Thanassi, Peter Szijj, Andres Javier Bello Hernandez, Ghali Sekkat, François Cuenot, Sarah DUCLOS IVETICH, Nicola Aceto, and, of course, Andrew deMello, for making this possible. https://lnkd.in/dmUTzaTW

The cover art of the Nov. 2024 cover of JBC (ASBMB Journals) illustrates the degradation of deltamethrin, a pyrethroid insecticide, by the newly identified Cub and Sushi Domain-containing Insecticide Resistance (CSDIR) protein in Anopheles stephensi. This membrane-associated CSDIR protein (depicted in silver and orange) binds to and metabolizes deltamethrin (lemon-colored) extracellularly. Unveiled through SPR and HPLC analysis, this discovery offers critical insights into previously uncharacterized molecular pathways of insecticide resistance. ➡️Article by Kumar et al.: https://ow.ly/6O6c50UlbJt. ➡️Explore the issue: https://ow.ly/4aOS50UlbJv. 🎨Image credit: jatin kumar

The shortened warming protocol, such as the ultra-fast warming and single-step warming, has been recently drawing attention.   In this in vitro experimental study, shortened warming was demonstrated to induce over-rehydration, which might be associated with increased blastocyst-cell necrosis. Furthermore, supplementing warming solutions with fatty acids (FAs) significantly improved blastocyst outgrowth compared with non-supplemented solutions when the conventional protocol was used. However, when the shortened protocol was used, blastocyst outgrowth was comparable between the FA-supplemented and non-supplemented solutions; therefore, the conventional protocol is recommended in laboratories that use FA-supplemented solutions..   Some embryology laboratories have less personnel than that recommended in the guidelines. In these cases, the shortened protocol effectively improves the daily workflow. However, each laboratory should thoroughly examine the impact of the protocol before introducing it to prioritize the clinical outcomes.

𝐃𝐫𝐮𝐠𝐃𝐨𝐦𝐚𝐢𝐧, a database of interactions between ECOD domains of human target proteins and DrugBank molecules "DrugDomain describes the interaction of 5,160 DrugBank molecules, associated with 2,573 human proteins. It describes domains for all PDB structures of these proteins, and incorporates AF2 models when such structures are unavailable." Paper -> https://lnkd.in/g-m3zibm Database -> https://lnkd.in/gfxmKkec

Don't miss out on the July Nucleic Acid Insights issue! In this month's edition, we explore nonclinical tools and technologies being employed in the clinical translation of #mRNAtherapeutics, and investigate the key challenge of enhancing the stability of #nucleicaciddrugs in vivo. View below:

🔬 Interested in EV research? Check out our recent study in ACS Sensors highlighting the importance of upstream handling! First author Lucile Alexandre: "To analyze extracellular vesicles (EVs), preservation, extraction, and often preconcentration steps are crucial. In this study, we compared plasma preservation agents and evaluated EV extraction methods for integrity, size distribution, concentration, purity, and protein expression profiles, as well as factors like throughput, time-to-result, and cost. We found that the choice of anticoagulants depends on the extraction method and protein profiles highlighted the extraction of different EV subpopulations across methods. Our results underscore the significance of considering preanalytical parameters in EV research to account for their diversity and clinical utility as biomarkers. One aspect I truly appreciated about this project was the collaboration among multiple members of our team, each contributing their expertise, ranging from cell culture and microfluidics to blood collection, to collectively advance our understanding of extracellular vesicles." Advanced online publication available in ACS Sensors: 🔗 https://lnkd.in/en95hR_q #researchpaper #sciencenews #extracellularvesicles

Unveil a new technical article from the laboratory of Jeffrey Savas, PhD, on protein turnover in rodents via stable isotope metabolic labeling. Using isotope-enriched chow, such as 15N spirulina or 13C6 L-lysine, as the labeling source coupled with peptide-based LC-MS/MS analysis of tissue, physiological insights of protein dynamics can be quantitatively interrogated. Download the tech note for insights on experimental considerations, implementation, and application. https://lnkd.in/gMWuuYq8 Cambridge Isotope Laboratories, Inc #proteinturnover #stableisotope #metaboliclabeling