Snigdha Pramanik’s Post

Researchers have used #CRISPRCas9 for genome-wide screening of the parasite Toxoplasma gondii.   Apicomplexan parasites, such as Toxoplasma gondii, cause some of the most prevalent infectious diseases like #malaria and #toxoplasmosis, yet research into these pathogens has been limited due to traditional gene disruption methods not readily scalable for the whole genome.   The paper presents a CRISPR-based approach introducing a library of guide RNAs into parasites that constitutively express Cas9, enabling the identification of functionally important genes. Using this method, genome-wide screening and analysis can be completed in three weeks.   Read more in Nature Portfolio: https://shorturl.at/NYW7O

I bet many in the biology field would make great boxers—we’re always knocking out genes! 🧬🥊 And if you're the boxer, EDITGENE is definitely the best pair of gloves you can wear. Our customized stable knockout cell line service is equipped with an upgraded CRISPR/Cas9 system, giving you the precision and power you need to land that perfect KO. 💥 With over 1000 gene editing CRO projects completed and expertise in editing more than 300 cell types, we guarantee knockout success. Additionally, our knockout cell collection supports drug target research by providing rapid and reliable tools for both basic disease studies and drug development. Whether you're looking for specialized knockout strategies or ready-to-use cell line options, EDITGENE provides the knockout expertise to elevate your research. 🎯 Ready to Land the Perfect KO? Let’s hit hard together! https://lnkd.in/gaBQD95g #GeneEditing #CRISPR #Knock_In #KnockOut #PointMutation #Overexpression #CellLineDevelopment #GeneticResearch #PrecisionMedicine #BiotechInnovation #CustomCellLines #MolecularBiology #ResearchTools #Genomics #EDITGENE #CrisprLibraryScreening

In today’s fast-paced world, PCR is more than just a laboratory technique—it’s a driving force behind countless breakthroughs across healthcare, research, and biotechnology. From diagnosing diseases like COVID-19 to revolutionizing cancer research, PCR is transforming the way we understand genetics, treat illnesses, and improve public health. This powerful tool amplifies DNA with precision, allowing scientists to detect genetic mutations, identify pathogens, and even trace the origins of viruses. #PCR #MolecularBiology #Biotechnology #Genetics #HealthcareInnovation #CancerResearch #AgriculturalTech #BiotechRevolution #PersonalizedMedicine #DNA #GenomicScience #PublicHealth #TechForGood #zygene #zygenebiotechnologies

I'm thrilled to announce my fourth publication related to "Plant derived Bioactive compounds in Human health and Disease" 🌟,by contributing in the chapter 4 related to mechanism of phytochemicals in modulating signalling pathways involved in Inflammation and Cancer. 💜 link for the publication: https://lnkd.in/gN9rMaSZ Google scholar ID: https://shorturl.at/WuJQy #genetics #biotechnology #plantgenetics #plantbiotechnology #publication #bookchapter #cancerpathways #inflammationpathways

Multiple Analyses in One Test! Our #urWGS analyzes more than just #SNVs and #CNVs, to help end your patient's diagnostic odyssey and increase diagnostic yield. Replace your chromosomal #microarray, #panels, #wholeexomesequencing and more with one test! Learn more: Whole Genome Sequencing| Revvity #genetictesting #RevvityOmics #NGS #genomics #research #healthcare #rWGS #NICU #genetics http://ms.spr.ly/6042WwHnK

🔍Imagine a CRISPR library as a powerful Super Search Engine 🧬 for genetic discovery! It’s packed with thousands of sgRNA sequences, each tailored to target a specific gene🧩. Running a CRISPR library screen is like scanning through an entire genetic encyclopedia 📚 to uncover which genes are crucial for certain functions or disease processes. ⚡This high-throughput method lets you perform a sweeping search for gene function gains or losses in a single experiment, like a “genetic treasure hunt” 🏆, where essential genes emerge from the crowd. 🔬At EDITGENE, we bring cutting-edge tech and specialized expertise to the table to make sure your CRISPR library screening is efficient, precise, and ready to unlock 🔓 exciting new genetic discoveries! https://lnkd.in/gt8M8JW4 #GeneEditing #CRISPR #Knock_In #KnockOut #PointMutation #Overexpression #CellLineDevelopment #GeneticResearch #PrecisionMedicine #BiotechInnovation #CustomCellLines #MolecularBiology #ResearchTools #Genomics #EDITGENE #CrisprLibraryScreening

Accelerate your functional genomic screens with Cellecta’s high-quality CRISPR knockout libraries, designed for unbiased phenotypic screening in mammalian cells. Our pooled sgRNA libraries enable the efficient knockout of thousands of genes in a single experiment, providing the ideal solution for studying gene function, drug responses, and disease phenotypes. Key Features: - Uses the latest Brunello library gene annotations (2023) - Incorporates Cellecta’s advanced tracrRNA “HEAT” design for enhanced knockout efficiency. - Available in both plasmid and pre-packaged lentiviral forms for immediate use. Explore how Cellecta’s CRISPR knockout human, mouse, chicken or pig libraries can power your next discovery in the link below: https://lnkd.in/g5WGjY_g #Cellecta #CRISPR #GeneKnockout #PooledLibraries #Genomics #Biotechnology #ScientificResearch

Working on rare diseases? Our DECIPHER database gives you access to publicly available patient data allowing simple phenotypic and genotypic data comparisons. The latest DECIPHER database release covers a host of new features including: ➡️Integration of AlphaMissense scores from Google DeepMind to help you estimate how likely a nucleotide missense variant is to be pathogenic ➡️ Gene pages now link to the Open Targets Platform, facilitating the identification and prioritisation of potential therapeutic targets ➡️New Genome Aggregation Database (gnomAD v4.1) copy nucleotide variation (CNV) genome browser track displaying rare coding autosomal CNVs from exome sequencing of over 450,00 individuals Find out more here 👇 https://lnkd.in/eDFnQbCT #genetics #genomics #bioinformatics #raredisease

🔬#OneDayOneGenome BRIC-National Institute of Biomedical Genomics has sequenced the genome of Enterococcus faecalis OG-20, a bacterium often implicated in infections among immunocompromised patients. The E. faecalis genome, at 2.6 million base pairs and containing 2,579 genes, offers crucial insights into antibiotic resistance and virulence factors. This breakthrough could be key to developing new strategies to manage and treat infections in vulnerable populations. #AntibioticResistance #WAAW #Genomics #ResearchAndDevelopment #PublicHealth #OneHealth Rajesh Gokhale