Spectrumceuticals’ Post

Publication alert 🚨 : Our new review article on antibiotic resistance via distinct gene amplifications is out now in npj antimicrobials and resistance!!! This article nicely categorizes the different examples of how antibiotic resistance can emerge from tandem copies of genes that are coding for efflux pumps, inactivating enzymes, target modifying enzymes and target modifying enzymes. It gives a new perspective to distinct mechanisms that are involved in the evolution of antibiotic resistance and is definitely a worthwhile read. Here is the link: https://lnkd.in/eJXrwrgn #AMR #antibioticresistance #geneamplification #geneduplication #bacteria

🧬 New insights into the VILLIN (VLN) gene family in cotton! Akash Deep and Dhananjay K. Pandey explore how VLN genes are involved in stress responses in Gossypium spp. in their genome-wide analysis. 🔊 This study reveals that VLN genes are crucial for regulating the actin cytoskeleton, contributing to plant responses to environmental stresses and development. 🗝 Key findings include the identification of 14 GhVLNs in Gossypium hirsutum and their significant role in stress, hormone, and light-response mechanisms. Read more here: https://lnkd.in/exQa8CRg #PlantBiology #CottonResearch #Genomics #StressResponse #MolecularBiology #AgriculturalResearch

I assisted a client with Raynaud's syndrome by tailoring natural supplements to their genetic profile, addressing issues with blood vessel dilation and clotting. Genetic analysis revealed mutations in the NOS3 and factor V genes, impacting nitric oxide production and clotting propensity. By avoiding triggers like salicylates and introducing nitric oxide boosters and systemic enzymes, their symptoms significantly improved within a week, highlighting the importance of personalized protocols in treating unique conditions like Raynaud's. #raynauds #geneticanalysis #supplements

Summary of the CRISPR/Cas9 based steps of plant genome editing. In the first step identification of best target sites is done using various online tools and primers are designed. The target-specific sgRNA with Cas9 cassettes are cloned in binary vector. In next step cassettes are then co-transformed in vivo into the plants. The transgenic plants are confirmed for the target-specific mutations using various approaches like high-throughput sequencing approaches. The positive transformed plant is then selected for further applications and analysis.

Decreased calcium permeability caused by biallelic TRPV5 mutation leads to autosomal recessive renal calcium-wasting hypercalciuria. #RareDisease #Genetics In this study, they explored the genetic cause of hypercalciuria driven by renal Ca2+ wasting in a family of consanguineous parents and three affected individuals. Whole-exome sequencing of the proband revealed a homozygous ultra-rare variant in TRPV5(NM_019841.7:c.1792G>A; p.(Val598Met)), which encodes for a renal Ca2+-selective ion channel. The variant segregates with the three individuals with hypercalciuria. Biochemical and functional analyses on the mutated TRPV5 channel demonstrated failure to increase plasma membrane Ca2+ permeability in HEK293 cells and increased proteasomal degradation. https://lnkd.in/dWmspEHS

Sharing a case of resistant rickets that we recently published. Vitamin D-dependent rickets type 1b (VDDR1b) is a rare entity caused by recessive mutations in the CYP2R1 gene encoding the hepatic 25-hydroxylase enzyme which is responsible for conversion of inactive vitamin-D to it's 25-hydroxylated form. The condition is probably under-diagnosed as it resembles nutritional rickets clinically and biochemically, except for a tendency to relapse rapidly once treatment is stopped. The diagnosis is gratifying as treatment is easy and may prevent lifelong disability. This is the first report of the condition from Asia. Link for the full article: https://lnkd.in/eVURn4Vf

Have you ever wanted to compare antibiotic resistance data and resistance gene calls in bacteria? Now you can with NCBI Pathogen Detection's new Antibiotic Susceptibility Test (AST) Browser! Learn more: https://ow.ly/mGWQ50RtH86

❓Can genes from mango plants affect Arabidopsis? 🧬The CONSTANS-like (COL) gene family in higher plants plays important roles in flowering, abiotic stress tolerance, fruit development and ripening. 🔎 Here the authors isolated three COL genes from mango and investigated their functions. 📈 All three genes were highly expressed during flowering induction, and their expression was affected by light, with one of them showing a circadian rhythm. 📊 Overexpressing these genes in Arabidopsis showed different effects on flowering, either delayed or promoted earlier flowering depending on the gene. 📈 The overexpression increased SOD activity and proline levels, decreased malondialdehyde and improved drought and salt stress survival. 🌡️In addition to that, they interacted with several stress- and flower-related proteins. https://lnkd.in/dEzqZ2r3

The science of #nutrigenomics has unearthed an abundance of insights into varying micronutrient requirements for different gene variants. The MTHFR and APOE genes are two valuable examples of how variations impact nutrient efficacy and ultimately guide correct supplementation.   The 3X4 Blueprint Genetic test can confirm which variation of these genes your patient carries, taking out the hypothesising or assumptions when it comes to nutritional prescriptions. If you aren’t already using gene testing in your practice, please reach out to us. https://lnkd.in/d2qdtSfY 3X4 Genetics Next Biosciences #PersonalisedHealth #GeneTest #GeneticTests

'-- Next, a comparison of gene expression patterns between CR responding and non-responding strains revealed unique sets of genes and pathways and regulators of CR response. We found that responding wild strains uniquely adapted to increase mitochondrial translation coupled with oxidative phosphorylation and ATP biosynthesis under high glucose conditions. -- This data suggests that under high glucose conditions (2%), negatively responding strains might have decreased mitochondrial capacity for energy derivation by oxidative phosphorylation ... ...' Excellent - combine with ... ... https://lnkd.in/eberRK4V Excellent - combine with - '3-Hydroxybutyric acid exists in all living species, ranging from bacteria through yeast into humans' https://lnkd.in/eberRK4V