Sterilex’s Post

📢🌿 𝐀𝐠𝐫𝐨𝐓𝐚𝐥𝐤𝐬 𝟮𝟬𝟮𝟰 are coming back!   Join us and our experts on 𝐉𝐮𝐧𝐞 𝟭𝟵𝐭𝐡, from 11:00 am to 12:30 pm, on Zoom!   In this webinar, we will explore the highly relevant and timely topic of 𝐞𝐧𝐝𝐨𝐜𝐫𝐢𝐧𝐞 𝐝𝐢𝐬𝐫𝐮𝐩𝐭𝐨𝐫𝐬, covering everything from updates on data requirements to the introduction of new evaluation criteria.   We will also discuss the regulatory aspects of assessing endocrine disruptors and describe various 𝐞𝐜𝐨𝐭𝐨𝐱𝐢𝐜𝐨𝐥𝐨𝐠𝐢𝐜𝐚𝐥 𝐭𝐞𝐬𝐭𝐬 used for their evaluation in animals.   ⬇️ Register now to secure your spot, link in the first comment! #highqualitycontrols #commitment2innovation #glp #webinar #AgroTalks

#Article Detection of Unamplified E. coli O157 DNA Extracted from Large Food Samples Using a Gold Nanoparticle Colorimetric Biosensor by Emma Dester, Kaily Kao and Evangelyn C. Alocilja https://lnkd.in/ertUxv3v MDPI Michigan State University #foodbornepathogens #foodborneillness #biosensing #foodsafety #oligonucleotideprobe #openaccess #Abstract Rapid detection of foodborne pathogens such as E. coli O157 is essential in reducing the prevalence of foodborne illness and subsequent complications. Due to their unique colorimetric properties, gold nanoparticles (GNPs) can be applied in biosensor development for affordability and accessibility. In this work, a GNP biosensor was designed for visual differentiation between target (E. coli O157:H7) and non-target DNA samples. Results of DNA extracted from pure cultures indicate high specificity and sensitivity to as little as 2.5 ng/µL E. coli O157 DNA. Further, the biosensor successfully identified DNA extracted from flour contaminated with E. coli O157, with no false positives for flour contaminated with non-target bacteria. After genomic extraction, this assay can be performed in as little as 30 min. In addition, food sample testing was successful at detecting approximately 103 CFU/mL of E. coli O157 magnetically extracted from flour after only a 4 h incubation step. As a proof of concept, these results demonstrate the capabilities of this GNP biosensor for low-cost and rapid foodborne pathogen detection.

Important step to get rid of FCS in cell culture!

March is here and with it the first heralds of spring. 🌱 🌷 The first quarter is drawing to a close and with it our ZymoPURE promo. It's about time, folks! Check your Plasmid-Prep stocks and place your order by March 31st to secure your 𝟮𝟬% discount on ZymoPURE(II) kits. Use Promo-Code ＰＵＲＥ２４－３０ during checkout. 📧 As this is our anniversary year, stay informed about upcoming events and promotions by subscribing to our newsletter (link to our subscription form follows in the comments). #DNAisolation #scienceandtechnology #bioscience #zymoresearcheurope #genomics

Complete genes may pass from food to human blood. PLoS One. 2013 Jul 30;8(7):e69805. => We report evidence that meal-derived DNA fragments which are large enough to carry complete genes can avoid degradation and through an unknown mechanism enter the human circulation system (!) ''Our bloodstream is considered to be an environment well separated from the outside world and the digestive tract. According to the standard paradigm large macromolecules consumed with food cannot pass directly to the circulatory system. During digestion proteins and DNA are thought to be degraded into small constituents, amino acids and nucleic acids, respectively, and then absorbed by a complex active process and distributed to various parts of the body through the circulation system. Here, based on the analysis of over 1000 human samples from four independent studies, we report evidence that meal-derived DNA fragments which are large enough to carry complete genes can avoid degradation and through an unknown mechanism enter the human circulation system. In one of the blood samples the relative concentration of plant DNA is higher than the human DNA. The plant DNA concentration shows a surprisingly precise log-normal distribution in the plasma samples while non-plasma (cord blood) control sample was found to be free of plant DNA''.

To keep your human-based models free of other non-human components.

EPISKIN SA, a world leader in human #3Dtissue engineering for #invitro epithelial models, together with #EPISKINBRASIL, launched a new 12-well plate 🧫 of SkinEthic RHE reconstructed human epidermis. https://lnkd.in/d6BKNfQF The model ca be applied in different areas, for example #skininfection, #allergy and #omics analysis, without using animals, since the use of #alternativemethods to animal testing is one of Epikin’s mission.

ARTIGOS EM REVISTAS COM ARBITRAGEM CIENTÍFICA - INIAV The Role of Flagellum and Flagellum-Based Motility on Salmonella Enteritidis and Escherichia coli Biofilm Formation Flagellum-mediated motility has been suggested to contribute to virulence by allowing bacteria to colonize and spread to new surfaces. In Salmonella enterica and Escherichia coli species, mutants affected by their flagellar motility have shown a reduced ability to form biofilms. While it is known that some species might act as co-aggregation factors for bacterial adhesion, studies of food-related biofilms have been limited to single-species biofilms and short biofilm formation periods. To assess the contribution of flagella and flagellum-based motility to adhesion and biofilm formation, two Salmonella and E. coli mutants with different flagellar phenotypes were produced: the fliC mutants, which do not produce flagella, and the motAB mutants, which are non-motile. The ability of wild-type and mutant strains to form biofilms was compared, and their relative fitness was determined in two-species biofilms with other foodborne pathogens. Our results showed a defective and significant behavior of E. coli in initial surface colonization (p < 0.05), which delayed single-species biofilm formation. Salmonella mutants were not affected by the ability to form biofilm (p > 0.05). Regarding the effect of motility/flagellum absence on bacterial fitness, none of the mutant strains seems to have their relative fitness affected in the presence of a competing species. Although the absence of motility may eventually delay initial colonization, this study suggests that motility is not essential for biofilm formation and does not have a strong impact on bacteria’s fitness when a competing species is present. Diana Vilas Boas, Joana Castro (#INIAV), Daniela Araújo (#INIAV), Franklin L. Nóbrega, Charles W. Keevil, Nuno F. Azevedo, Maria João Vieira and Carina Almeida (#INIAV) Aceda aqui ao artigo: https://lnkd.in/dTKTZFfB #INIAV #artigo #investigação

Discover why ABI focuses on post-mortem porcine biologicals and our vital contributions to health research. Join Dr. Gary Brubaker, Founder of Animal Biotech Industries, as he unravels the world of porcine research in this insightful conversation. Visit our website to discover how ABI can elevate your research efforts and drive scientific progress. https://loom.ly/NSt-AF0 #scientificprogress #healthresearch #biotech #porcineresearch

One of the most common questions we're asked about ProvaCharge is if it kills better than PAA alone. We're fortunate to have a skilled Microbiology team and lab that allows us to scientifically answer questions we receive. The slides below showcase the power of ProvaCharge using a fluorescent Invitrogen™ LIVE/DEAD™ stain where live bacteria stain green and dead bacteria stain red-orange. Image 1, Control Slide: Lots of green (live bacteria). Image 2, After PAA (no ProvaCharge): A fair amount of green still remains. Image 3, After PAA + ProvaCharge: No green at all! Magnify the images to see individual cells and the high density of cells. ProvaCharge says lights out to biofilm bacteria! https://lnkd.in/eRvf8zc5 #biofilmcontrol #biofilmlisteria #ProvaCharge #foodprocessing