The PHERA Project’s Post

#Researchers, guanosine triphosphate cyclohydrolase 1 (GCH1) activity is allosterically regulated by phenylalanine and BH4, but with opposite effects. Elevated phenylalanine levels lead to stimulation of GCH1 activity, whereas BH4, the end product of the biosynthesis pathway, inhibits GCH1 via a negative feedback loop. Comprehensive structural studies led to the discovery of AXSP0056, an allosteric molecule that selectively occupies the BH4 binding site on GCH1, thereby inhibiting its activity. Learn more on #opnMe https://lnkd.in/eM9y8FbU #openscience #boehringer

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#drugnaming Series MAOIs #Isocarboxazid #Iso: refers to isomerism, where molecules have the same molecular formula but different structures. #Carbox: Refers to the carboxyl group (–COOH) present in the molecule. #Azid: Indicates the presence of an azide group. Join me and Atheer Alrawashdeh on our enlightening series P.S: this information are based upon personal research

In this poster presentation, we discuss how we developed and validated a sensitive LC-MS/MS assay for atropine, quantifying it across a wide range (80 pg/mL to 40 ng/mL) in rabbit plasma. Our method addresses the challenges of enantiospecific and species-specific atropinesterase, ensuring accurate achiral quantitation. Following ICH M10 guidelines, we achieved a 100% success rate in incurred sample reanalysis, confirming the robustness of our sample stabilization strategy. Watch it here: https://lnkd.in/dtUDwknU #MassSpectrometry #Bioanalysis #DrugDevelopment

Good example of fast cell sorting with HP microfluidics

What factors does your laboratory or organization consider when deciding between Targeted NGS and / or CGP? Let’s start the conversation! Pillar Biosciences Incorporated Illumina

Amidites, particularly phosphoramidites, have revolutionized #DNA synthesis. To support scientists working on nucleotide synthesis, we have curated the Mcule Amidite Library, featuring ribose-based compounds with various functionalities, as well as a dedicated #phosphoramidite selection. Explore our newly launched collection and enhance your #nucleotide synthesis projects. #mculelibraries #amidites

Congratulations to Yaowen Wu (Umeå Univ., Sweden), Herbert Waldmann (Max-Planck-Institute, Dortmund) and their research teams, for the work by A.Knyazeva et. al just published in PNAS "A chemical inhibitor of IST1-CHMP1B interaction impairs endosomal recycling and induces noncanonical LC3 lipidation"! Proud that our Chemical Proteomics Unit at the Karolinska Institute has supported such a journey with our MS-based proteomics target ID and MoA elucidation methods, particularly, with our PISA method to identify IST1 as the target of Tantalosin, and with HDX (H/D exchange) MS to map the Tantalosin binding site and the allosteric site impairing IST1-CHMP1B interaction upon binding. It has been super nice and productive to work with you, very elegant validation of the target identified by PISA as well all the multidisciplinary experiments showing not only the mode of action (MoA) of Tantalosin but how small molecules can be used as valuable tools to unravel complex biological mechanisms https://lnkd.in/gK3dgxWD #ChemicalProteomics #PISAassay #Proteomics #MassSpectrometry #Target_ID #MoA #HDXMS #bindingsite #allostericmodulation

Lucigen Corp. Endura™ Competent Cells are optimal for conserving clones that contain unstable sequences and maintaining unstable DNA. Endura™ Competent Cells are a commonly used strain for cloning sequences that suffer unwanted recombination events in other strains. Clones with inverted repeats or other sequences prone to recombination are commonly found in retroviral genes, and require cells such as Endura to be propagated stably. Available as chemically competent or electrocompetent cells, Endura Competent Cells are an excellent value Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise. Reach out to your Avantor Representative for more information and quote information. #avantorsetsscienceinmotion #researchanddevelopment #cellculture #cellbiology #academia #umanitoba #competentcells https://lnkd.in/e7xaWFdF