CE Discussion Group

The Dutch #CEDiscussionGroup is next week! Today is the last day of registration, don’t miss out and register through the link below 👇  🗓️ Date: 14th of October 2024   🕛 Time: 09:30 - 17:00  📌 Location: @Isogen Life Science in Utrecht  ✒ https://lnkd.in/e_ZJsSDC    We would like to put our final speaker in the spotlight: Ann-Katrin Schwenzer!  Ann-Katrin Schwenzer is a PhD student in analytical chemistry at Aalen University (Germany), in the group of Prof. Dr. Christian Neusüß. Her research focuses on the characterization of the conformation of proteoforms of biopharmaceutical proteins by capillary electrophoresis-ion mobility mass spectrometry.  The title of her talk is ✨ Native CE of mAb proteoforms coupled to MS under native and denaturing SL conditions ✨ Therapeutic antibodies play an important part in the treatment of diseases. Monoclonal antibodies (mAbs) are complex molecules that undergo various post-translational modifications, such as charge heterogeneity. Monitoring of charge variants is essential to ensure efficacy and safety of the biopharmaceutical. Capillary electrophoresis (CE) is an appropriate technique for routine and rapid charge variant analysis of biopharmaceuticals, however, the typically used non-volatile background electrolytes makes hyphenation to mass spectrometry (MS) challenging. Here, we present a CE method for separating mAb charge variants under native conditions with subsequent online MS analysis. For CE-MS coupling, different sheath liquids have been compared and their influence on MS spectra was investigated.  For more information on the talk and the event, go to https://lnkd.in/dZRHVFDJ.  We hope to see you there! 🙋♀️ 🙋♂️    Elena Dominguez Vega Rob Haselberg Eline van den Berg Kevin Jooß Debbie van der Burg 

It is almost time for the Dutch #CEDiscussionGroup and we are excited to introduce yet another speaker! 🤩        Ruben Cageling is working on his PhD project at Polpharma Biologics and Vrije Universiteit Amsterdam (VU Amsterdam). His research focuses on developing novel high-throughput strategies for the development of biosimilars, using microfluidic capillary electrophoresis and high-resolution mass spectrometry techniques. In his talk titled ✨ Rapid clone screening of biosimilar candidates using microfluidic CE-MS ✨ he will tell us more about a microfluidic capillary electrophoresis - mass spectrometry (MCE-MS) method for the rapid screening of mAb biosimilar candidates by assessing charge variants and glycosylation simultaneously. The optimized method is sensitive, fast, cell-line discriminating, and transferable. For more information about this talk, see https://lnkd.in/dZRHVFDJ. Registration will close on 07 October, don’t miss out and register now! 👇  🗓️ Date: 14th of October 2024   🕛 Time: 09:30 - 17:00  📌 Location: @Isogen Life Science in Utrecht  ✒ https://lnkd.in/e_ZJsSDC    We hope to see you there! 🙋♂️ 🙋♀️  Elena Dominguez Vega Rob Haselberg Eline van den Berg Kevin Jooß Debbie van der Burg 

Are you ready for the Dutch #CEDiscussionGroup on 14 October in Utrecht? 🤩    On this beautiful morning it is time to put the spotlight on our next speaker: Andrea Capuano. Andrea holds a PhD in microtechnologies and is currently a Marie Curie Post-doc researcher at EXIT071, an innovative spinoff company of Leiden University in the Netherlands. His expertise includes miniaturized device development, wearable medical device design, and testing of container closure systems for drugs. His talk is titled ✨Depletion-zone isotachophoresis (dzITP) of extracellular vesicles: a separation method suitable for biomarker discovery applications that can increase purity over current techniques✨     About the lecture  Exosomes are nanovesicles produced by cells, crucial for cell-to-cell communication, which have a mediating role, through different biomarkers, in the development of metabolic syndromes, malignancies, or neurodegenerative diseases. By leveraging the principle of Depletion-zone isotachophoresis (dzITP), at EXIT071, we are developing a microchip technology that utilizes electric fields to isolate exosomes from blood plasma, facilitating comprehensive analyses such as lipidomics and metabolomics. Our method further purifies exosome samples beyond conventional techniques like size exclusion chromatography and tangential flow filtration. This innovation promises to revolutionize diagnostics and enable personalized therapies, offering faster and less invasive solutions for the future. For more information about Andrea or the event, see https://lnkd.in/dZRHVFDJ. Registration will close on 07 October, don’t miss out and register now! 👇  🗓️ Date: 14th of October 2024   🕛 Time: 09:30 - 17:00  📌 Location: @Isogen Life Science in Utrecht  ✒ https://lnkd.in/e_ZJsSDC    We hope to see you there! 🙋♂️ 🙋♀️  Elena Dominguez Vega Rob Haselberg Eline van den Berg Kevin Jooß Debbie van der Burg

🔬 Join Us for an Engaging Panel Discussion on Capillary Electrophoresis! 🔎 We are thrilled to announce an upcoming panel discussion featuring experts from industry, vendors, and academia, all focused on the transformative role of Capillary Electrophoresis in today’s scientific landscape! We have invited Rob Haselberg, Cari Sänger - van de Griend and Martin Donker to take a seat in our panel and to share their insights while leaving you with the opportunity to ask all your questions freely.  🗓️ Date: 14th of October 2024   🕛 Time: 09:30 - 17:00  📌 Location: Isogen Life Science in Utrecht This is a unique opportunity to: 🎤 Hear insights from leading professionals  🤝 Network with key players in the field  💡 Explore challenges, advancements, and future trends  Whether you're an experienced researcher or just curious about the technology, this panel promises valuable perspectives and lively discussions. 👉 Secure your spot today: https://lnkd.in/e_ZJsSDC  Feel free to tag anyone who would benefit from this discussion! Let’s innovate and collaborate to push the boundaries of capillary electrophoresis! Elena Dominguez Vega Rob Haselberg Eline van den Berg Kevin Jooß Debbie van der Burg #CapillaryElectrophoresis #PanelDiscussion #Networking #Innovation #ScienceCollaboration #AnalyticalChemistry 

Good morning everyone, the Dutch #CEDiscussionGroup is approaching soon! 🌟 We are happy to introduce our second speaker: Aurore Boclinville! Aurore is a PhD student in Biomedical and Pharmaceutical Sciences at the University of Liège (Belgium). She works in the Laboratory for the Analysis of Medicines under the supervision of Dr. Anne-Catherine Servais. Her research focuses on the development of innovative analytical techniques to characterize human papillomavirus.   Her talk is titled ✨ 𝗜𝗻𝘁𝗲𝗿𝗲𝘀𝘁 𝗼𝗳 𝗜𝗺𝗮𝗴𝗲𝗱 𝗖𝗮𝗽𝗶𝗹𝗹𝗮𝗿𝘆 𝗜𝘀𝗼𝗲𝗹𝗲𝗰𝘁𝗿𝗶𝗰 𝗙𝗼𝗰𝘂𝘀𝗶𝗻𝗴 𝗳𝗼𝗿 𝘁𝗵𝗲 𝗖𝗵𝗮𝗿𝗮𝗰𝘁𝗲𝗿𝗶𝘇𝗮𝘁𝗶𝗼𝗻 𝗼𝗳 𝗛𝘂𝗺𝗮𝗻 𝗣𝗮𝗽𝗶𝗹𝗹𝗼𝗺𝗮𝘃𝗶𝗿𝘂𝘀 𝗩𝗶𝗿𝘂𝘀-𝗟𝗶𝗸𝗲 𝗣𝗮𝗿𝘁𝗶𝗰𝗹𝗲𝘀 ✨ in which she will tell us more about the characterization and quality control of virus-like particles (VLPs) during the development and production of effective vaccines using imaged capillary isoelectric focusing (icIEF).  See https://lnkd.in/dZRHVFDJ for more information on Aurore and her talk.  Registration is still open (and free!) 👇 🗓️ Date: 14th of October 2024   🕛 Time: 09:30 - 17:00  📌 Location: @Isogen Life Science in Utrecht  ✒ https://lnkd.in/e_ZJsSDC    Elena Dominguez Vega Rob Haselberg Eline van den Berg Kevin Jooß Debbie van der Burg 

Next month is the next Dutch #CEDiscussionGroup event and we are excited to introduce our first speaker! 🤩 𝗫𝗶𝗮𝗼𝘅𝗶 𝗭𝗵𝗮𝗻𝗴 is Application Manager in Pharma and Biopharma vertical marketing team, Chromatography and Mass Spectrometry Division, Thermo Fisher Scientific, China. Xiaoxi is managing collaborations with China regulatory authority, biopharma KOLs and leading the iCIEF- MS collaboration with Advanced Electrophoresis Solutions Ltd. (AES). She has 10+ publications/orals/posters in iCIEF-MS for charge variant analysis and biotherapeutic characterization since 2021. Xiaoxi will present about✨ 𝗶𝗰𝗜𝗘𝗙 - 𝗠𝗦 𝗼𝗻𝗹𝗶𝗻𝗲 𝗰𝗼𝘂𝗽𝗹𝗶𝗻𝗴 𝗳𝗼𝗿 𝗰𝗵𝗮𝗿𝗴𝗲 𝗵𝗲𝘁𝗲𝗿𝗼𝗴𝗲𝗻𝗲𝗶𝘁𝘆 𝗮𝗻𝗮𝗹𝘆𝘀𝗶𝘀 𝗼𝗳 𝗺𝗔𝗯, 𝗿𝗲𝗰𝗼𝗺𝗯𝗶𝗻𝗮𝗻𝘁 𝗽𝗿𝗼𝘁𝗲𝗶𝗻 𝘃𝗮𝗰𝗰𝗶𝗻𝗲 𝗮𝗻𝗱 𝗔𝗗𝗖 𝘂𝘀𝗶𝗻𝗴 𝗱𝗲𝗻𝗮𝘁𝘂𝗿𝗲𝗱 𝗮𝗻𝗱 𝗻𝗮𝘁𝗶𝘃𝗲 𝗠𝗦 ✨where she will tell us more about online icIEF-MS under denaturing condition for mAb and recombinant protein vaccine charge variant analysis, and near-native condition to analyze charge heterogeneity of polatuzumab vedotin, a latest-generation cysteine-linked ADC, using CEinfinite iCIEF platform and Thermo Scientific™ Orbitrap Exploris™ 240 mass spectrometry under Thermo Scientific™ Chromeleon™ software control. More information on the speaker and the event can be found on: https://lnkd.in/dZRHVFDJ Save the date and register to make sure you have a spot! 👇 🗓️ Date: 14th of October 2024  🕛 Time: 09:30 - 17:00 📌 Location: Isogen Life Science in Utrecht ✒ https://lnkd.in/e_ZJsSDC We hope to see you there! 🙋♂️ 🙋♀️ Elena Dominguez Vega Rob Haselberg Eline van den Berg Kevin Jooß Debbie van der Burg

𝐒𝐀𝐕𝐄 𝐓𝐇𝐄 𝐃𝐀𝐓𝐄: 14th of October 2024 📌 CEnthousiasts, we hope you are all well and had a sunny and relaxed summer vacation! 😎 Hopefully summer gave the energy to tackle the rest of the year and eagerness to gain some #CE knowledge! This fall the CE Discussion Group will have another meeting. This edition will be a full day program held on October 14th, at and promoted by Isogen Life Science in Utrecht. Register for free(!) to make sure you have a spot!  ✏  https://lnkd.in/e_ZJsSDC 𝐖𝐡𝐚𝐭 𝐭𝐨 𝐞𝐱𝐩𝐞𝐜𝐭: 🧑🔬 Several #scientists excited to share their work and knowledge. 🕵 Plenty of opportunity to discuss during round table and #troubleshooting sessions. 👥 Network with fellow scientists in your CE community with some drinks. 🆕 Also learn about the latest trends and the newest innovations of other disciplines! We have a first program to share, with exciting details to come! So make sure you follow us on LinkedIn, or register for our CEDG day to stay up-to-date! We hope to see you there! 🖐 Debbie van der Burg Elena Dominguez Vega Rob Haselberg Kevin Jooß Eline van den Berg

Dear CE enthusiasts, Thank you to all speakers and attendees for another interesting and open discussion meeting! We are looking back at a nice and successful afternoon of sharing our thoughts on #CE. We don't want it to end here: Mark your calendars already for the 14th of October 📆 A new full CEDG day is coming in Utrecht at and promoted by Isogen Life Science! ⭐️

The Dutch #CEDiscussionGroup meeting is next week! There is still time to register: so, save the date and join us at Lab Technology 2024! With the sun finally coming through these days, we would like to put our third and final speaker in its spotlight: Dr. Guinevere S.M. Lageveen-Kammeijer! ☀ She is an Assistant Professor University of Groningen, Analytical Biochemistry group of the Groningen Research Institute of Pharmacy Her talk is entitled ⭐ Exploring the Sweet Side of Cells by CE-MS ⭐ 𝐀𝐛𝐨𝐮𝐭 𝐭𝐡𝐞 𝐥𝐞𝐜𝐭𝐮𝐫𝐞  An altered glycosylation profile is a hallmark of many diseases, including cancer. However, to establish better diagnostic, prognostic as well as therapeutic treatments for cancer patients, a more thorough investigation is needed on the glycomic intratumoral heterogeneity (e.g. primary versus metastatic tumors), as tumors consists of a network of cell populations that are interconnected in tissue matrix. In this lecture, an innovative and miniaturized high-sensitivity glycomics CE-MS workflow will be presented that only requires minimal sample amounts (e.g., a few cells or 100 pg of protein weight) compared to conventional glycomic platforms that require 20,000 up to 100,000 cells. ✒ https://lnkd.in/deBuWD85 ✔ Register as: SAC CE 🗓️ Date: 28th of May 2024 🕛 Time: 12:15 - 17:15 📌 Location: Congrescentrum De Reehorst, Ede You will have 𝐟𝐫𝐞𝐞 access to the whole event! As #CEDG we will kick-off our session with lunch and a coffee chat and finalize the day with drinks 🥂 For more information on the speaker and the event: https://lnkd.in/e8d4aNxR We hope to see you there! 🙋♀️ 🙋♂️

The Dutch #CEDiscussionGroup and Lab Technology are approaching soon! Today we would like to introduce another speaker while registration is still open: 🗓️ Date: 28th of May 2024 🕛 Time: 12:15 - 17:15 📌 Location: Congrescentrum De Reehorst, Ede ✒ https://lnkd.in/deBuWD85 ✔ Register as: SAC CE You will have access to the whole event, and free of charge! We are happy to share our second speaker: Annika van der Zon! She is a PhD candidate at the group of Andrea Gargano (HIMS, Analytical Chemistry Group) and within Center of Analytical Sciences Amsterdam (CASA) at University of Amsterdam Her talk is entitled ⭐ Charge Variants Separation of Intact Monoclonal Antibodies at pH 5 using Nanoflow Sheath Liquid Capillary Electrophoresis-Mass Spectrometry ⭐ 𝐀𝐛𝐨𝐮𝐭 𝐭𝐡𝐞 𝐥𝐞𝐜𝐭𝐮𝐫𝐞  Capillary electrophoresis (CE) is a powerful technique to separate charge variants of monoclonal antibodies (mAbs), to date however, methods are mostly CZE-UV based. In this study, we developed a nanoflow sheath liquid CZE-MS method for mAbs. The method was developed at pH 5 using neutral-coated capillaries, offering improved performance with respect to typical pH 3 conditions used in CZE-MS. The method has a comparative performance as the EACA method of He et al. (2011) but has the advantage of being hyphenated with MS allowing for in-depth mAb charge variants characterization. More information on the speaker and the event can be found on: https://lnkd.in/e8d4aNxR We hope to see you there! 🙋♂️ 🙋♀️