Helix Biostructures

At Helix, we thrive on uncovering the structural secrets of key enzymes, and the Cryo-EM structures of CerS6 captured in this study offer a fascinating glimpse into ceramide biosynthesis. CerS6 synthesizes C16 ceramides, which are linked to metabolic disorders like obesity, diabetes, and NASH. This study elucidates its ping-pong reaction mechanism, utilizing a histidine nucleophile for substrate conversion (https://loom.ly/MlmDFSk ). Importantly, the findings highlight how fumonisin B1 inhibits CerS6, laying a foundation for designing specific CerS6 inhibitors. These insights mark a critical step toward therapeutics targeting ceramide-related metabolic dysfunctions. Fascinating! Did you know... our Cryo-EM expertise is at your disposal to catapult your projects ahead? Reach out to us today to see how we can help! (www.HelixBioStructures.com) #cryoem #crystallization #crystallography #drugdiscovery #drugdesign #protein #proteins #dna #rna #antibody #antibodies #science #nature #research #bio #bionetwork #biotechnology #biotech #bioscience #biotechnologyindustry #biopharma #drugdesign #drugdevelopment #pharmaceuticals #pharma #drug #lifesciences #lifescience

Our biophysical characterization of these tacos: stable, tasty, and well-folded 🌮⚡🔬 #TacoTuesday #HelixBiostructures

How do you determine optimal constructs for X-ray crystallography and cryo-EM? An increasingly popular technique is SAXS, which is particularly useful in detecting flexible or disordered regions that may hinder crystallization or cryo-EM analysis. Helix has written a white paper entitled, "Small-Angle X-ray Scattering (SAXS): Applications in Protein, RNA/DNA, and Nanostructure Analysis." Read our white paper here: https://loom.ly/TrQoVYw

Check out Helix's new website! Today, we highlight our cryo-EM structure determination services. Helix offers full-service cryo-EM on protein complexes, viruses, antibodies, membrane proteins, ion channels and more, with grid screening, data collection, and high-resolution results—on state-of-the-art Glacios™ 2 and Krios™ microscopes—all at competitive rates and fast turnaround times. Watch a video describing our capabilities here: https://loom.ly/wR-SlBU

Check out Helix's new website! Today, we focus on the Protein Crystallography portion of our gene-to-structure services. At Helix, we are world experts in protein crystallization and structure determination services. We use high-purity proteins (often from our own production team) and an automated platform to quickly generate and optimize diffraction-quality crystals, even for challenging conditions. Watch a video describing our capabilities here: https://loom.ly/uVczFX4

Check out Helix’s newly launched website! Today, we’re spotlighting our Biophysical Characterization services, tailored to support your protein and drug discovery needs. Need to assess protein quality, stability, folding, or drug-target interactions? Helix provides flexible, cost-effective access to a range of industry-leading analytical methods and techniques. Protein Characterization: • TSA/DSF (Thermal Shift Assay) • MP (Mass Photometry) • DLS (Dynamic Light Scattering) • SEC-MALS (SEC with Multi-Angle Light Scattering) • CD (Circular Dichroism) Binding Characterization: • TSA/DSF (Thermal Shift Assay) • MST (Microscale Thermophoresis) • ITC (Isothermal Titration Calorimetry) • SPR (Surface Plasmon Resonance) • BLI (Biolayer Interferometry) Visit our website to learn how we can support your next project! Learn more here: https://loom.ly/-KUcTNg

Check out Helix’s newly launched website! Today, we’re spotlighting our Biophysical Characterization services, tailored to support your protein and drug discovery needs. Need to assess protein quality, stability, folding, or drug-target interactions? Helix provides flexible, cost-effective access to a range of industry-leading analytical methods and techniques. Protein Characterization: • TSA/DSF (Thermal Shift Assay) • MP (Mass Photometry) • DLS (Dynamic Light Scattering) • SEC-MALS (SEC with Multi-Angle Light Scattering) • CD (Circular Dichroism) Binding Characterization: • TSA/DSF (Thermal Shift Assay) • MST (Microscale Thermophoresis) • ITC (Isothermal Titration Calorimetry) • SPR (Surface Plasmon Resonance) • BLI (Biolayer Interferometry) Visit our website to learn how we can support your next project! Learn more here: https://loom.ly/-KUcTNg

Check out Helix's new website! Today, the spotlight is on Protein Production, the first service we provide in our gene-to-structure services. Helix's expertise is in producing structure-grade proteins (soluble and membrane) at 95% purity or higher using licensed cell lines (Bacteria, Insect, Mammalian, Yeast). Watch a video detailing our capabilities here: https://loom.ly/J3EPjAY

At Helix, we are deeply fascinated by GPCR biology, and the structural insights into GPR55 unveiled in this study offer exciting opportunities for therapeutic innovation. Once thought to be a cannabinoid receptor, GPR55 has emerged as a distinct target with unique ligand recognition and signaling mechanisms. This study identifies lysophosphatidylinositol (LPI) as the authentic ligand for GPR55 and demonstrates its signaling through the G13 pathway ( https://loom.ly/PbCWLUE ). Using cryo-EM, researchers resolved high-resolution structures of LPI- and AM251-bound GPR55, revealing a binding mode distinct from cannabinoid receptors. These findings pave the way for developing GPR55 modulators to address metabolic and neurological disorders, including diabetes and Parkinson’s disease. And you guessed it… this article is access free! Did you know... Our cryo-EM and X-ray crystallization services equip clients with the necessary tools to elucidate the structural shape and attributes of their proteins, antibodies, and other biologicals? Success is achieved through the combination of capability, capacity, and expertise. Contact us now to discuss how we can assist in bringing your project to fruition! (www.HelixBioStructures.com) #cryoem #crystallization #crystallography #drugdiscovery #drugdesign #protein #proteins #dna #rna #antibody #antibodies #science #nature #research #bio #bionetwork #biotechnology #biotech #bioscience #biotechnologyindustry #biopharma #drugdesign #drugdevelopment #pharmaceuticals #pharma #drug #lifesciences #lifescience

Check out Helix's new website! Today, we're highlighting the state-of-the-art equipment that Helix has invested in to provide its clients with protein production, biophysical characterization, X-ray crystallography, and cryo-EM services. Have you ever wondered what instrumentation Helix utilizes for this gene-to-structure work? View our specific instruments and technologies here: https://loom.ly/Op-QwbA